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log-dose vs response -variable slope (four parameters) function of the graphpad prism computer program  (GraphPad Software Inc)


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    GraphPad Software Inc log-dose vs response -variable slope (four parameters) function of the graphpad prism computer program
    Log Dose Vs Response Variable Slope (Four Parameters) Function Of The Graphpad Prism Computer Program, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/four-parameter+dose-response+function/non+linear+dose+response+curve+fitting+analysis/pm25978762-714-3-13
    Average 90 stars, based on 1 article reviews
    log-dose vs response -variable slope (four parameters) function of the graphpad prism computer program - by Bioz Stars, 2026-09
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    Article Title: Structural and mechanistic profiling of Nurr1 modulation by vidofludimus enables structure-guided ligand design
    Article Snippet: For dose-response curve fitting and calculation of EC 50 values, the equation “[Agonist] versus response–variable slope (four parameters)” was used in GraphPad Prism (version 9.5.1, GraphPad Software, La Jolla, CA).

    Article Title: Acetaminophen inhibits diacylglycerol lipase synthesis of 2-arachidonoyl glycerol: Implications for nociception
    Article Snippet: The data were fitted with a nonlinear regression (Sigmoidal dose response; GraphPad Prism 6, La Jolla, CA), allowing calculation of an ED 50 , the effective dose or duration of depolarization at which a 50% inhibition is achieved.

    Article Title: Synthesis, characterization, and biological activity evaluation of 4,6-dihydropyrano[3,2-c]isochromene-3-carbonitrile as anticancer agents.
    Article Snippet: The IC50 values were calculated using a non-linear curve of dose-response in GraphPad® Prism from the percent of viable cells vs. logarithm of concentrations.

    Article Title: Pharmacological Characterization of Dopamine Receptor DRD1 Variants and Exploration of Their Allosteric Activation.
    Article Snippet: G protein-coupled receptors (GPCRs) are major drug targets, yet genetic variations in these receptors can alter drug responses, leading to significant challenges in healthcare.. Despite the prevalence of GPCR-targeting drugs, the effects of these genetic variations on receptor function remain underexplored.. This study establishes a framework for allosterically rescuing loss-of-function (LoF) variants in GPCRs, using the dopamine receptor D1 (DRD1) as a model. We characterized 49 DRD1 variants from genetic databases and literature, finding that most variants exhibit reduced membrane expression.

    Article Title:
    Article Snippet: Data were processed using the nonlinear regression (curve fit) dose– response function in GraphPad Prism 8 (GraphPad Software).

    Article Title: Supporting Information for Cryo-EM structures of human GPR34 enable the discovery of selective antagonists
    Article Snippet: Data were processed using the nonlinear regression (curve fit) dose-response function in GraphPad Prism 8.0.

    Article Title: Salts and solid forms of (S)-3-(4-((4-(morpholinomethyl)benzyl)oxy)-1-oxoisoindolin-2-yl)piperidine-2,6-dione and compositions comprising and methods of using the same
    Article Snippet: IL-2 and MIP-3a, levels are normalized to the amount produced in the presence of an amount of a compound provided herein, and EC50s calculated using non-linear regression, sigmoidal dose-response, constraining the top to 100% and bottom to 0%, allowing variable slope (GraphPad Prism v3.02).



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    vmab-mIL22 exhibits dual functionality, and the functional integrity of the cytokine payloads is preserved. (A, B) Biacore sensorgrams of the interaction of vmab with mIL17A and BIAcore sensorgrams of the interaction of vmab-mIL22 with mIL17A. vmab-mIL22 retained a high affinity for the cognate antigen, similar to that of the parental antibody Vmab, which was confirmed by surface plasmon resonance analysis. Sensorgrams display the response values at IL17A concentrations of 0.01 to 0.2 μg/mL, with a CM5 chip and capture with 100 RU vmab or vmab-mIL22. The equilibrium dissociation constant (K D ) was calculated using k d /k a for each measurement. k d , dissociation rate constant or off-rate constant; k a , association rate constant or on-rate constant. Binding was biphasic, and the data were fit with heterogeneous ligand models. (C) In vitro inhibition of functional activity by vmab-mIL22. Inhibition of Groα release from HT-29 cells as a measure of IL-17A inhibition. HT29 cells were stimulated with IL-17A (5 nM) in the presence of various concentrations of vmab-mIL22. vmab was used as a positive control. Groα levels in the supernatants were measured by ELISA from R&D systems. (D) Western blot analysis of STAT3 phosphorylation (p-STAT3) induced by 10 nM vmab-hIL22 in HepG2 cells at different times. (E) Western blot analysis of STAT3 phosphorylation (p-STAT3) induced by 10 nM mIL-22Fc or vmab-mIL22 in HepG2 cells at 1.5 h Serum-starved cells (3×10 6 /mL) were stimulated with PBS, 10 nM mIL22-Fc or Vmab-mIL22 for 1.5 h at 37°C. Whole-cell lysates were resolved on denaturing gels, transferred, and immunoblotted with a polyclonal anti-phospho-STAT3 antibody.

    Journal: Frontiers in Immunology

    Article Title: A Novel Bifunctional Fusion Protein, Vunakizumab-IL22, for Protection Against Pulmonary Immune Injury Caused by Influenza Virus

    doi: 10.3389/fimmu.2021.727941

    Figure Lengend Snippet: vmab-mIL22 exhibits dual functionality, and the functional integrity of the cytokine payloads is preserved. (A, B) Biacore sensorgrams of the interaction of vmab with mIL17A and BIAcore sensorgrams of the interaction of vmab-mIL22 with mIL17A. vmab-mIL22 retained a high affinity for the cognate antigen, similar to that of the parental antibody Vmab, which was confirmed by surface plasmon resonance analysis. Sensorgrams display the response values at IL17A concentrations of 0.01 to 0.2 μg/mL, with a CM5 chip and capture with 100 RU vmab or vmab-mIL22. The equilibrium dissociation constant (K D ) was calculated using k d /k a for each measurement. k d , dissociation rate constant or off-rate constant; k a , association rate constant or on-rate constant. Binding was biphasic, and the data were fit with heterogeneous ligand models. (C) In vitro inhibition of functional activity by vmab-mIL22. Inhibition of Groα release from HT-29 cells as a measure of IL-17A inhibition. HT29 cells were stimulated with IL-17A (5 nM) in the presence of various concentrations of vmab-mIL22. vmab was used as a positive control. Groα levels in the supernatants were measured by ELISA from R&D systems. (D) Western blot analysis of STAT3 phosphorylation (p-STAT3) induced by 10 nM vmab-hIL22 in HepG2 cells at different times. (E) Western blot analysis of STAT3 phosphorylation (p-STAT3) induced by 10 nM mIL-22Fc or vmab-mIL22 in HepG2 cells at 1.5 h Serum-starved cells (3×10 6 /mL) were stimulated with PBS, 10 nM mIL22-Fc or Vmab-mIL22 for 1.5 h at 37°C. Whole-cell lysates were resolved on denaturing gels, transferred, and immunoblotted with a polyclonal anti-phospho-STAT3 antibody.

    Article Snippet: The mean values of repeated wells were plotted and the standard deviation was calculated using the four parameter dose response inhibition function in Graphpad prism.

    Techniques: Functional Assay, SPR Assay, Binding Assay, In Vitro, Inhibition, Activity Assay, Positive Control, Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics