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gene array scanner 3000 7g  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene array scanner 3000 7g
    Gene Array Scanner 3000 7g, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+array+scanner/pmc11754234-164-17-16
    Average 90 stars, based on 1 article reviews
    gene array scanner 3000 7g - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Nutritional stress in larvae induces adaptive responses that transcend generations in males of a model insect.
    Article Snippet: Next, 5.5 μg fragmented and labelled sense-strand cDNA was hybridised to Affymetrix Drosophila Gene 1.0 arrays for 16 h at 45°C in a GeneChip Hybridization Oven 640. .. Hybridised arrays were washed and stained in an Affymetrix Fluidics Station FS450, and the fluorescence signals were measured with an Affymetrix GeneChip Scanner 3000 7G. .. Fluidics and scan functions were controlled by the Affymetrix GeneChip Command Console v.4.1.3 software.

    Article Title: Two independent families with de novo whole APC gene deletion and intellectual disability: a case report
    Article Snippet: Briefly, 250 ng of patient DNA was digested with Nsp1, amplified with TITANIUM Taq DNA polymerase, fragmented with the Affymetrix fragmentation reagent (Thermo Fisher Scientific), and labelled with biotin end-labelled nucleotides. .. DNA was hybridised to the microarray for 16 h, washed on an Affymetrix GeneChip Fluidics Station 450 (Thermo Fisher Scientific), stained with Affymetrix GeneChip Stain Reagents, and scanned using a GeneChip Scanner 3000 7G (Thermo Fisher Scientific). .. Data analysis was performed using Chromosome Analysis Suite software version 4.3 (Thermo Fisher Scientific).

    Article Title: Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome.
    Article Snippet: 3,8 μg of fragmented and labeled ss cDNA were hybridized to Applied Biosystems GeneChip Porcine Gene 1.0 ST arrays which covers 19,212 genes represented by 394,580 probes, for 16 h at 45° C and 60 rpm in an Applied Biosystems GeneChip hybridization oven 640. .. Hybridized arrays were washed and stained in an Applied Biosystems GeneChip Fluidics Station FS450, and the fluorescent signals were measured with an Applied Biosystems GeneChip Scanner 3000 7G System. .. Fluidics and scan functions were controlled by the Applied Biosystems GeneChip Command Console v5.0 software.

    Article Title: CD36 enrichment in HER2-positive mesenchymal stem cells drives therapy refractoriness in breast cancer.
    Article Snippet: .. After washing and staining using the GeneChip Fluidics Station 450, the arrays were scanned using an Affymetrix Gene Chip Scanner 3000 7G. .. Primary data were acquired using Affymetrix GeneChip Command Scan Control version 4.0 (developed by Thermo Fisher Scientific).

    Fluorescence:

    Article Title: Nutritional stress in larvae induces adaptive responses that transcend generations in males of a model insect.
    Article Snippet: Next, 5.5 μg fragmented and labelled sense-strand cDNA was hybridised to Affymetrix Drosophila Gene 1.0 arrays for 16 h at 45°C in a GeneChip Hybridization Oven 640. .. Hybridised arrays were washed and stained in an Affymetrix Fluidics Station FS450, and the fluorescence signals were measured with an Affymetrix GeneChip Scanner 3000 7G. .. Fluidics and scan functions were controlled by the Affymetrix GeneChip Command Console v.4.1.3 software.

    Article Title: Distinct mRNA expression profiles and miRNA regulators of the PI3K/AKT/mTOR pathway in breast cancer: insights into tumor progression and therapeutic targets
    Article Snippet: .. The process included cDNA synthesis, RNA amplification, and hybridization, followed by fluorescence intensity measurement with the Affymetrix Gene Array Scanner 3000 7G. .. Microarray analysis of miRNAs involved in the PI3K/AKT/mTOR pathway was performed using the GeneChip miRNA 2.0 Array (Affymetrix, Santa Clara, CA).

    other:

    Article Title: Modulation of biological activities in adipose derived stem cells by histone deacetylation.
    Article Snippet: The cells were incubated with a blocking solution containing 3% BSA for 1 h, followed by overnight incubation with monoclonal rabbit anti-human Ki67/MKI67 (Novus Biologicals Inc, USA); (1:250) at 4 °C, followed by 1 h incubation in the darkness with the secondary polyclonal goat anti-rabbit labeled with Alexa Fluor 488 (Abcam, USA); (1:1000).

    Microarray:

    Article Title: Two independent families with de novo whole APC gene deletion and intellectual disability: a case report
    Article Snippet: Briefly, 250 ng of patient DNA was digested with Nsp1, amplified with TITANIUM Taq DNA polymerase, fragmented with the Affymetrix fragmentation reagent (Thermo Fisher Scientific), and labelled with biotin end-labelled nucleotides. .. DNA was hybridised to the microarray for 16 h, washed on an Affymetrix GeneChip Fluidics Station 450 (Thermo Fisher Scientific), stained with Affymetrix GeneChip Stain Reagents, and scanned using a GeneChip Scanner 3000 7G (Thermo Fisher Scientific). .. Data analysis was performed using Chromosome Analysis Suite software version 4.3 (Thermo Fisher Scientific).

    cDNA Synthesis:

    Article Title: Distinct mRNA expression profiles and miRNA regulators of the PI3K/AKT/mTOR pathway in breast cancer: insights into tumor progression and therapeutic targets
    Article Snippet: .. The process included cDNA synthesis, RNA amplification, and hybridization, followed by fluorescence intensity measurement with the Affymetrix Gene Array Scanner 3000 7G. .. Microarray analysis of miRNAs involved in the PI3K/AKT/mTOR pathway was performed using the GeneChip miRNA 2.0 Array (Affymetrix, Santa Clara, CA).

    RNA Amplification:

    Article Title: Distinct mRNA expression profiles and miRNA regulators of the PI3K/AKT/mTOR pathway in breast cancer: insights into tumor progression and therapeutic targets
    Article Snippet: .. The process included cDNA synthesis, RNA amplification, and hybridization, followed by fluorescence intensity measurement with the Affymetrix Gene Array Scanner 3000 7G. .. Microarray analysis of miRNAs involved in the PI3K/AKT/mTOR pathway was performed using the GeneChip miRNA 2.0 Array (Affymetrix, Santa Clara, CA).

    Hybridization:

    Article Title: Distinct mRNA expression profiles and miRNA regulators of the PI3K/AKT/mTOR pathway in breast cancer: insights into tumor progression and therapeutic targets
    Article Snippet: .. The process included cDNA synthesis, RNA amplification, and hybridization, followed by fluorescence intensity measurement with the Affymetrix Gene Array Scanner 3000 7G. .. Microarray analysis of miRNAs involved in the PI3K/AKT/mTOR pathway was performed using the GeneChip miRNA 2.0 Array (Affymetrix, Santa Clara, CA).



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