Review



gc buffer  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    New England Biolabs gc buffer
    Gc Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+buffer/Phusion+HF+PCR+MM+HF+Buff/pm41895269-340-8-10
    Average 99 stars, based on 6470 article reviews
    gc buffer - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Flavor development in vacuum dry-salted cowpeas: changes in volatile compounds and non-volatile metabolites during fermentation
    Article Snippet: .. PCR amplification was conducted with Phusion High-Fidelity PCR Master Mix (New England Biolabs). ..

    Article Title: A cold-adapted Cas9 nuclease from the deep ocean
    Article Snippet: Following incubation for 1 h at room temperature, 10μl of the ligation reaction was amplified using Q5 DNA polymerase (New England Biolabs; 15 cycles) with primers specific to the PAM-proximal region of the library and the ligated adapter (Supplementary Data 3). .. PCR products were purified, and Illumina sequencing adapters and sample indices were incorporated through two successive rounds of PCR using Phusion High-Fidelity PCR Master Mix in HF buffer (New England Biolabs; 10 cycles per round). .. Libraries were sequenced on an Illumina MiSeq instrument with a 25% (v/v) PhiX Control v3 spike-in.

    Article Title: Chicken Manure Application Alters Soil Heavy-Metal Bioavailability and Bacterial Community Structure in Pakchoi Fields
    Article Snippet: Chicken manure is widely used in vegetable production, yet its concurrent effects on soil nutrient status, heavy-metal behavior, and soil bacterial communities remain insufficiently understood.. A field experiment was conducted to evaluate five chicken-manure application rates (0, 7.5, 15, 30, and 60 t ha−1) in a pakchoi production system.. Bacterial communities were characterized by 16S rRNA gene sequencing.

    Article Title: Extracellular Vesicle-Encapsulated Small RNAs From Clostridium Butyricum Modulate Host Intestinal Lipid Metabolism.
    Article Snippet: .. CR reactions were performed with Phusion High-Fidelity PCR aster Mix (New England Biolabs, Shanghai, China). .. Sequencing ibraries were constructed using the NEB Next Ultra II FS NA PCR-free Library Prep Kit (New England Biolabs).

    Article Title: Targeted Gastrointestinal Delivery of mRNA Lipid Nanoparticles Enables Systemic Vaccination and Glucagon-like Peptide-1 Metabolic Therapy
    Article Snippet: .. Linear DNA templates for IVT were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), forward primer, and a T120-containing reverse primer. .. The amplified PCR products were purified with the DNA Clean & Concentrator Kit (Zymo Research).

    Article Title: Pediococcus acidilactici PA53 Supplementation Improves Stool Form and Modulates Gut Microbiota and Inflammatory Responses in Adults With Functional Constipation: A Randomized, Double‐Blind, Placebo‐Controlled Trial
    Article Snippet: .. PCR amplification (30 μL reactions) contained 15 μL of Phusion High‐Fidelity PCR Master Mix (New England Biolabs, USA), 0.2 μM of each primer and 10 ng of template DNA. .. Thermal cycles: initial denaturation at 98°C for 1 min; 30 cycles of denaturation at 98°C for 10 s, annealing at 50°C for 30 s, extension at 72°C for 30 s; final extension at 72°C for 5 min. Amplicons were run on 2% agarose gels, purified using the Qiagen Gel Extraction Kit and quantified using a Qubit 2.0 fluorometer.

    Amplification:

    Article Title: Flavor development in vacuum dry-salted cowpeas: changes in volatile compounds and non-volatile metabolites during fermentation
    Article Snippet: .. PCR amplification was conducted with Phusion High-Fidelity PCR Master Mix (New England Biolabs). ..

    Article Title: Gut mycobiome in the spectrum of Metabolic-dysfunction Associated Steatotic Liver Disease (MASLD): from MASH to hepatocellular carcinoma
    Article Snippet: DNA integrity was assessed by agarose gel electrophoresis, and concentrations were measured fluorometrically using a Qubit 2.0 (Life Technologies, USA). .. The V9 region of the 18S rRNA gene was amplified using primers 1380F (5′-CCCTGCCHTTTGTACACAC-3′) and 1510R (5′-CCTTCYGCAGGTTCACCTAC-3′) (Amaral-Zettler et al., 2009) with Phusion High-Fidelity Master Mix (New England Biolabs). ..

    Article Title: Targeted Gastrointestinal Delivery of mRNA Lipid Nanoparticles Enables Systemic Vaccination and Glucagon-like Peptide-1 Metabolic Therapy
    Article Snippet: .. Linear DNA templates for IVT were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), forward primer, and a T120-containing reverse primer. .. The amplified PCR products were purified with the DNA Clean & Concentrator Kit (Zymo Research).

    Article Title: Pediococcus acidilactici PA53 Supplementation Improves Stool Form and Modulates Gut Microbiota and Inflammatory Responses in Adults With Functional Constipation: A Randomized, Double‐Blind, Placebo‐Controlled Trial
    Article Snippet: .. PCR amplification (30 μL reactions) contained 15 μL of Phusion High‐Fidelity PCR Master Mix (New England Biolabs, USA), 0.2 μM of each primer and 10 ng of template DNA. .. Thermal cycles: initial denaturation at 98°C for 1 min; 30 cycles of denaturation at 98°C for 10 s, annealing at 50°C for 30 s, extension at 72°C for 30 s; final extension at 72°C for 5 min. Amplicons were run on 2% agarose gels, purified using the Qiagen Gel Extraction Kit and quantified using a Qubit 2.0 fluorometer.

    Purification:

    Article Title: A cold-adapted Cas9 nuclease from the deep ocean
    Article Snippet: Following incubation for 1 h at room temperature, 10μl of the ligation reaction was amplified using Q5 DNA polymerase (New England Biolabs; 15 cycles) with primers specific to the PAM-proximal region of the library and the ligated adapter (Supplementary Data 3). .. PCR products were purified, and Illumina sequencing adapters and sample indices were incorporated through two successive rounds of PCR using Phusion High-Fidelity PCR Master Mix in HF buffer (New England Biolabs; 10 cycles per round). .. Libraries were sequenced on an Illumina MiSeq instrument with a 25% (v/v) PhiX Control v3 spike-in.



    Similar Products

    93
    New England Biolabs phusion gc buffer
    Phusion Gc Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+buffer/Phusion+GC+Buffer+Pack/pmc13655600-25-39-54
    Average 93 stars, based on 1 article reviews
    phusion gc buffer - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    95
    TaKaRa la taq dna polymerase
    La Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+buffer/TaKaRa+LA+Taq+DNA+Polymerase+with+GC+Buffer/pm42810345-381-6-5
    Average 95 stars, based on 1 article reviews
    la taq dna polymerase - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    99
    New England Biolabs phusion high fidelity pcr master mix
    Phusion High Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+buffer/Phusion+High-Fidelity+PCR+Master+Mix+with+GC+Buffer/bio_rxiv__64898__2026__10__09__752898-166-10-18
    Average 99 stars, based on 1 article reviews
    phusion high fidelity pcr master mix - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs gc buffer
    Gc Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+buffer/Phusion+High-Fidelity+PCR+Master+Mix+with+GC+Buffer/10__3390_slash_f17080994-93-14-16
    Average 99 stars, based on 1 article reviews
    gc buffer - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs q5 high gc enhancer
    Q5 High Gc Enhancer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+buffer/Q5+Reaction+Buffer/pmc13076415-74-17-38
    Average 99 stars, based on 1 article reviews
    q5 high gc enhancer - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    96
    New England Biolabs phusion gc reaction buffer
    Phusion Gc Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+buffer/Phusion+HF+Buffer/pm41861017-373-11-15
    Average 96 stars, based on 1 article reviews
    phusion gc reaction buffer - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    Image Search Results