buffer (Jackson Immuno)
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Jackson Immuno
buffer
Buffer, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fragmentation+buffer/Cy+3+AffiniPure+F(ab')%E2%82%82+Fragment+Donkey+Anti-Rat+IgG/pm41299058-243-10-13
Average 94 stars, based on 107 article reviews
Buffer, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fragmentation+buffer/Cy+3+AffiniPure+F(ab')%E2%82%82+Fragment+Donkey+Anti-Rat+IgG/pm41299058-243-10-13
Average 94 stars, based on 107 article reviews
buffer - by Bioz Stars,
2026-09
94/100 stars
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other:Article Title: Molecular determinants of cardiac lymphatic dysfunction in a chronic pressure-overload model Article Snippet: Staining:Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers Article Snippet: .. For staining, DNA fibres were denatured with 2.5 M HCl for 1 hour, washed with PBS and blocked with 0.2% Tween 20 (v/v) in 1% BSA/PBS for 40 min. Immunolabelling of CldU and IdU tracks was performed with anti-BrdU antibodies as described above (for 2.5 h in the dark, at room temperature), followed by 1 h incubation with secondary antibodies in the dark at room temperature, using anti-mouse Alexa Fluor 488 (A11001, Invitrogen, 1:300) and Incubation:Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers Article Snippet: .. For staining, DNA fibres were denatured with 2.5 M HCl for 1 hour, washed with PBS and blocked with 0.2% Tween 20 (v/v) in 1% BSA/PBS for 40 min. Immunolabelling of CldU and IdU tracks was performed with anti-BrdU antibodies as described above (for 2.5 h in the dark, at room temperature), followed by 1 h incubation with secondary antibodies in the dark at room temperature, using anti-mouse Alexa Fluor 488 (A11001, Invitrogen, 1:300) and Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers. Article Snippet: .. Immunolabellingof CldU and IdU tracks was performed with anti-BrdU antibodies as described above (for 2.5 h in the dark, at room temperature), followed by 1 h incubation with secondary antibodies in the dark at room temperature, using anti-mouse Alexa Fluor 488 (A11001, Invitrogen, 1:300) and Article Title: The mineralocorticoid receptor antagonist finerenone enhances cardiovascular recovery upon food intake normalization in obese mice. Article Snippet: .. To reveal endothelial cells, sections were incubated for 1 h with a primary antibody rat anti-mouse CD31 (1:100) (553,371, BD, USA) in 1% BSA-PBS buffer with 0.3% saponin, washed 3 × 5 min with PBS and incubated for 30 min in the dark with the secondary antibody (1:400) ( Article Title: Combination therapy overcomes secondary PARPi resistance in ATM-deficient prostate cancer Article Snippet: Slides were stained with primary antibodies diluted in blocking buffer (Rat α CldU (Abcam cat# ab6326; 1:200), Mouse α IdU (Becton–Dickinson; cat# 34758 1:100) for 2 h at RT in the dark, washed three times with PBST and then incubated with 2% (w/v) BSA in PBST (0.1%) for 10 min. .. Slides were next incubated with secondary antibodies diluted in blocking Article Title: Combination therapy overcomes secondary PARPi resistance in ATM-deficient prostate cancer. Article Snippet: Slides were stained with primary antibodies diluted in blocking buffer (Rat α CldU (Abcam cat# ab6326; 1:200), Mouse α IdU (Becton Dickinson; cat# 34758 1:100) for 2 hours at RT in 510 AR TI CL E IN P RE SS 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 dark, washed three times with PBST and then incubated with 2% (w/v) BSA in PBST (0.1%) for 10 min. .. Slides were next incubated with secondary antibodies diluted in blocking Article Title: CSF1R ligands promote microglial proliferation but are not the sole regulators of developmental microglial proliferation Article Snippet: We diluted the primary antibodies – rabbit anti-IBA1 (1:1000; Wako, 019-19741), goat anti-IBA1 (1:400; Novus Biologicals NB100-1028), rabbit anti-Tmem119 (1:400; Abcam, ab209064) and rat anti-KI67 (1:200; Invitrogen, 14-5698-82) in a buffer of 0.1% gelatine from cold water fish skin and 0.1% Triton X-100 in 0.01 M PBS and incubated slides with primary antibodies at 4°C overnight. .. We washed slides three to six times with PBS and incubated slides with secondary antibodies anti-rabbit Alexa Fluor 488 (1:400; Jackson ImmunoResearch, 711-546-152), anti-goat Alexa Fluor 488 (1:400; Jackson ImmunoResearch, 705-546-147), Article Title: The mineralocorticoid receptor antagonist finerenone enhances cardiovascular recovery upon food intake normalization in obese mice Article Snippet: .. To reveal endothelial cells, sections were incubated for 1 h with a primary antibody rat anti-mouse CD31 (1:100) (553,371, BD, USA) in 1% BSA-PBS buffer with 0.3% saponin, washed 3 × 5 min with PBS and incubated for 30 min in the dark with the secondary antibody (1:400) ( Blocking Assay:Article Title: Combination therapy overcomes secondary PARPi resistance in ATM-deficient prostate cancer Article Snippet: Slides were stained with primary antibodies diluted in blocking buffer (Rat α CldU (Abcam cat# ab6326; 1:200), Mouse α IdU (Becton–Dickinson; cat# 34758 1:100) for 2 h at RT in the dark, washed three times with PBST and then incubated with 2% (w/v) BSA in PBST (0.1%) for 10 min. .. Slides were next incubated with secondary antibodies diluted in blocking Article Title: Combination therapy overcomes secondary PARPi resistance in ATM-deficient prostate cancer. Article Snippet: Slides were stained with primary antibodies diluted in blocking buffer (Rat α CldU (Abcam cat# ab6326; 1:200), Mouse α IdU (Becton Dickinson; cat# 34758 1:100) for 2 hours at RT in 510 AR TI CL E IN P RE SS 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 dark, washed three times with PBST and then incubated with 2% (w/v) BSA in PBST (0.1%) for 10 min. .. Slides were next incubated with secondary antibodies diluted in blocking |