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temperature gradient functionality  (Bio-Rad)


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    Structured Review

    Bio-Rad temperature gradient functionality
    Temperature Gradient Functionality, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/functional+gradient/C1000+Touch+Firmware+Update/pmc12222638-135-3-5
    Average 99 stars, based on 492 article reviews
    temperature gradient functionality - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutations
    Article Snippet: Total RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Qiagen, 74106) with optional QIAshredder (Qiagen, 79656). .. 1 μg of RNA was reverse transcribed using BioRad iScript reverse transcriptase (Bio-Rad, 1708841) in a Bio-Rad C1000 Touch Thermal Cycler. .. 2 μL of the cDNA served as the template for qPCR using iQ SYBR Green Supermix (Bio-Rad, 1708882) and performed with a BioRad CFX96 Real-Time System. qPCR primers are shown in .

    Incubation:

    Article Title: Methods and compositions for treatment of pancreatic cancer
    Article Snippet: .. After GEM generation, the samples were subjected to an incubation at 53° C. for 45 min in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad, Hercules) to generate poly A cDNA barcoded at the 5′ end by the addition of a template switch oligo (TSO) linked to a cell barcode and Unique Molecular Identifiers (UMIs). .. GEMs were broken, and the single-strand cDNA was cleaned up with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific, Waltham, MA).

    Real-time Polymerase Chain Reaction:

    Article Title: Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice.
    Article Snippet: Quantitative reverse-transcription PCR (qRT-PCR) of miRNAs AR TI CL E IN P RE SS EV-derived RNA (30 ng) was used to synthesize cDNA using miRCURY LNA RT kit (Qiagen). .. The cDNAs were then diluted at 1:30 and qPCR was performed using 2x miRCURY SYBR Green master mix (Qiagen) and miRCURY LNA miRNA PCR Assays (#339306, YP00204772 for miR-23a-3p and #339306, YP00204063 for miR-103-3p, Qiagen) with 95 °C for 2 min, 50 cycles of 95 °C for 10 s, and 56 °C for 60 s, on a C1000 Touch Thermal Cycler with CFX96 Real Time PCR Detection System (BioRad). ..

    Article Title: Identification and Validation of the Breast Cancer Diagnostic Biomarker SVEP1 and Its Relationship With CD8 + T Cell Activation Using Bioinformatics and Machine Learning.
    Article Snippet: Breast cancer (BC) is a malignant disease with high mortality.. Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1 (SVEP1) has been reported to exert tumor-suppressive effects in various cancers when overexpressed; nevertheless, its role in BC development remains unknown.. The Cancer Genome Atlas (TCGA) and ImmPort were mined for BC differentially expressed genes; machine learning plus single gene set enrichment analysis (GSEA) nominated SVEP1 and its pathways.

    SYBR Green Assay:

    Article Title: Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice.
    Article Snippet: Quantitative reverse-transcription PCR (qRT-PCR) of miRNAs AR TI CL E IN P RE SS EV-derived RNA (30 ng) was used to synthesize cDNA using miRCURY LNA RT kit (Qiagen). .. The cDNAs were then diluted at 1:30 and qPCR was performed using 2x miRCURY SYBR Green master mix (Qiagen) and miRCURY LNA miRNA PCR Assays (#339306, YP00204772 for miR-23a-3p and #339306, YP00204063 for miR-103-3p, Qiagen) with 95 °C for 2 min, 50 cycles of 95 °C for 10 s, and 56 °C for 60 s, on a C1000 Touch Thermal Cycler with CFX96 Real Time PCR Detection System (BioRad). ..

    Polymerase Chain Reaction:

    Article Title: Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice.
    Article Snippet: Quantitative reverse-transcription PCR (qRT-PCR) of miRNAs AR TI CL E IN P RE SS EV-derived RNA (30 ng) was used to synthesize cDNA using miRCURY LNA RT kit (Qiagen). .. The cDNAs were then diluted at 1:30 and qPCR was performed using 2x miRCURY SYBR Green master mix (Qiagen) and miRCURY LNA miRNA PCR Assays (#339306, YP00204772 for miR-23a-3p and #339306, YP00204063 for miR-103-3p, Qiagen) with 95 °C for 2 min, 50 cycles of 95 °C for 10 s, and 56 °C for 60 s, on a C1000 Touch Thermal Cycler with CFX96 Real Time PCR Detection System (BioRad). ..

    Article Title: PrP C Glycoprotein Modulates Atmospherically Relevant Artificial Particulate Matter-Induced Development of Lung Cancer in Mice.
    Article Snippet: .. The PCR conditions were as follows: denaturation at 95 °C for 15 min; 34 cycles of 95 °C for 20 s, 56 °C for 40 s, and 72 °C for 1 min for annealing and extension; and final extension at 72 °C for 5 min. PCR was performed using a C1000 Touch Thermal Cycler instrument (Bio-Rad, Hercules, CA). ..

    Article Title: Identification and Validation of the Breast Cancer Diagnostic Biomarker SVEP1 and Its Relationship With CD8 + T Cell Activation Using Bioinformatics and Machine Learning.
    Article Snippet: Breast cancer (BC) is a malignant disease with high mortality.. Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1 (SVEP1) has been reported to exert tumor-suppressive effects in various cancers when overexpressed; nevertheless, its role in BC development remains unknown.. The Cancer Genome Atlas (TCGA) and ImmPort were mined for BC differentially expressed genes; machine learning plus single gene set enrichment analysis (GSEA) nominated SVEP1 and its pathways.

    Article Title: Protecting cells at the genetic level and simulating unauthorized access via a biohackathon
    Article Snippet: .. General cloning was performed by inverse polymerase chain reaction (PCR) on a C1000 Touch Thermal Cycler (Bio-Rad) followed by Kinase, Ligase and DpnI (KLD) reaction. .. Q5 polymerase (NEB) was used for PCR, KLD Enzyme Mix (NEB) and KLD Reaction Buffer (NEB) were used for KLD, NEBuilder HiFi DNA Assembly Master Mix (NEB) was used for Gibson assembly, and T4 DNA ligase (NEB), and Bsa I–HFv2 restriction enzyme (NEB) were used for Golden Gate assembly.

    Amplification:

    Article Title: Complex temporal dynamics of phage-bacteria populations in an animal-associated marine system.
    Article Snippet: The ddPCR reactions were AR TI CL E IN P RE SS incorporated into droplets using the QX100 Droplet Generator (Bio-Rad). .. Nucleic acids were amplified with the following cycling conditions: 5 min at 95 °C, 40 cycles of 30 s at 95 °C and 60 °C for 60s using a Bio-Rad’s C1000 Touch Thermal Cycler. ..

    Article Title: Nicotine Suppresses Human Memory Th Cell Subsets With Preferential Effects on Central Memory Th Cells in an α7 Nicotinic Acetylcholine Receptor-Dependent Manner.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reativ ATA3 (Hs00231122_m1), BCL6 (Hs00153368_m1), and CHRNA7 Hs01063372_m1), with PPIA (Hs99999904_m1) as the houseeeping control. .. Amplification was carried out on a CFX96 eal-Time System C1000 Thermal Cycler (Bio-Rad Laboratories) ith the following cycling conditions: 50◦C for 2 min, 95◦C for 0 s, followed by 50 cycles of 95◦C for 3 s and 60◦C for 30 s. ene expression levels were normalized to the reference gene PIA , and the relative expression levels of TBX21 , RORC , GATA3 , nd BCL6 were calculated using the 2− ΔΔC t method. ..

    Expressing:

    Article Title: Nicotine Suppresses Human Memory Th Cell Subsets With Preferential Effects on Central Memory Th Cells in an α7 Nicotinic Acetylcholine Receptor-Dependent Manner.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reativ ATA3 (Hs00231122_m1), BCL6 (Hs00153368_m1), and CHRNA7 Hs01063372_m1), with PPIA (Hs99999904_m1) as the houseeeping control. .. Amplification was carried out on a CFX96 eal-Time System C1000 Thermal Cycler (Bio-Rad Laboratories) ith the following cycling conditions: 50◦C for 2 min, 95◦C for 0 s, followed by 50 cycles of 95◦C for 3 s and 60◦C for 30 s. ene expression levels were normalized to the reference gene PIA , and the relative expression levels of TBX21 , RORC , GATA3 , nd BCL6 were calculated using the 2− ΔΔC t method. ..

    Cloning:

    Article Title: Protecting cells at the genetic level and simulating unauthorized access via a biohackathon
    Article Snippet: .. General cloning was performed by inverse polymerase chain reaction (PCR) on a C1000 Touch Thermal Cycler (Bio-Rad) followed by Kinase, Ligase and DpnI (KLD) reaction. .. Q5 polymerase (NEB) was used for PCR, KLD Enzyme Mix (NEB) and KLD Reaction Buffer (NEB) were used for KLD, NEBuilder HiFi DNA Assembly Master Mix (NEB) was used for Gibson assembly, and T4 DNA ligase (NEB), and Bsa I–HFv2 restriction enzyme (NEB) were used for Golden Gate assembly.

    Inverse PCR:

    Article Title: Protecting cells at the genetic level and simulating unauthorized access via a biohackathon
    Article Snippet: .. General cloning was performed by inverse polymerase chain reaction (PCR) on a C1000 Touch Thermal Cycler (Bio-Rad) followed by Kinase, Ligase and DpnI (KLD) reaction. .. Q5 polymerase (NEB) was used for PCR, KLD Enzyme Mix (NEB) and KLD Reaction Buffer (NEB) were used for KLD, NEBuilder HiFi DNA Assembly Master Mix (NEB) was used for Gibson assembly, and T4 DNA ligase (NEB), and Bsa I–HFv2 restriction enzyme (NEB) were used for Golden Gate assembly.



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