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af3535  (R&D Systems)


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    Structured Review

    R&D Systems af3535
    Af3535, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/galectin-9/Mouse+Galectin-9+Antibody/us10837966-451-3-6
    Average 93 stars, based on 10 article reviews
    af3535 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Spatially resolved transcriptomics identifies intercellular signaling post-ischemic stroke that controls neural stem cell proliferation
    Article Snippet: .. The primary antibodies used for immunohistochemistry (IHC) in this study included: goat anti-mouse galectin-9 (R&D Systems, Cat. #: AF3535, RRID: AB_2137240) reconstituted at 0.2 mg/ml in sterile PBS and diluted at 1:30; mouse anti-FOXJ1 (eBioscienceTM, Cat. #: 14-9965-80, RRID: AB_1548836) at 1:200 dilution; biotin anti-mouse CD366 (Tim-3) (BioLegend, Cat. #: 119720, RRID: AB_2571936) at 1:150 dilution; rabbit anti-TLR4 (Proteintech, Cat. #: 19811-1-AP, RRID: AB_10638446) at 1:200 dilution; rabbit anti-SOX2 (Cell Signalling Technology, Cat. #: 3728S, RRID: AB_2194037) at 1:250 dilution; goat anti-SOX2 (R&D Systems, Cat. #: AF2018, RRID: AB_355110) reconstituted at 1 mg/ml in sterile PBS and diluted at 1:100; mouse anti-GFAP (Cell Signalling Technology, Cat. #: 3670S, RRID: AB_561049) at 1:500 dilution; chicken anti-GFAP (MilliporeSigma, Sigma-Aldrich, Cat. #: AB5541, RRID: AB_177521) at 1:500 dilution; mouse anti-Ki-67 (BD Biosciences, Cat. #: 550609, RRID: AB_393778) at 1:100 dilution. .. The secondary antibodies used for IHC in this study included: Alexa Fluor ® 488-conjugated donkey anti-chicken secondary antibody (Jackson ImmunoResearch Laboratories, Cat. #: 703-545-155, RRID: AB_2340375) at 1:500 dilution; Alexa Fluor ® 647-conjugated donkey anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories, Cat. #: 711-605-152, RRID: RRID: AB_2492288) at 1:500 dilution; CyTM3-conjugated donkey anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories, Cat. #: 715-165-150, RRID: AB_2340813) at 1:500 dilution; Alexa Fluor ® 488-conjugated donkey anti-goat secondary antibody (Jackson ImmunoResearch Laboratories, Cat. #: 705-545-147, RRID: AB_2336933) at 1:500 dilution; CyTM3 Streptavidin (Jackson ImmunoResearch Laboratories, Cat. #: 016-160-084, RRID: AB_2337244) at 1:1000 dilution.

    Sterility:

    Article Title: Spatially resolved transcriptomics identifies intercellular signaling post-ischemic stroke that controls neural stem cell proliferation
    Article Snippet: .. The primary antibodies used for immunohistochemistry (IHC) in this study included: goat anti-mouse galectin-9 (R&D Systems, Cat. #: AF3535, RRID: AB_2137240) reconstituted at 0.2 mg/ml in sterile PBS and diluted at 1:30; mouse anti-FOXJ1 (eBioscienceTM, Cat. #: 14-9965-80, RRID: AB_1548836) at 1:200 dilution; biotin anti-mouse CD366 (Tim-3) (BioLegend, Cat. #: 119720, RRID: AB_2571936) at 1:150 dilution; rabbit anti-TLR4 (Proteintech, Cat. #: 19811-1-AP, RRID: AB_10638446) at 1:200 dilution; rabbit anti-SOX2 (Cell Signalling Technology, Cat. #: 3728S, RRID: AB_2194037) at 1:250 dilution; goat anti-SOX2 (R&D Systems, Cat. #: AF2018, RRID: AB_355110) reconstituted at 1 mg/ml in sterile PBS and diluted at 1:100; mouse anti-GFAP (Cell Signalling Technology, Cat. #: 3670S, RRID: AB_561049) at 1:500 dilution; chicken anti-GFAP (MilliporeSigma, Sigma-Aldrich, Cat. #: AB5541, RRID: AB_177521) at 1:500 dilution; mouse anti-Ki-67 (BD Biosciences, Cat. #: 550609, RRID: AB_393778) at 1:100 dilution. .. The secondary antibodies used for IHC in this study included: Alexa Fluor ® 488-conjugated donkey anti-chicken secondary antibody (Jackson ImmunoResearch Laboratories, Cat. #: 703-545-155, RRID: AB_2340375) at 1:500 dilution; Alexa Fluor ® 647-conjugated donkey anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories, Cat. #: 711-605-152, RRID: RRID: AB_2492288) at 1:500 dilution; CyTM3-conjugated donkey anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories, Cat. #: 715-165-150, RRID: AB_2340813) at 1:500 dilution; Alexa Fluor ® 488-conjugated donkey anti-goat secondary antibody (Jackson ImmunoResearch Laboratories, Cat. #: 705-545-147, RRID: AB_2336933) at 1:500 dilution; CyTM3 Streptavidin (Jackson ImmunoResearch Laboratories, Cat. #: 016-160-084, RRID: AB_2337244) at 1:1000 dilution.

    other:

    Article Title: Anti-galectin antibody biomarkers predictive of anti-immune checkpoint and anti-angiogenesis responses
    Article Snippet: Similarly, anti-Gal-9C antibodies suitable for detecting Gal-9C protein are well-known in the art and include, for example, LS-C294015, LS-C301358, LS-C294014, and LS-C304031 (Lifespan Biosciences), sc-292682 (Santa Cruz Biotechnology), PAV236Hu02, PAV236Hu01, and PAV236Hu71 (Cloud-Clone Corporation), orb1189221 and orb184907 (Biorbyt), ARP70764_P050 (Aviva Systems Biology), 140398 and 140399 (United States Biological), and ab178351 (Abcam).

    Western Blot:

    Article Title: Treg-mediated Suppression of Inflammation Induced by DR3 Signaling is Dependent on Galectin-9
    Article Snippet: Lysates were incubated with TL1A.Ig fusion protein bound to protein G Dynabeads (Invitrogen) to precipitate DR3 as performed previously ( 7 ). .. After SDS-PAGE, the proteins were visualized by western blotting with anti-mouse DR3 (R&D Systems) or anti-mouse Galectin-9 (BioLegend) followed by anti-Rat IgG light chain-specific-HRP (Jackson ImmunoResearch). ..

    FACS:

    Article Title: Galectin-9 binds IgM-BCR to regulate B cell signaling
    Article Snippet: C57BL/6, Gal9-KO, or rGal9-treated B cells were first blocked with purified rat anti-mouse CD16/32 (Fc-block, 1:250 v/v, clone FCR-4G8, Invitrogen) in fluorescence-activated cell sorting (FACS) buffer (1% bovine serum albumin (BSA), 4 mM EDTA, and 0.01% NaN 3 in PBS) for 30 min at 4 °C. .. Cells were washed three times with FACS buffer and then immunostained with phycoerythrin-conjugated rat anti-mouse galectin-9 antibody (clone 108A2, Biolegend) at 1:200 v/v, or stained with goat anti-galectin-9 antibody (R&D Systems, Cat. No. AF3535) at 1:100 v/v in FACS buffer for 30 min at 4 °C, followed by Cy3-conjugated AffiniPure bovine anti-goat antibody (Jackson ImmunoResearch Laboratories, Cat. No. 805-165-180) at 1:1000 v/v in FACS buffer for 30 min at 4 °C. .. Cells were then stained with the following antibodies: APC-conjugated anti-CD19 (clone ID3, Biolegend) at 1:200 v/v; Alexa Fluor 488 or Alexa Fluor 644 AffiniPure Fab fragment rat anti-mouse IgM, μ-chain-specific (Jackson ImmunoResearch Laboratories, Cat. No 115-547-020 or Cat. No 115-607-020) at 1:200 v/v in FACS buffer for 30 min at 4 °C.

    Staining:

    Article Title: Galectin-9 binds IgM-BCR to regulate B cell signaling
    Article Snippet: C57BL/6, Gal9-KO, or rGal9-treated B cells were first blocked with purified rat anti-mouse CD16/32 (Fc-block, 1:250 v/v, clone FCR-4G8, Invitrogen) in fluorescence-activated cell sorting (FACS) buffer (1% bovine serum albumin (BSA), 4 mM EDTA, and 0.01% NaN 3 in PBS) for 30 min at 4 °C. .. Cells were washed three times with FACS buffer and then immunostained with phycoerythrin-conjugated rat anti-mouse galectin-9 antibody (clone 108A2, Biolegend) at 1:200 v/v, or stained with goat anti-galectin-9 antibody (R&D Systems, Cat. No. AF3535) at 1:100 v/v in FACS buffer for 30 min at 4 °C, followed by Cy3-conjugated AffiniPure bovine anti-goat antibody (Jackson ImmunoResearch Laboratories, Cat. No. 805-165-180) at 1:1000 v/v in FACS buffer for 30 min at 4 °C. .. Cells were then stained with the following antibodies: APC-conjugated anti-CD19 (clone ID3, Biolegend) at 1:200 v/v; Alexa Fluor 488 or Alexa Fluor 644 AffiniPure Fab fragment rat anti-mouse IgM, μ-chain-specific (Jackson ImmunoResearch Laboratories, Cat. No 115-547-020 or Cat. No 115-607-020) at 1:200 v/v in FACS buffer for 30 min at 4 °C.

    Incubation:

    Article Title: Galectin-9 in allergic airway inflammation and hyper-responsiveness in mice.
    Article Snippet: Background: Galectin-9 (Gal-9) is a member of the growing family of -galactoside-binding lectins.. Gal-9 is an eosinophil chemoattractant and inducer of Th1 cell apoptosis.. These effects suggest its potential role in the pathogenesis of asthma.



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