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gcamp6s  (Micromass UK Limited)

 
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    Structured Review

    Micromass UK Limited gcamp6s
    Ca2+ transients in limb mesenchyme are regulated by voltage-gated L-type Ca2+ channels. (A–O) Live-imaging analyses of Ca2+ transients visualized with <t>GCaMP6s</t> in micromass-cultured chicken limb bud cells. mCherry was co-overexpressed as a control signal. The micromass-cultured cells were treated with K-gluconate (40 mM; C and D), Nifedipine (250 μM; H and I) or both (M and N). At each time point, images were thresholded to visualize GCaMP transients and newly emerged GCaMP signals were counted as GCaMP transients, as marked by yellow arrowheads in (B′, D′, G′, I′, L′, and N′). (E, J, and O) Temporal dynamics of GCaMP transients (n = 3 each). Time-lapse analyses started 5 h after plating. GCaMP flashes were counted every minute for 60 min. (Scale bars: 100 μm in A, F, and K.)
    Gcamp6s, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp+visualization/gcamp6s/pmc06815189-79-75-77
    Average 90 stars, based on 1 article reviews
    gcamp6s - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "L-type voltage-gated Ca 2+ channel Ca V 1.2 regulates chondrogenesis during limb development"

    Article Title: L-type voltage-gated Ca 2+ channel Ca V 1.2 regulates chondrogenesis during limb development

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1908981116

    Ca2+ transients in limb mesenchyme are regulated by voltage-gated L-type Ca2+ channels. (A–O) Live-imaging analyses of Ca2+ transients visualized with GCaMP6s in micromass-cultured chicken limb bud cells. mCherry was co-overexpressed as a control signal. The micromass-cultured cells were treated with K-gluconate (40 mM; C and D), Nifedipine (250 μM; H and I) or both (M and N). At each time point, images were thresholded to visualize GCaMP transients and newly emerged GCaMP signals were counted as GCaMP transients, as marked by yellow arrowheads in (B′, D′, G′, I′, L′, and N′). (E, J, and O) Temporal dynamics of GCaMP transients (n = 3 each). Time-lapse analyses started 5 h after plating. GCaMP flashes were counted every minute for 60 min. (Scale bars: 100 μm in A, F, and K.)
    Figure Legend Snippet: Ca2+ transients in limb mesenchyme are regulated by voltage-gated L-type Ca2+ channels. (A–O) Live-imaging analyses of Ca2+ transients visualized with GCaMP6s in micromass-cultured chicken limb bud cells. mCherry was co-overexpressed as a control signal. The micromass-cultured cells were treated with K-gluconate (40 mM; C and D), Nifedipine (250 μM; H and I) or both (M and N). At each time point, images were thresholded to visualize GCaMP transients and newly emerged GCaMP signals were counted as GCaMP transients, as marked by yellow arrowheads in (B′, D′, G′, I′, L′, and N′). (E, J, and O) Temporal dynamics of GCaMP transients (n = 3 each). Time-lapse analyses started 5 h after plating. GCaMP flashes were counted every minute for 60 min. (Scale bars: 100 μm in A, F, and K.)

    Techniques Used: Imaging, Cell Culture, Control

    Related Articles

    Control:

    Article Title: L-type voltage-gated Ca 2+ channel Ca V 1.2 regulates chondrogenesis during limb development
    Article Snippet: .. This resulted in an increase in frequency of the Ca 2+ transients to 5.27% ( P < 0.01 vs. control), implying that VGCCs are activated in response to depolarization ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 2. caption a7 Ca 2+ transients in limb mesenchyme are regulated by voltage-gated L-type Ca 2+ channels. ( A – O ) Live-imaging analyses of Ca 2+ transients visualized with GCaMP6s in micromass-cultured chicken limb bud cells. mCherry was co-overexpressed as a control signal. .. Micromass cultures were established from transgenic mice carrying a Col2a1 -tdTomato reporter that produces a red fluorescent signal in chondrocytes, the cell type that expresses the endogenous Col2al gene.



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