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gene exp hmox1 mm00516005 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp hmox1 mm00516005 m1
    Human rA1M protects kidney function but no effect on cardiac hypertrophy. ( a ) Albumin/Creatinine ratio (ACR) analysis of urine from pregnant females showed an increase of ACR at late-gestation in the preeclamptic females, which was absent in the rA1M-treated group. Shown is the mean ACR normalised to Ctrl-buff values for each gestation period, and n = number of females analysed. ( b ) Gene expression levels <t>for</t> <t>HO-1</t> , CAT and SOD2 , normalised to the HPRT -gene levels in kidney from PE-buff and PE-A1M females, demonstrating significant reduction of HO-1 and CAT expression (*p = 0.04; **p = 0.04) after rA1M-treatment. The line represents the median and n = number of females analysed. ( c ) Preeclamptic females showed increased heart weight compared to Ctrl-buff at 17.5 dpc (*p = 0.002), which could not be alleviated by rA1M treatment (PE-A1M vs Ctrl-buff; **p = 0.008). Control groups showed similar heart weight as non-pregnant females. The line represents the median and n = number of females analysed.
    Gene Exp Hmox1 Mm00516005 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+specific+analysis+gsa+algorithm/Gene+Exp%2E+Hmox1%2C+Mm00516005_m1/pmc06561956-206-30-40
    Average 99 stars, based on 1 article reviews
    gene exp hmox1 mm00516005 m1 - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Alpha-1 microglobulin as a potential therapeutic candidate for treatment of hypertension and oxidative stress in the STOX1 preeclampsia mouse model"

    Article Title: Alpha-1 microglobulin as a potential therapeutic candidate for treatment of hypertension and oxidative stress in the STOX1 preeclampsia mouse model

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-44639-9

    Human rA1M protects kidney function but no effect on cardiac hypertrophy. ( a ) Albumin/Creatinine ratio (ACR) analysis of urine from pregnant females showed an increase of ACR at late-gestation in the preeclamptic females, which was absent in the rA1M-treated group. Shown is the mean ACR normalised to Ctrl-buff values for each gestation period, and n = number of females analysed. ( b ) Gene expression levels for HO-1 , CAT and SOD2 , normalised to the HPRT -gene levels in kidney from PE-buff and PE-A1M females, demonstrating significant reduction of HO-1 and CAT expression (*p = 0.04; **p = 0.04) after rA1M-treatment. The line represents the median and n = number of females analysed. ( c ) Preeclamptic females showed increased heart weight compared to Ctrl-buff at 17.5 dpc (*p = 0.002), which could not be alleviated by rA1M treatment (PE-A1M vs Ctrl-buff; **p = 0.008). Control groups showed similar heart weight as non-pregnant females. The line represents the median and n = number of females analysed.
    Figure Legend Snippet: Human rA1M protects kidney function but no effect on cardiac hypertrophy. ( a ) Albumin/Creatinine ratio (ACR) analysis of urine from pregnant females showed an increase of ACR at late-gestation in the preeclamptic females, which was absent in the rA1M-treated group. Shown is the mean ACR normalised to Ctrl-buff values for each gestation period, and n = number of females analysed. ( b ) Gene expression levels for HO-1 , CAT and SOD2 , normalised to the HPRT -gene levels in kidney from PE-buff and PE-A1M females, demonstrating significant reduction of HO-1 and CAT expression (*p = 0.04; **p = 0.04) after rA1M-treatment. The line represents the median and n = number of females analysed. ( c ) Preeclamptic females showed increased heart weight compared to Ctrl-buff at 17.5 dpc (*p = 0.002), which could not be alleviated by rA1M treatment (PE-A1M vs Ctrl-buff; **p = 0.008). Control groups showed similar heart weight as non-pregnant females. The line represents the median and n = number of females analysed.

    Techniques Used: Gene Expression, Expressing, Control

    Related Articles

    Expressing:

    Article Title: Multi-organ toxicity of ultrafine particles derived from air pollution: a sub-chronic exposure study in mice.
    Article Snippet: Although air pollution-derived ultrafine particles (UFP) can reach extrapulmonary organs, current understanding of their distribution and toxicodynamics remains scarce and largely focused on the lung.. In this work, we conducted, to the best of our knowledge, the first in vivo study using a multi-organ approach to assess both the toxicokinetics (i.e., biodistribution) and the toxicodynamics (i.e., oxidative stress, inflammation) of air pollution-derived UFP collected in an urban environment in mice sub-chronically exposed.. The intrinsic oxidative potential (OP) of UFP was assessed prior to sub-chronic exposure of Balb/cJRj mice to 0, 10, or 30 μg of UFP/40 μL of sterile saline for 3 months.

    Article Title: Renoprotective effects of human L-type fatty acid-binding protein (hL-FABP) in rhabdomyolysis-induced acute kidney injury.
    Article Snippet: Real-time polymerase chain reaction (PCR) AR TIC LE IN PR ES S Total RNA was extracted from kidney tissues using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and subsequently reverse-transcribed into cDNA using the PrimeScriptTM RT Reagent Kit (Perfect Real Time) (Takara Bio Inc., Shiga, Japan). .. The mRNA expression levels of heme oxygenase-1 (HO-1, Mm00516005_m1) and 18S ribosomal RNA (18S, Mm03928990) were measured using TaqMan real-time PCR with StepOnePlusTM Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). ..

    Article Title: Renoprotective effects of human L-type fatty acid-binding protein (hL-FABP) in rhabdomyolysis-induced acute kidney injury
    Article Snippet: Total RNA was extracted from kidney tissues using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and subsequently reverse-transcribed into cDNA using the PrimeScriptTM RT Reagent Kit (Perfect Real Time) (Takara Bio Inc., Shiga, Japan). .. The mRNA expression levels of heme oxygenase-1 (HO-1, Mm00516005_m1) and 18 S ribosomal RNA (18 S, Mm03928990) were measured using TaqMan real-time PCR with StepOnePlusTM Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Multi-organ toxicity of ultrafine particles derived from air pollution: a sub-chronic exposure study in mice.
    Article Snippet: Although air pollution-derived ultrafine particles (UFP) can reach extrapulmonary organs, current understanding of their distribution and toxicodynamics remains scarce and largely focused on the lung.. In this work, we conducted, to the best of our knowledge, the first in vivo study using a multi-organ approach to assess both the toxicokinetics (i.e., biodistribution) and the toxicodynamics (i.e., oxidative stress, inflammation) of air pollution-derived UFP collected in an urban environment in mice sub-chronically exposed.. The intrinsic oxidative potential (OP) of UFP was assessed prior to sub-chronic exposure of Balb/cJRj mice to 0, 10, or 30 μg of UFP/40 μL of sterile saline for 3 months.

    Article Title: Renoprotective effects of human L-type fatty acid-binding protein (hL-FABP) in rhabdomyolysis-induced acute kidney injury.
    Article Snippet: Real-time polymerase chain reaction (PCR) AR TIC LE IN PR ES S Total RNA was extracted from kidney tissues using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and subsequently reverse-transcribed into cDNA using the PrimeScriptTM RT Reagent Kit (Perfect Real Time) (Takara Bio Inc., Shiga, Japan). .. The mRNA expression levels of heme oxygenase-1 (HO-1, Mm00516005_m1) and 18S ribosomal RNA (18S, Mm03928990) were measured using TaqMan real-time PCR with StepOnePlusTM Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). ..

    Article Title: Renoprotective effects of human L-type fatty acid-binding protein (hL-FABP) in rhabdomyolysis-induced acute kidney injury
    Article Snippet: Total RNA was extracted from kidney tissues using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and subsequently reverse-transcribed into cDNA using the PrimeScriptTM RT Reagent Kit (Perfect Real Time) (Takara Bio Inc., Shiga, Japan). .. The mRNA expression levels of heme oxygenase-1 (HO-1, Mm00516005_m1) and 18 S ribosomal RNA (18 S, Mm03928990) were measured using TaqMan real-time PCR with StepOnePlusTM Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). ..

    Article Title: Bach2 -deficient mice are prone to autoimmune pancreatitis but protected from high-fat diet-induced fatty liver disease
    Article Snippet: RNA was reverse transcribed into cDNA using TaqMan reverse transcription reagents (Thermo Fisher Scientific, Waltham, MA, USA), 250 ng RNA per sample, and random priming. .. For quantification of target cDNA levels using real-time PCR, a qPCR MasterMix from Eurogentec (Seraing, Liège, Belgium) and the following mouse-specific TaqMan TM gene expression assays with fluorescently labeled MGB probes (Thermo Fisher Scientific) were used: Mm00477784_m (nuclear factor erythroid 2 related factor 2, Nrf2 ), Mm00516005_m (heme oxygenase 1, Hmox1/Ho-1 ), and Mm99999915_g1 (glyceraldehyde-3-phosphate dehydrogenase, Gapdh , house-keeping gene control). .. Polymerase chain reaction (PCR) was performed using the ViiA 7 sequence detection system (Thermo Fisher Scientific).

    Software:

    Article Title: Multi-organ toxicity of ultrafine particles derived from air pollution: a sub-chronic exposure study in mice.
    Article Snippet: Although air pollution-derived ultrafine particles (UFP) can reach extrapulmonary organs, current understanding of their distribution and toxicodynamics remains scarce and largely focused on the lung.. In this work, we conducted, to the best of our knowledge, the first in vivo study using a multi-organ approach to assess both the toxicokinetics (i.e., biodistribution) and the toxicodynamics (i.e., oxidative stress, inflammation) of air pollution-derived UFP collected in an urban environment in mice sub-chronically exposed.. The intrinsic oxidative potential (OP) of UFP was assessed prior to sub-chronic exposure of Balb/cJRj mice to 0, 10, or 30 μg of UFP/40 μL of sterile saline for 3 months.

    Gene Expression:

    Article Title: Oral Deuterated Docosahexaenoic Acid Protects Against Onset and Progression of RPE Degeneration in a Mouse Model of Chronic Oxidative Stress
    Article Snippet: Taqman Reverse Transcription Reagents were used to synthesize cDNA on a Veritri Thermal Cycler (Applied Biosystems, Foster City, CA, USA). .. Gene expression was measured using Taqman probes (Applied Biosystems, Foster City, CA, USA): Gapdh (endogenous control, Mm99999915_g1), Rho (rhodopsin, Mm00520345_m1), Opn1sw (short-wave cone opsin, Mm00432058_m1), Opn1mw (medium-wave cone opsin, Mm00433560_m1), Hmox1 (heme oxygenase 1, Mm00516005_m1), and Rpe65 (retinoid isomerohydrolase RPE65, Mm00504133_m1). .. The qPCRs were run in technical triplicate on a 7500 Fast Real-time PCR system (Applied Biosystems, Foster City, CA, USA).

    Article Title: Bach2 -deficient mice are prone to autoimmune pancreatitis but protected from high-fat diet-induced fatty liver disease
    Article Snippet: RNA was reverse transcribed into cDNA using TaqMan reverse transcription reagents (Thermo Fisher Scientific, Waltham, MA, USA), 250 ng RNA per sample, and random priming. .. For quantification of target cDNA levels using real-time PCR, a qPCR MasterMix from Eurogentec (Seraing, Liège, Belgium) and the following mouse-specific TaqMan TM gene expression assays with fluorescently labeled MGB probes (Thermo Fisher Scientific) were used: Mm00477784_m (nuclear factor erythroid 2 related factor 2, Nrf2 ), Mm00516005_m (heme oxygenase 1, Hmox1/Ho-1 ), and Mm99999915_g1 (glyceraldehyde-3-phosphate dehydrogenase, Gapdh , house-keeping gene control). .. Polymerase chain reaction (PCR) was performed using the ViiA 7 sequence detection system (Thermo Fisher Scientific).

    Control:

    Article Title: Oral Deuterated Docosahexaenoic Acid Protects Against Onset and Progression of RPE Degeneration in a Mouse Model of Chronic Oxidative Stress
    Article Snippet: Taqman Reverse Transcription Reagents were used to synthesize cDNA on a Veritri Thermal Cycler (Applied Biosystems, Foster City, CA, USA). .. Gene expression was measured using Taqman probes (Applied Biosystems, Foster City, CA, USA): Gapdh (endogenous control, Mm99999915_g1), Rho (rhodopsin, Mm00520345_m1), Opn1sw (short-wave cone opsin, Mm00432058_m1), Opn1mw (medium-wave cone opsin, Mm00433560_m1), Hmox1 (heme oxygenase 1, Mm00516005_m1), and Rpe65 (retinoid isomerohydrolase RPE65, Mm00504133_m1). .. The qPCRs were run in technical triplicate on a 7500 Fast Real-time PCR system (Applied Biosystems, Foster City, CA, USA).

    Article Title: Bach2 -deficient mice are prone to autoimmune pancreatitis but protected from high-fat diet-induced fatty liver disease
    Article Snippet: RNA was reverse transcribed into cDNA using TaqMan reverse transcription reagents (Thermo Fisher Scientific, Waltham, MA, USA), 250 ng RNA per sample, and random priming. .. For quantification of target cDNA levels using real-time PCR, a qPCR MasterMix from Eurogentec (Seraing, Liège, Belgium) and the following mouse-specific TaqMan TM gene expression assays with fluorescently labeled MGB probes (Thermo Fisher Scientific) were used: Mm00477784_m (nuclear factor erythroid 2 related factor 2, Nrf2 ), Mm00516005_m (heme oxygenase 1, Hmox1/Ho-1 ), and Mm99999915_g1 (glyceraldehyde-3-phosphate dehydrogenase, Gapdh , house-keeping gene control). .. Polymerase chain reaction (PCR) was performed using the ViiA 7 sequence detection system (Thermo Fisher Scientific).

    other:

    Article Title: NRF2 deficit prevents pathologic Tau seeding and spreading in an induced tauopathy mouse model
    Article Snippet: Heme oxygenase 1 , Hmox1 , Mm00516005_m1.

    Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis
    Article Snippet: Hmox-1 , Mm00516005_m1.

    Labeling:

    Article Title: Bach2 -deficient mice are prone to autoimmune pancreatitis but protected from high-fat diet-induced fatty liver disease
    Article Snippet: RNA was reverse transcribed into cDNA using TaqMan reverse transcription reagents (Thermo Fisher Scientific, Waltham, MA, USA), 250 ng RNA per sample, and random priming. .. For quantification of target cDNA levels using real-time PCR, a qPCR MasterMix from Eurogentec (Seraing, Liège, Belgium) and the following mouse-specific TaqMan TM gene expression assays with fluorescently labeled MGB probes (Thermo Fisher Scientific) were used: Mm00477784_m (nuclear factor erythroid 2 related factor 2, Nrf2 ), Mm00516005_m (heme oxygenase 1, Hmox1/Ho-1 ), and Mm99999915_g1 (glyceraldehyde-3-phosphate dehydrogenase, Gapdh , house-keeping gene control). .. Polymerase chain reaction (PCR) was performed using the ViiA 7 sequence detection system (Thermo Fisher Scientific).



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