sv40pa n500r phusion high fidelity pcr master mix (New England Biolabs)
New England Biolabs is a verified supplier 
New England Biolabs manufactures this product 
99
Structured Review
New England Biolabs
sv40pa n500r phusion high fidelity pcr master mix
Sv40pa N500r Phusion High Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gc+buffer/Phusion+High-Fidelity+PCR+Master+Mix+with+GC+Buffer/pmc06054316-615-25-34
Average 99 stars, based on 805 article reviews
Sv40pa N500r Phusion High Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gc+buffer/Phusion+High-Fidelity+PCR+Master+Mix+with+GC+Buffer/pmc06054316-615-25-34
Average 99 stars, based on 805 article reviews
sv40pa n500r phusion high fidelity pcr master mix - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "In vivo and In vitro methods to identify DNA sequence variants that alter RNA Splicing"
Article Title: In vivo and In vitro methods to identify DNA sequence variants that alter RNA Splicing
Journal: Current protocols in human genetics
doi: 10.1002/cphg.60
Figure Legend Snippet: In PCR #1, a 500 bp test sequence and 225 bp SV40PA sequence are added to the same reaction to form a test sequence-SV40PA combined construct. In PCR #2, the PCR #1 product and a 600 bp CMV Promoter are added to the same reaction to form the full minigene construct. Overlapping, homologous regions of these gene fragments allow for assembly to occur.
Techniques Used: Sequencing, Construct
Figure Legend Snippet: gBlocks and other primers used in laboratory protocols
Techniques Used: Sequencing, Multiplexing, Amplification
Figure Legend Snippet: PCR #1 set up. Repeat for as many gBlocks as are required.
Techniques Used: Concentration Assay, Sequencing, Construct
Figure Legend Snippet: 15 lanes containing independent PCR #1 products are shown. Each band is approximately 650-700 bp in size, and represents an individual test sequence (500 bp) attached to a SV40PA signal (225 bp). Note that products seem to be migrating to the same positions in pairs, as would be expected with reference and alternate containing test sequences that have identical lengths.
Techniques Used: Sequencing
Figure Legend Snippet: PCR #2 set up. Repeat for as many gBlocks as are required.
Techniques Used: Concentration Assay
Figure Legend Snippet: Amplification of CMVp-N500
Techniques Used: Amplification, Concentration Assay, Sequencing
Related Articles
Amplification:Article Title: How Bacterial Growth-Phase Reshapes Synthetic Gene Circuit Function Article Snippet: DNA oligos and genes were ordered using the Oligo Rapid Service from Azenta Life Sciences. .. DNA fragments to be assembled were amplified by PCR using Article Title: Yupingfeng powder attenuates influenza A virus induced lung injury via gut microbiota-dependent modulation of branched-chain amino acid metabolism and macrophage polarization Article Snippet: .. PCR amplification was performed using barcode-specific primers, Article Title: Analysis of rumen fermentation, bacterial diversity and blood biochemical parameters in sheep fed with cotton straw and different roughage combinations Article Snippet: .. The polymerase chain reaction (PCR) amplification used specific barcoded primers: forward primer 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′) with the Article Title: Comparative Analysis of the EF-1α Intergenic Region in Babesia divergens Isolates: Insights into TA Repeat Variation and Potential Regulatory Implications Article Snippet: All primers were designed using the Primer QuestTM Tool ( https://www.idtdna.com/pages/tools/primerquest , accessed on 9 May 2023) ( ). .. PCR amplification was carried out using Phusion ® High-Fidelity PCR Master Mix with Article Title: Soil Fertility Differences and Bacterial and Fungal Community Variation Among Subtropical Forest Stands Dominated by Different Tree Species Article Snippet: .. PCR amplification was performed using barcode-tagged primers and Phusion HighFidelity PCR Master Mix with Article Title: Comparative Analysis of the EF-1α Intergenic Region in Babesia divergens Isolates: Insights into TA Repeat Variation and Potential Regulatory Implications. Article Snippet: All primers were designed using the Primer QuestTM Tool (https://www.idtdna.com/ pages/tools/primerquest, accessed on 9 May 2023) (Table S1 and Figure S4). .. PCR amplification was carried out using Phusion® High-Fidelity PCR Master Mix with Polymerase Chain Reaction:Article Title: How Bacterial Growth-Phase Reshapes Synthetic Gene Circuit Function Article Snippet: DNA oligos and genes were ordered using the Oligo Rapid Service from Azenta Life Sciences. .. DNA fragments to be assembled were amplified by PCR using Article Title: Yupingfeng powder attenuates influenza A virus induced lung injury via gut microbiota-dependent modulation of branched-chain amino acid metabolism and macrophage polarization Article Snippet: .. PCR amplification was performed using barcode-specific primers, Article Title: Analysis of rumen fermentation, bacterial diversity and blood biochemical parameters in sheep fed with cotton straw and different roughage combinations Article Snippet: .. The polymerase chain reaction (PCR) amplification used specific barcoded primers: forward primer 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′) with the Article Title: Comparative Analysis of the EF-1α Intergenic Region in Babesia divergens Isolates: Insights into TA Repeat Variation and Potential Regulatory Implications Article Snippet: All primers were designed using the Primer QuestTM Tool ( https://www.idtdna.com/pages/tools/primerquest , accessed on 9 May 2023) ( ). .. PCR amplification was carried out using Phusion ® High-Fidelity PCR Master Mix with Article Title: Soil Fertility Differences and Bacterial and Fungal Community Variation Among Subtropical Forest Stands Dominated by Different Tree Species Article Snippet: .. PCR amplification was performed using barcode-tagged primers and Phusion HighFidelity PCR Master Mix with Article Title: Taxonomic shifts in bacterial communities of mine tailings suggest persistence of chemolithotrophic traits under oligotrophic and metal-rich conditions Article Snippet: .. PCR reactions were performed with Article Title: Comparative Analysis of the EF-1α Intergenic Region in Babesia divergens Isolates: Insights into TA Repeat Variation and Potential Regulatory Implications. Article Snippet: All primers were designed using the Primer QuestTM Tool (https://www.idtdna.com/ pages/tools/primerquest, accessed on 9 May 2023) (Table S1 and Figure S4). .. PCR amplification was carried out using Phusion® High-Fidelity PCR Master Mix with Gas Chromatography:Article Title: How Bacterial Growth-Phase Reshapes Synthetic Gene Circuit Function Article Snippet: DNA oligos and genes were ordered using the Oligo Rapid Service from Azenta Life Sciences. .. DNA fragments to be assembled were amplified by PCR using Article Title: Yupingfeng powder attenuates influenza A virus induced lung injury via gut microbiota-dependent modulation of branched-chain amino acid metabolism and macrophage polarization Article Snippet: .. PCR amplification was performed using barcode-specific primers, Article Title: Analysis of rumen fermentation, bacterial diversity and blood biochemical parameters in sheep fed with cotton straw and different roughage combinations Article Snippet: .. The polymerase chain reaction (PCR) amplification used specific barcoded primers: forward primer 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and reverse primer 806R (5′-GGACTACHVGGGTWTCTAAT-3′) with the Article Title: Soil Fertility Differences and Bacterial and Fungal Community Variation Among Subtropical Forest Stands Dominated by Different Tree Species Article Snippet: .. PCR amplification was performed using barcode-tagged primers and Phusion HighFidelity PCR Master Mix with Article Title: Taxonomic shifts in bacterial communities of mine tailings suggest persistence of chemolithotrophic traits under oligotrophic and metal-rich conditions Article Snippet: .. PCR reactions were performed with Purification:Article Title: Taxonomic shifts in bacterial communities of mine tailings suggest persistence of chemolithotrophic traits under oligotrophic and metal-rich conditions Article Snippet: .. PCR reactions were performed with Gel Extraction:Article Title: Taxonomic shifts in bacterial communities of mine tailings suggest persistence of chemolithotrophic traits under oligotrophic and metal-rich conditions Article Snippet: .. PCR reactions were performed with |