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recombinant dna crispr cas9 plasmid lenticrisprv2  (Addgene inc)


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    Structured Review

    Addgene inc recombinant dna crispr cas9 plasmid lenticrisprv2
    Recombinant Dna Crispr Cas9 Plasmid Lenticrisprv2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ge+array+expression+analysis+suite+software/lentiCRISPR+v2+(Plasmid+%2352961)/pm39303720-258-253-263
    Average 96 stars, based on 6125 article reviews
    recombinant dna crispr cas9 plasmid lenticrisprv2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Knock-Out:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Clone Assay:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: HER2 heterogeneous breast cancer models reveal novel therapeutic targets and subclonal dynamics during evolution to resistance to HER2-targeted therapies
    Article Snippet: .. Guides were cloned in lentiCRISPRv2 (Addgene #52961) following 935 the recommended protocol (65). ..

    Article Title: ELF3 drives glioblastoma multiforme progression through the regulation of MXRA8 expression.
    Article Snippet: Single-guide RNAs (sgRNAs) targeting ELF3 and MXRA8 were designed using the online CRISPR tool (http://crispr.mit.edu) and synthesized by GenePharma (Shanghai, China). .. The sgRNA sequences were cloned into the lentiCRISPR v2 plasmid (Addgene, #52961). .. Lentivirus production was achieved by co-transfecting HEK293T cells with the lentiCRISPR v2 plasmid, psPAX2 packaging plasmid (Addgene, #12260), and pMD2.G envelope plasmid (Addgene, #12259), facilitated by Lipofectamine 3000.

    Plasmid Preparation:

    Article Title: Oxymatrine attenuates melanoma progression and metastasis through SORBS2-mediated suppression of M2 macrophage polarization
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Hypofucosylation promotes pertussis toxin binding to cell surface glycococonjugates and pertussis toxin-induced intracellular ERK signaling
    Article Snippet: .. Predesigned CRISPR sgRNAs were ligated into the lentiCRISPR-v2 vector (Addgene, plasmid no. 52961). ..

    Article Title: ELF3 drives glioblastoma multiforme progression through the regulation of MXRA8 expression.
    Article Snippet: Single-guide RNAs (sgRNAs) targeting ELF3 and MXRA8 were designed using the online CRISPR tool (http://crispr.mit.edu) and synthesized by GenePharma (Shanghai, China). .. The sgRNA sequences were cloned into the lentiCRISPR v2 plasmid (Addgene, #52961). .. Lentivirus production was achieved by co-transfecting HEK293T cells with the lentiCRISPR v2 plasmid, psPAX2 packaging plasmid (Addgene, #12260), and pMD2.G envelope plasmid (Addgene, #12259), facilitated by Lipofectamine 3000.

    Article Title: Inhibition of circulating glycocholic acid-regulated signaling potentiates immune checkpoint therapy in colorectal cancer.
    Article Snippet: Short hairpin RNA (shRNA) sequences targeting SOX14 and DHHC9 were cloned into the pLKO.1 vector (cat. no. 10878; Addgene, Inc.), and the primers used are listed in Supplementary Data 5. .. Single guide RNAs (sgRNAs) targeting FXR or CD274 were constructed into the lentiCRISPR v2 vector (cat. no. 52961; Addgene, Inc.). ..

    Article Title: GABA agonists and antagonists affect differentiation of hematopoietic stem cells and megakaryocyte progenitors
    Article Snippet: .. The LentiCRISPR V2 plasmid was purchase from Addgene. ..

    CRISPR:

    Article Title: Hypofucosylation promotes pertussis toxin binding to cell surface glycococonjugates and pertussis toxin-induced intracellular ERK signaling
    Article Snippet: .. Predesigned CRISPR sgRNAs were ligated into the lentiCRISPR-v2 vector (Addgene, plasmid no. 52961). ..

    Software:

    Article Title: An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pCAGGS-FLAG-DCAF16 This paper N/A pCAGGS-FLAG-DCAF17 This paper N/A pEU-E01-FLAG-GST-MCS Yamanaka et al.120 N/A pEU-E01-bls-MCS Yamanaka et al.120 N/A pEU-E01-FLAG-GST-DDB1 This paper N/A pEU-E01-bls-DCAF This paper N/A lentiCRISPRv2 Addgene Cat# 52961 lentiCRISPRv2-COP1 This paper N/A lentiCRISPRv2-DCAF3 This paper N/A Software and algorithms GraphPad Prism 10 software GraphPad RRID: SCR_002798 ImageJ Fiji software NIH https://imagej.net/software/fiji/downloads Rstudio software RStudio, Inc. https://posit.co/download/rstudio- desktop/ Cytoscape software NHGRI https://cytoscape.org/index.html Proteome Discoverer software Thermo Fisher Scientific RRID: SCR_014477 DIA-NN 1.9 Demichev et al.128 https://github.com/vdemichev/DiaNN Perseus Cox Lab, Max Planck Institute of Biochemistry SCR_015753 StrandNGS v4.0 Strand Life Sciences Pvt Ltd N/A mord version 0.3 Pedregosa et al.129 https://pypi.org/project/mord/ PVclust Suzuki & Shimoda130 https://github.com/shimo-lab/pvclust NetworkX Hagberg et al.131 https://networkx.org/documentation/ stable/index.html ImageQuant LAS 4000 GE Healthcare N/A LightCycler 96 software Roche N/A Microsoft® Excel for Mac 2024 Microsoft N/A Other DMEM Low-glucose FUJIFILM Wako Pure Chemical Corporation Cat# 041-29775 FBS FUJIFILM Wako Pure Chemical Corporation Cat# 553-36315 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 TrypLE Select enzyme Thermo Fisher Scientific Cat# 12563029 Opti-MEM Thermo Fisher Scientific Cat# 11058021 PEI Max Polysciences Cat# 24765-1 siLentFect Lipid Reagent for RNAi BIO-RAD Cat# 1703361 Immobilon®-P PVDF Membrane Millipore Cat# IPVH00010 Lenti-XTM Concentrator Takara Bio Cat# Z1232N Lentiviral High Titer Packaging Mix Takara Bio Cat# 6194 e4 Molecular Cell 86, 1397–1416.e1–e11, April 2, 2026 medium containing lentivirus particles was collected, filtered, and enriched using a Lenti-XTM Concentrator. ..

    Data-independent acquisition:

    Article Title: An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pCAGGS-FLAG-DCAF16 This paper N/A pCAGGS-FLAG-DCAF17 This paper N/A pEU-E01-FLAG-GST-MCS Yamanaka et al.120 N/A pEU-E01-bls-MCS Yamanaka et al.120 N/A pEU-E01-FLAG-GST-DDB1 This paper N/A pEU-E01-bls-DCAF This paper N/A lentiCRISPRv2 Addgene Cat# 52961 lentiCRISPRv2-COP1 This paper N/A lentiCRISPRv2-DCAF3 This paper N/A Software and algorithms GraphPad Prism 10 software GraphPad RRID: SCR_002798 ImageJ Fiji software NIH https://imagej.net/software/fiji/downloads Rstudio software RStudio, Inc. https://posit.co/download/rstudio- desktop/ Cytoscape software NHGRI https://cytoscape.org/index.html Proteome Discoverer software Thermo Fisher Scientific RRID: SCR_014477 DIA-NN 1.9 Demichev et al.128 https://github.com/vdemichev/DiaNN Perseus Cox Lab, Max Planck Institute of Biochemistry SCR_015753 StrandNGS v4.0 Strand Life Sciences Pvt Ltd N/A mord version 0.3 Pedregosa et al.129 https://pypi.org/project/mord/ PVclust Suzuki & Shimoda130 https://github.com/shimo-lab/pvclust NetworkX Hagberg et al.131 https://networkx.org/documentation/ stable/index.html ImageQuant LAS 4000 GE Healthcare N/A LightCycler 96 software Roche N/A Microsoft® Excel for Mac 2024 Microsoft N/A Other DMEM Low-glucose FUJIFILM Wako Pure Chemical Corporation Cat# 041-29775 FBS FUJIFILM Wako Pure Chemical Corporation Cat# 553-36315 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 TrypLE Select enzyme Thermo Fisher Scientific Cat# 12563029 Opti-MEM Thermo Fisher Scientific Cat# 11058021 PEI Max Polysciences Cat# 24765-1 siLentFect Lipid Reagent for RNAi BIO-RAD Cat# 1703361 Immobilon®-P PVDF Membrane Millipore Cat# IPVH00010 Lenti-XTM Concentrator Takara Bio Cat# Z1232N Lentiviral High Titer Packaging Mix Takara Bio Cat# 6194 e4 Molecular Cell 86, 1397–1416.e1–e11, April 2, 2026 medium containing lentivirus particles was collected, filtered, and enriched using a Lenti-XTM Concentrator. ..

    Membrane:

    Article Title: An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pCAGGS-FLAG-DCAF16 This paper N/A pCAGGS-FLAG-DCAF17 This paper N/A pEU-E01-FLAG-GST-MCS Yamanaka et al.120 N/A pEU-E01-bls-MCS Yamanaka et al.120 N/A pEU-E01-FLAG-GST-DDB1 This paper N/A pEU-E01-bls-DCAF This paper N/A lentiCRISPRv2 Addgene Cat# 52961 lentiCRISPRv2-COP1 This paper N/A lentiCRISPRv2-DCAF3 This paper N/A Software and algorithms GraphPad Prism 10 software GraphPad RRID: SCR_002798 ImageJ Fiji software NIH https://imagej.net/software/fiji/downloads Rstudio software RStudio, Inc. https://posit.co/download/rstudio- desktop/ Cytoscape software NHGRI https://cytoscape.org/index.html Proteome Discoverer software Thermo Fisher Scientific RRID: SCR_014477 DIA-NN 1.9 Demichev et al.128 https://github.com/vdemichev/DiaNN Perseus Cox Lab, Max Planck Institute of Biochemistry SCR_015753 StrandNGS v4.0 Strand Life Sciences Pvt Ltd N/A mord version 0.3 Pedregosa et al.129 https://pypi.org/project/mord/ PVclust Suzuki & Shimoda130 https://github.com/shimo-lab/pvclust NetworkX Hagberg et al.131 https://networkx.org/documentation/ stable/index.html ImageQuant LAS 4000 GE Healthcare N/A LightCycler 96 software Roche N/A Microsoft® Excel for Mac 2024 Microsoft N/A Other DMEM Low-glucose FUJIFILM Wako Pure Chemical Corporation Cat# 041-29775 FBS FUJIFILM Wako Pure Chemical Corporation Cat# 553-36315 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 TrypLE Select enzyme Thermo Fisher Scientific Cat# 12563029 Opti-MEM Thermo Fisher Scientific Cat# 11058021 PEI Max Polysciences Cat# 24765-1 siLentFect Lipid Reagent for RNAi BIO-RAD Cat# 1703361 Immobilon®-P PVDF Membrane Millipore Cat# IPVH00010 Lenti-XTM Concentrator Takara Bio Cat# Z1232N Lentiviral High Titer Packaging Mix Takara Bio Cat# 6194 e4 Molecular Cell 86, 1397–1416.e1–e11, April 2, 2026 medium containing lentivirus particles was collected, filtered, and enriched using a Lenti-XTM Concentrator. ..

    Construct:

    Article Title: Inhibition of circulating glycocholic acid-regulated signaling potentiates immune checkpoint therapy in colorectal cancer.
    Article Snippet: Short hairpin RNA (shRNA) sequences targeting SOX14 and DHHC9 were cloned into the pLKO.1 vector (cat. no. 10878; Addgene, Inc.), and the primers used are listed in Supplementary Data 5. .. Single guide RNAs (sgRNAs) targeting FXR or CD274 were constructed into the lentiCRISPR v2 vector (cat. no. 52961; Addgene, Inc.). ..



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