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phase e coli dh5α  (ATCC)


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    Structured Review

    ATCC phase e coli dh5α
    Bacterial strains and plasmids
    Phase E Coli Dh5α, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/function+from+the+phased+array+system+toolbox/Escherichia+coli+DH5alpha%2C+pIE/pmc06715845-210-11-18
    Average 93 stars, based on 62 article reviews
    phase e coli dh5α - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "New Shuttle Vectors for Real-Time Gene Expression Analysis in Multidrug-Resistant Acinetobacter Species: In Vitro and In Vivo Responses to Environmental Stressors"

    Article Title: New Shuttle Vectors for Real-Time Gene Expression Analysis in Multidrug-Resistant Acinetobacter Species: In Vitro and In Vivo Responses to Environmental Stressors

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01334-19

    Bacterial strains and plasmids
    Figure Legend Snippet: Bacterial strains and plasmids

    Techniques Used: Plasmid Preparation, Cloning, Expressing, Low Copy Number, Clone Assay, Binding Assay

    Transformation efficiency and stability of pLPV plasmids and antibiotic concentrations required for their selection
    Figure Legend Snippet: Transformation efficiency and stability of pLPV plasmids and antibiotic concentrations required for their selection

    Techniques Used: Transformation Assay, Plasmid Preparation

    Physical and functional map of pLPV1Z (A), pLPV2Z (B), and pLPV3Z (C) promoter-probe vectors. The nucleotide sequence upstream of the reporter gene is shown for each vector. In all vectors, the ribosome binding site (RBS) is provided by the canonical sequence 5′-AAAGAGGAGAAA-3′, preceded by stop codons in the three reading frames (red). Unique cutter restriction enzymes are indicated in bold. Nomenclature: ble, Zeo resistance gene; aacC1, Gm resistance gene; ori ColE1-like, ColE1-derived origin of replication for E. coli; oriAb, origin of replication for Acinetobacter spp. All genes are reported in scale over the total length of the vector. Images were obtained by Snapgene software (GSL Biotech).
    Figure Legend Snippet: Physical and functional map of pLPV1Z (A), pLPV2Z (B), and pLPV3Z (C) promoter-probe vectors. The nucleotide sequence upstream of the reporter gene is shown for each vector. In all vectors, the ribosome binding site (RBS) is provided by the canonical sequence 5′-AAAGAGGAGAAA-3′, preceded by stop codons in the three reading frames (red). Unique cutter restriction enzymes are indicated in bold. Nomenclature: ble, Zeo resistance gene; aacC1, Gm resistance gene; ori ColE1-like, ColE1-derived origin of replication for E. coli; oriAb, origin of replication for Acinetobacter spp. All genes are reported in scale over the total length of the vector. Images were obtained by Snapgene software (GSL Biotech).

    Techniques Used: Functional Assay, Sequencing, Plasmid Preparation, Binding Assay, Derivative Assay, Software

    Related Articles

    Bacteria:

    Article Title: Magnetic nanoparticles tethered with Zn–DPA for the removal of bacteria from red blood cell suspension
    Article Snippet: .. Five strains of bacteria were studied: Staphylococcus aureus ( S. aureus , strain ATCC #12600), Staphylococcus epidermidis ( S. epidermidis , strain RP62A), Pseudomonas aeruginosa ( P. aeruginosa , strain PAO1), Escherichia coli ( E. coli ) DH5α and E. coli ATCC #25922. ..

    other:

    Article Title: Lavandula pedunculata subsp. atlantica : A Multifunctional Essential Oil for Potentially Combating Microbial Infections and Inflammatory Processes.
    Article Snippet: The microbiological effect was assessed using the following bacterial strains: Gramnegative: Escherichia coli DH5α and Shigella sonnei ATCC25931; and Gram-positive: Staphylococcus aureus ATCC6538P, Streptococcus oralis CECT 8313 and Streptococcus mutans ATCC 35668.

    Cloning:

    Article Title:
    Article Snippet: .. Used as a surrogate platform in competition assays and in secretion assays 3 Vibrio natriegens ATCC 14048 Wild type Used as prey in competition assays ATCC collection Vibrio coralliilyticus ATCC BAA-450 Wild type Used for generating deletion strains, as the attacker in competition assays, and in secretion assays ATCC collection Vibrio coralliilyticus ATCC BAA-450 ∆hcp1 ∆vic_rs16330 ATCC BAA-450 derivative containing an inframe deletion of vic_rs16330; used as the attacker in competition assays, and in secretion assays 3 Vibrio campbellii ATCC 25920 Wild type Used for cloning ATCC collection Escherichia coli DH5α (λ pir) K-12 derivative laboratory strain containing λ pir Used for plasmid maintenance and cloning Obtained from Eric V. Stabb Escherichia coli BL21 (DE3) Laboratory strain Used for protein expression and toxicity assays Obtained from Kim Orth Table S2. ..

    Article Title:
    Article Snippet: .. E. coli DH5α Plasmid maintenance E. coli strain used for all cloning in this study Lab stock E. coli HB101 E. coli mating strain Lab stock A. baumannii 17978VU Used for WT in this study Lab stock A. baumannii ∆alkX 17978VU harboring an ahp kanamycin cassette insertion into the alkX locus This study A. baylyi ATCC 33305 Typed ADP1 strain Lab stock E. coli Tuner (DE3) Used for protein expression in this study Lab stock ..

    Article Title: PGN_0298 in the Assembly and Insertion Machinery (Aim) Operon Is Essential for the Viability of Porphyromonas gingivalis.
    Article Snippet: .. Strain Escherichia coli DH5α General purpose host strain for cloning Nippongene, Toyama, Japan P. gingivalis ATCC 33277 Wild type American Type Culture Collection 33277::pPGN_0298 ATCC 33277 and pPGN_0298 This study ΔPGN_0298::pPGN_0298 ATCC 33277 ΔPGN_0298 and pPGN_0298 This study Plasmid pBluescript II SK(+) Apr, cloning vector Stratagene pKD355 AprEmr, contains the ermF ermAM DNA cassette in pUC19 Ueshima et al. (14) pTIO‐1 AprEmr, contains the ermF DNA in pBSSK Tagawa et al. (15) pBSPGN_0298 Apr, pBSSK containing PGN_0298::ermF This study pPGN_0298 AprtetQ contains the rgpB promoter and PGN_0298 in PBSSK This study 2 Microbiology and Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/1348-0421.70021 by N at Prov Indonesia, W iley O nline L ibrary on [07/11/2025]. ..

    Plasmid Preparation:

    Article Title:
    Article Snippet: .. Used as a surrogate platform in competition assays and in secretion assays 3 Vibrio natriegens ATCC 14048 Wild type Used as prey in competition assays ATCC collection Vibrio coralliilyticus ATCC BAA-450 Wild type Used for generating deletion strains, as the attacker in competition assays, and in secretion assays ATCC collection Vibrio coralliilyticus ATCC BAA-450 ∆hcp1 ∆vic_rs16330 ATCC BAA-450 derivative containing an inframe deletion of vic_rs16330; used as the attacker in competition assays, and in secretion assays 3 Vibrio campbellii ATCC 25920 Wild type Used for cloning ATCC collection Escherichia coli DH5α (λ pir) K-12 derivative laboratory strain containing λ pir Used for plasmid maintenance and cloning Obtained from Eric V. Stabb Escherichia coli BL21 (DE3) Laboratory strain Used for protein expression and toxicity assays Obtained from Kim Orth Table S2. ..

    Article Title:
    Article Snippet: .. E. coli DH5α Plasmid maintenance E. coli strain used for all cloning in this study Lab stock E. coli HB101 E. coli mating strain Lab stock A. baumannii 17978VU Used for WT in this study Lab stock A. baumannii ∆alkX 17978VU harboring an ahp kanamycin cassette insertion into the alkX locus This study A. baylyi ATCC 33305 Typed ADP1 strain Lab stock E. coli Tuner (DE3) Used for protein expression in this study Lab stock ..

    Article Title: PGN_0298 in the Assembly and Insertion Machinery (Aim) Operon Is Essential for the Viability of Porphyromonas gingivalis.
    Article Snippet: .. Strain Escherichia coli DH5α General purpose host strain for cloning Nippongene, Toyama, Japan P. gingivalis ATCC 33277 Wild type American Type Culture Collection 33277::pPGN_0298 ATCC 33277 and pPGN_0298 This study ΔPGN_0298::pPGN_0298 ATCC 33277 ΔPGN_0298 and pPGN_0298 This study Plasmid pBluescript II SK(+) Apr, cloning vector Stratagene pKD355 AprEmr, contains the ermF ermAM DNA cassette in pUC19 Ueshima et al. (14) pTIO‐1 AprEmr, contains the ermF DNA in pBSSK Tagawa et al. (15) pBSPGN_0298 Apr, pBSSK containing PGN_0298::ermF This study pPGN_0298 AprtetQ contains the rgpB promoter and PGN_0298 in PBSSK This study 2 Microbiology and Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/1348-0421.70021 by N at Prov Indonesia, W iley O nline L ibrary on [07/11/2025]. ..

    Expressing:

    Article Title:
    Article Snippet: .. Used as a surrogate platform in competition assays and in secretion assays 3 Vibrio natriegens ATCC 14048 Wild type Used as prey in competition assays ATCC collection Vibrio coralliilyticus ATCC BAA-450 Wild type Used for generating deletion strains, as the attacker in competition assays, and in secretion assays ATCC collection Vibrio coralliilyticus ATCC BAA-450 ∆hcp1 ∆vic_rs16330 ATCC BAA-450 derivative containing an inframe deletion of vic_rs16330; used as the attacker in competition assays, and in secretion assays 3 Vibrio campbellii ATCC 25920 Wild type Used for cloning ATCC collection Escherichia coli DH5α (λ pir) K-12 derivative laboratory strain containing λ pir Used for plasmid maintenance and cloning Obtained from Eric V. Stabb Escherichia coli BL21 (DE3) Laboratory strain Used for protein expression and toxicity assays Obtained from Kim Orth Table S2. ..

    Article Title:
    Article Snippet: .. E. coli DH5α Plasmid maintenance E. coli strain used for all cloning in this study Lab stock E. coli HB101 E. coli mating strain Lab stock A. baumannii 17978VU Used for WT in this study Lab stock A. baumannii ∆alkX 17978VU harboring an ahp kanamycin cassette insertion into the alkX locus This study A. baylyi ATCC 33305 Typed ADP1 strain Lab stock E. coli Tuner (DE3) Used for protein expression in this study Lab stock ..

    Membrane:

    Article Title: Label-free phenotypic antimicrobial susceptibility testing on microfluidic platforms: a review of advances and translation.
    Article Snippet: and economic burden is severe, as resistant infections often require expensive or toxic therapies, lead to prolonged hospital stays, and result in higher mortality rates [5–7].. A cornerstone strategy for mitigating AMR is the development of rapid, reliable, and accessible diagnostic methods that guide appropriate antibiotic use while reducing over-prescription.. AMR arises when microorganisms develop mechanisms to neutralize or evade antimicrobial agents [8, 9].

    Filtration:

    Article Title: Label-free phenotypic antimicrobial susceptibility testing on microfluidic platforms: a review of advances and translation.
    Article Snippet: and economic burden is severe, as resistant infections often require expensive or toxic therapies, lead to prolonged hospital stays, and result in higher mortality rates [5–7].. A cornerstone strategy for mitigating AMR is the development of rapid, reliable, and accessible diagnostic methods that guide appropriate antibiotic use while reducing over-prescription.. AMR arises when microorganisms develop mechanisms to neutralize or evade antimicrobial agents [8, 9].

    Inhibition:

    Article Title: Recent advance on the antibacterial activity of polysaccharide-based conjugates.
    Article Snippet: Polysaccharide has attracted global attention because of its potential application as an alternative to antibiotics to alleviate antibiotic resistance and drug residue caused by antibiotic abuse, but its relatively low antibacterial activity and narrow antibacterial spectrum cannot meet the needs of daily life and clinical treatment.. Apart from chemical, physical, and biological modifications, conjugation with other bioactive compounds via covalent or non-covalent interactions can not only improve the antibacterial activity and reduce the bacterial resistance of polysaccharide-based conjugates, but also expand the antibacterial spectrum and reduce the cytotoxicity of polysaccharide-based conjugates.. Then, a systematic and up-to-date overview of the conjugation strategies aimed at enhancing the antibacterial activity of polysaccharides will guide the future development of polysaccharide conjugates with enhanced antibacterial properties.

    Negative Control:

    Article Title: Luminescence-Based Strategies for Detecting β-Lactamase Activity: A Review of the Last Decade.
    Article Snippet: .. An antibioticsusceptible E. coli DH5α strain (ATCC 53868) was used as the negative control. ..



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