frap data analysis (Nikon)
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Frap Data Analysis, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 40098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 40098 article reviews
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Confocal:Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model. Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes. Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes. Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat. Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Microscopy:Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model. Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes. Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes. Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring. Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20. Article Title: Discovery of a Virulent Population of Meloidogyne graminicola Overcoming the Rice Resistance Gene MG1 and Population Genetic Analysis Based on mtCOI Sequences. Article Snippet: .. Measurements and photographs were https://doi.org/10.3390/plants15193031 taken with a Nikon ECLIPSE Ni microscope equipped with a Nikon Digital Sight Camera and Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat. Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Software:Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model. Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes. Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes. Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring. Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20. Article Title: Discovery of a Virulent Population of Meloidogyne graminicola Overcoming the Rice Resistance Gene MG1 and Population Genetic Analysis Based on mtCOI Sequences. Article Snippet: .. Measurements and photographs were https://doi.org/10.3390/plants15193031 taken with a Nikon ECLIPSE Ni microscope equipped with a Nikon Digital Sight Camera and Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon Fluorescence:Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring. Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20. Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat. Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Automated:Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the Imaging:Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes. Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes. Inverted Microscopy:Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes. Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes. Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon Analysis:Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes. Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes. Light Microscopy:Article Title: Leptin promotes obesity‑related osteoarthritis progression via CD44/JAK2/STAT3 axis‑mediated chondrocyte apoptosis. Article Snippet: .. Following dehydration in increasing ethanol concentrations (70‐100%) and xylene clearing, the slides were mounted with neutral balsam and images were captured under a Nikon Eclipse 80i light microscope (Nikon Corporation,) using Polymerase Chain Reaction:Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat. Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Amplification:Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat. Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Expressing:Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat. Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a |
Table S3 ). (G) Scheme showing 97 Hz and 2 Hz SPT workflow. Three-state model fits to 97 Hz SPT data using Spot-On was used to derive fractions and diffusion coefficients of fast diffusing ( F fast , D fast ), slow diffusing ( F slow , D slow ), and bound PARP ( F bound , D bound ) molecules. Further, 2 Hz SPT data were fit using a two-phase exponential model to derive fractions and duration of transient (Fraction transient, τ transient ) and stable (Fraction stable, τ stable ) PARP binding events. (H and I) Pie chart illustrations summarizing the derivation of overall fractions of Halo-PARP1 (in H) and Halo-PARP2 (in I) engaging in transient and stable binding, slow diffusion, and fast diffusion from 97 to 2 Hz SPT experiments. The bound, slow, and fast-diffusing fractions (in i) were determined using Spot-On’s three-state model fitting to 97 Hz SPT data. The bound fraction (in ii) in Halo-PARP1 and Halo-PARP2 cells was analyzed by 2 Hz SPT and fit to a two-phase exponential model. Data acquired for H2B-Halo were used for photobleaching correction, and a correction factor (see ) was applied to obtain the true fraction of transiently and stably binding Halo-PARP molecules (in iii). These data were compiled together to obtain the overall fractions of endogenous Halo-PARP1 and Halo-PARP2 molecules (in iv). (J) Normalized and photobleaching corrected recovery curves from 