assay buffer (Jackson Immuno)

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Jackson Immuno assay buffer
Assay Buffer, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fragmentation+buffer/Allophycocyanin+(APC)+AffiniPure+F(ab')%E2%82%82+Fragment+Donkey+Anti-Mouse+IgG/pm41686821-99-7-23
Average 93 stars, based on 30 article reviews

assay buffer - by Bioz Stars, 2026-10

93/100 stars

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Incubation:

Article Title: Parenterally administered pegbovigrastim alters leukocyte counts, granulocyte functions, and uterine cell population in healthy postpartum dairy cows.
Article Snippet: To label granulocytes, 50 μL of anti-bovine granulocyte monoclonal antibody (clone CH138A, Cat #WS0609B-100, Kingfisher Biotech, Saint Paul, MN, USA; 1:50 dilution) was added to all tubes except the isotype control and incubated on ice for 15 minutes. .. After two washes with 400 μL of assay buffer, 100 μL of secondary antibody (Allophycocyanin AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG, Cat #715-136-151, Jackson ImmunoResearch, PA, USA; 1:100 dilution) was added, followed by a second 15-minute ice incubation. ..

Article Title: Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response.
Article Snippet: .. Incubation with mouse antiALP monoclonal antibodies (Bio-Techne (R&D), MAB1448) was performed for 1 hour at room temperature and followed by incubation with anti-mouse Alexa Fluor 647-conjugated antibodies (Jackson Immuno Research, 715-136-150). .. To detect nuclei of the cells on the slides, the mounting medium 4’,6– Diamino-2-Phenylinsole, Dihydrochloride (DAPI) was used (Vector Laboratories, H-1500).

Article Title: Human CD47-targeting single-domain antibody and use thereof
Article Snippet: After rinsing twice with PBS+1% FBS, 1:500 diluted anti-c-myc antibody (Roche, Cat. No. 11667149001) was added to the VHH sample wells and incubated at 4° C. for 30 mins. .. After the cells were rinsed twice with FACS buffer, 1:300 dilution of the detection antibody Allophycocyanin donkey anti-mouse IgG (Jackson Immunoresearch, Cat. No. 715-136-150) was added to the cells, and incubated at 4° C. for 30 mins in the dark. ..

Article Title: Blocking Cx43 alleviates P2X4 receptor-mediated neuropathic pain in CCI rats
Article Snippet: Afterwards, the sections were incubated overnight at 4°C with the following primary antibody mixture: P2X4(1:100, rabbit, Alomone), Cx43(1:100, goat, Sigma), and GFAP (1:100, mouse, Biolegend). .. The next day, the sections were washed 3 times with 0.01, sections were incubated for 1 hour at room temperature with the following mixture of secondary antibodies: TRITC-conjugated donkey anti-rabbit (1:200, donkey, Jackson ImmunoResearch), FITC-conjugated donkey anti-goat (1:200, donkey, Jackson ImmunoResearch), and allophycocyanin-conjugated donkey anti-mouse (1:200, donkey, Jackson ImmunoResearch). .. The images were nally captured using an Olympus laser scanning confocal microscope (FV3000).

Article Title: Parenterally administered pegbovigrastim alters leukocyte counts, granulocyte functions, and uterine cell population in healthy postpartum dairy cows
Article Snippet: To label granulocytes, 50 μL of anti-bovine granulocyte monoclonal antibody (clone CH138A, Cat #WS0609B-100, Kingfisher Biotech, Saint Paul, MN, USA; 1:50 dilution) was added to all tubes except the isotype control and incubated on ice for 15 minutes. .. After two washes with 400 μL of assay buffer, 100 μL of secondary antibody (Allophycocyanin AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG, Cat #715-136-151, Jackson ImmunoResearch, PA, USA; 1:100 dilution) was added, followed by a second 15-minute ice incubation. ..

Article Title: Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response
Article Snippet: .. Incubation with mouse anti-ALP monoclonal antibodies (Bio-Techne (R&D), MAB1448) was performed for 1 hour at room temperature and followed by incubation with anti-mouse Alexa Fluor 647-conjugated antibodies (Jackson Immuno Research, 715-136-150). .. To detect nuclei of the cells on the slides, the mounting medium 4’,6–Diamino-2-Phenylinsole, Dihydrochloride (DAPI) was used (Vector Laboratories, H-1500).

Bioprocessing:

FACS:

Staining:

Article Title: Immune-induced TCR-like antibodies regulate specific T cell response
Article Snippet: .. The cells were stained with immunized mouse serum at a dilution of 1:100-1000 and then reacted with allophycocyanin (APC)-conjugated anti-mouse IgG (715-136-151, Jackson ImmunoResearch Laboratories) or Alexa Fluor 647-conjugated anti-mouse IgG1(A21240, Invitrogen) for LK35.2 cell staining as a secondary antibody. .. The Aw3.18 antibody was purified from the supernatant of a hybridoma culture (CRL-2826, ATCC).

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