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gene exp tbp hs00427620 m1  (Thermo Fisher)


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    Thermo Fisher gene exp tbp hs00427620 m1
    Gene Exp Tbp Hs00427620 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+specific+analysis+gsa+algorithm/Gene+Exp%2E+TBP%2C+Hs00427620_m1/pm38757249-142-29-11
    Average 99 stars, based on 1 article reviews
    gene exp tbp hs00427620 m1 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Identification of hub genes and lncRNAs driving the transition from early response to methotrexate resistance in HT29 colon cancer cells.
    Article Snippet: .. Caveolin-1 (CAV1) mRNA levels were determined by RT-qPCR using TaqMan Gene Expression Assays for CAV1 (Hs00971716_m1) and TBP (Hs00427620_m1) (both probes from Thermofisher Scientific, Madrid, Spain) as the endogenous control, using 150 ng of cDNA per reaction. ..

    Article Title: Divalent siRNA for prion disease
    Article Snippet: .. Reverse transcriptase-polymerase chain reaction samples were prepared using 2 μl or cell lysate, Taqman 1-Step qRT-PCR master mix and Taqman gene expression assays for human TBP (Invitrogen, cat no.: Hs00427620_m1) and human PRNP (Invitrogen, cat no.: Hs00175591_m1) in a 20 μl reaction volume. .. Samples were run on a QuantStudio 7 Flex system (Applied Biosystems) in a MicroAmp Optical 384-well reaction plate (Applied Biosystems, cat no.: 4309849) using the following cycling conditions: reverse transcription 50°C, 5 min; reverse transcription inactivation/initial denaturation 95°C, 20 s; amplification 95°C, 3 s, 60°C, 30 s, 40 cycles.

    Article Title: Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndrome
    Article Snippet: RNA was extracted from lysed cells using NucleoSpin 96 RNA Core Kit (#740466.4; MACHEREY-NAGEL), according to the manufacturer’s instructions and eluted in 75 μl RNase-free H2O. .. Quantitative RT-PCR was performed with TaqMan RNA-to-CT 1-Step Kit (#4392938; Thermo Fisher Scientific) using probes targeting TATA-box binding protein (TBP, #Hs00427620_m1; Thermo Fisher Scientific), CXCL10 (#Hs01124251_g1; Thermo Fisher Scientific), IL-6 (#Hs00985639_m1; Thermo Fisher Scientific), ICAM1 (#Hs00164932_m1; Thermo Fisher Scientific), and IRF7 (#Hs01014809_g1; Thermo Fisher Scientific), according to the manufacturer’s instructions. .. A QuantStudio 3 Real-Time PCR System (Applied Biosystems) was used for quantification of RNA levels relative to the expression of TBP using the 2 −ΔΔCt method , and the expression levels were normalized to the controls.

    Article Title: Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndrome.
    Article Snippet: RNA was extracted from lysed cells using NucleoSpin 96 RNA Core Kit (#740466.4; MACHEREY-NAGEL), according to the manufacturer’s instructions and eluted in 75 μl RNase-free H2O. .. Quantitative RT-PCR was performed with TaqMan RNA-to-CT 1- Step Kit (#4392938; Thermo Fisher Scientific) using probes targeting TATA-box binding protein (TBP, #Hs00427620_m1; Thermo Fisher Scientific), CXCL10 (#Hs01124251_g1; Thermo Fisher Scientific), IL-6 (#Hs00985639_m1; Thermo Fisher Scientific), ICAM1 (#Hs00164932_m1; Thermo Fisher Scientific), and IRF7 (#Hs01014809_g1; Thermo Fisher Scientific), according to the manufacturer’s instructions. .. A QuantStudio 3 Real-Time PCR System (Applied Biosystems) was used for quantification of RNA levels relative to the expression of TBP using the 2−ΔΔCt method (88), and the expression levels were normalized to the controls.

    Gene Expression:

    Article Title: Identification of hub genes and lncRNAs driving the transition from early response to methotrexate resistance in HT29 colon cancer cells.
    Article Snippet: .. Caveolin-1 (CAV1) mRNA levels were determined by RT-qPCR using TaqMan Gene Expression Assays for CAV1 (Hs00971716_m1) and TBP (Hs00427620_m1) (both probes from Thermofisher Scientific, Madrid, Spain) as the endogenous control, using 150 ng of cDNA per reaction. ..

    Article Title: Divalent siRNA for prion disease
    Article Snippet: .. Reverse transcriptase-polymerase chain reaction samples were prepared using 2 μl or cell lysate, Taqman 1-Step qRT-PCR master mix and Taqman gene expression assays for human TBP (Invitrogen, cat no.: Hs00427620_m1) and human PRNP (Invitrogen, cat no.: Hs00175591_m1) in a 20 μl reaction volume. .. Samples were run on a QuantStudio 7 Flex system (Applied Biosystems) in a MicroAmp Optical 384-well reaction plate (Applied Biosystems, cat no.: 4309849) using the following cycling conditions: reverse transcription 50°C, 5 min; reverse transcription inactivation/initial denaturation 95°C, 20 s; amplification 95°C, 3 s, 60°C, 30 s, 40 cycles.

    Control:

    Article Title: Identification of hub genes and lncRNAs driving the transition from early response to methotrexate resistance in HT29 colon cancer cells.
    Article Snippet: .. Caveolin-1 (CAV1) mRNA levels were determined by RT-qPCR using TaqMan Gene Expression Assays for CAV1 (Hs00971716_m1) and TBP (Hs00427620_m1) (both probes from Thermofisher Scientific, Madrid, Spain) as the endogenous control, using 150 ng of cDNA per reaction. ..

    other:

    Article Title: TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory network
    Article Snippet: The following TaqMan probes were used: Tbp - Mm01277042_m1, Tbl1x - Mm01222202_m1, Tbl1xr1 - Mm01283877_m1, Ins1 - Mm01259683_g1, Ins2 - Mm00731595_gH, Nkx6.1 - Mm00454961_m1, Slc2a2 - Mm00454961_m1, Mafa - Mm00845206_s1, Pdx1 - Mm00435565_m1, Pax6 - Mm00443081_m1, Ucn3 - Mm00453206_s1, ChgA - Mm00514341_m1, Ngn3 - Mm00437606_s1, Ldha - Mm01612132_g1, Hk1 - Mm00439344_m1, TBP - Hs00427620_m1, TBL1X - Hs00959540_m1, TBL1XR1 - Hs00226564_m1.

    Article Title: TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory network.
    Article Snippet: The followingTaqMan probes were used: Tbp - Mm01277042_m1, Tbl1x - Mm01222202_m1, Tbl1xr1 - Mm01283877_m1, Ins1 - Mm01259683_g1, Ins2 - Mm00731595_gH, Nkx6.1 - Mm00454961_m1, Slc2a2 - Mm00454961_m1, Mafa - Mm00845206_s1, Pdx1 - Mm00435565_m1, Pax6 - Mm00443081_m1, Ucn3 - Mm00453206_s1, ChgA - Mm00514341_m1,Ngn3 - Mm00437606_s1, Ldha -Mm01612132_g1,Hk1 - Mm00439344_m1, TBP - Hs00427620_m1, TBL1X - Hs00959540_m1, TBL1XR1 - Hs00226564_m1.

    Article Title: CD4 + and CD8 + T cells contribute to psoriasis phenotype when integrated into human skin equivalents for drug testing
    Article Snippet: The following TaqManTM probes were used: IFN-γ (Hs00989291_m1), IL-17A (Hs00174383_m1), IL-17F (Hs00174383_m1), IL-4 (Hs00174122_m1), IL-13 (Hs00174379_m1), IL-22 (Hs01574154_m1) and TBP (Hs00427620_m1).

    Binding Assay:

    Article Title: Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndrome
    Article Snippet: RNA was extracted from lysed cells using NucleoSpin 96 RNA Core Kit (#740466.4; MACHEREY-NAGEL), according to the manufacturer’s instructions and eluted in 75 μl RNase-free H2O. .. Quantitative RT-PCR was performed with TaqMan RNA-to-CT 1-Step Kit (#4392938; Thermo Fisher Scientific) using probes targeting TATA-box binding protein (TBP, #Hs00427620_m1; Thermo Fisher Scientific), CXCL10 (#Hs01124251_g1; Thermo Fisher Scientific), IL-6 (#Hs00985639_m1; Thermo Fisher Scientific), ICAM1 (#Hs00164932_m1; Thermo Fisher Scientific), and IRF7 (#Hs01014809_g1; Thermo Fisher Scientific), according to the manufacturer’s instructions. .. A QuantStudio 3 Real-Time PCR System (Applied Biosystems) was used for quantification of RNA levels relative to the expression of TBP using the 2 −ΔΔCt method , and the expression levels were normalized to the controls.

    Article Title: Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndrome.
    Article Snippet: RNA was extracted from lysed cells using NucleoSpin 96 RNA Core Kit (#740466.4; MACHEREY-NAGEL), according to the manufacturer’s instructions and eluted in 75 μl RNase-free H2O. .. Quantitative RT-PCR was performed with TaqMan RNA-to-CT 1- Step Kit (#4392938; Thermo Fisher Scientific) using probes targeting TATA-box binding protein (TBP, #Hs00427620_m1; Thermo Fisher Scientific), CXCL10 (#Hs01124251_g1; Thermo Fisher Scientific), IL-6 (#Hs00985639_m1; Thermo Fisher Scientific), ICAM1 (#Hs00164932_m1; Thermo Fisher Scientific), and IRF7 (#Hs01014809_g1; Thermo Fisher Scientific), according to the manufacturer’s instructions. .. A QuantStudio 3 Real-Time PCR System (Applied Biosystems) was used for quantification of RNA levels relative to the expression of TBP using the 2−ΔΔCt method (88), and the expression levels were normalized to the controls.



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