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solution of equal parts 4xssc and deionized formamide am9342  (Thermo Fisher)


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    Thermo Fisher solution of equal parts 4xssc and deionized formamide am9342
    Solution Of Equal Parts 4xssc And Deionized Formamide Am9342, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/formamide+solution/formamide+thermo+fisher+am9342/pm40108792-106-16-17
    Average 90 stars, based on 1 article reviews
    solution of equal parts 4xssc and deionized formamide am9342 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Heterozygous GAA knockout is nonconsequential on metabolism and the spatial liver transcriptome in high-fat diet-induced obese and prediabetic mice.
    Article Snippet: Slides were covered with HybriSlip hybridization covers (Grace Bio- Labs, 714,022) and hybridized overnight at 37°C in a hybridization oven (Boekel Scientific RapidFISH Slide Hybridizer, Cat#240200). .. Following hybridization, unbound probes were washed away using a solution of equal parts 4XSSC and deionized formamide (Thermo Fisher, AM9342). .. After washing, immunofluorescence staining was performed using commercially available antibodies against E- Cadherin (BD Biosciences, Cat#610182) and Cytochrome P450 2E1 (Abcam, ab28146).

    Article Title: Highly Multiplexed Spatial Transcriptomics in Bacteria
    Article Snippet: .. 10 50X Expansion for Liquid Culture To hybridize the rRNA probes, permeabilized cells were washed twice in 1 mL RNA FISH wash buffer (30% v/v formamide [Thermo, AM9342] in 2×SSC [Thermo, AM9765]), and then resuspended in 50 μL RNA FISH hybridization buffer (30% v/v formamide, 10% w/v dextran 15 sulfate [Millipore, S4030], and 1 mg/mL yeast tRNA [Thermo, 15401029] in 2×SSC) supplemented with the appropriate rRNA probe at a final concentration of 8 μM. ..

    Article Title: Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis.
    Article Snippet: Cells were post-fixed using 5 mM Bis(NHS)PEG5 (803537; Sigma-Aldrich) in PBS for 30 min at RT to preserve cell fixation during primary FISH library hybridization at 37°C. .. Hybridization of primary FISH probe libraries targeting 1.2 Mb regions (Chr14 50.92–52.10 Mb, Chr5 149.50–150.70 Mb, Chr2 191.11–192.31 Mb) with 12 kb genomic resolution (one trace-spot = tiled set of ∼150 FISH probes with common docking handle) was performed by incubation with hybridization buffer (50% formamide [FA, AM9342; Thermo Fisher Scientific], 10% [wt/vol] dextran sulfate [D8906; SigmaAldrich] in 2xSSC [AM9763; Thermo Fisher Scientific]) containing the FISH probe libraries at a final concentration of 100–200 ng/μl DNA per library for one to two nights at 37°C in a humidified chamber. ..

    other:

    Article Title: Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brain.
    Article Snippet: Article Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brain Graphical abstract Highlights • The velum interpositum (VI) is a site for myeloid cell entry into the murine brain • The VI is a leptomeningeal extra-parenchymal structure • The VI contains meningeal macrophages and peripheral myeloid cells • Distinct myeloid cells utilize the VI during development and demyelinating disease Authors Lindsay A. Hohsfield, Sung Jin Kim, Rocio A. Barahona, ..., Xiangmin Xu, Thomas E. Lane, Kim N. Green Correspondence lhohsfie@uci.edu (L.A.H.), kngreen@uci.edu (K.N.G.). In brief Hohsfield et al. reveal a novel gateway for immune cells to enter the brain: the velum interpositum.. This structure serves as a crucial entry point for myeloid cells under various brain states.

    Article Title: Pre-assembly of biomolecular condensate seeds drives RSV replication
    Article Snippet: RSV Nucleoprotein Antibody (RSV3132 (B023)) (referred to as anti-RSV N) (NOVUS Biologicals, cat. no. NB100-64752) was fluorescently-labelled using N-hydroxysuccinimide (NHS)-esters (Alexa Fluor 555 NHS ester and Alexa Fluor 647 NHS ester) (Thermo Fisher Scientific, AF555: cat. no. A20009, AF647: cat. no. A20106) for use in immunofluorescence.

    Article Title: A lncRNA-mediated metabolic rewiring of cell senescence
    Article Snippet: Formamide , Thermo Fisher , AM9342.

    Article Title:
    Article Snippet: The coverslips were briefly rehydrated with Wash Buffer A (SMF-WA1-60, Biosearch) (Formamide, Thermo Fischer Scientific, AM9342) before FISH.

    Article Title: Single-Cell Phenotypic Heterogeneity Shapes Quorum Signaling Dynamics in Pseudomonas aeruginosa
    Article Snippet: Cells were pelleted and resuspended in 20 μL of probe solution (probes in nuclease-free water), then mixed with 30 μL of pre-warmed primary hybridization buffer composed of 50% formamide (Thermo Fisher, AM9342), 10% dextran sulfate (Sigma-Aldrich, D8906), and 2X saline-sodium citrate (SSC) (Gibco LifeTech, 15557-036).

    Fluorescence In Situ Hybridization:

    Article Title: Highly Multiplexed Spatial Transcriptomics in Bacteria
    Article Snippet: .. 10 50X Expansion for Liquid Culture To hybridize the rRNA probes, permeabilized cells were washed twice in 1 mL RNA FISH wash buffer (30% v/v formamide [Thermo, AM9342] in 2×SSC [Thermo, AM9765]), and then resuspended in 50 μL RNA FISH hybridization buffer (30% v/v formamide, 10% w/v dextran 15 sulfate [Millipore, S4030], and 1 mg/mL yeast tRNA [Thermo, 15401029] in 2×SSC) supplemented with the appropriate rRNA probe at a final concentration of 8 μM. ..

    Article Title: Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis.
    Article Snippet: Cells were post-fixed using 5 mM Bis(NHS)PEG5 (803537; Sigma-Aldrich) in PBS for 30 min at RT to preserve cell fixation during primary FISH library hybridization at 37°C. .. Hybridization of primary FISH probe libraries targeting 1.2 Mb regions (Chr14 50.92–52.10 Mb, Chr5 149.50–150.70 Mb, Chr2 191.11–192.31 Mb) with 12 kb genomic resolution (one trace-spot = tiled set of ∼150 FISH probes with common docking handle) was performed by incubation with hybridization buffer (50% formamide [FA, AM9342; Thermo Fisher Scientific], 10% [wt/vol] dextran sulfate [D8906; SigmaAldrich] in 2xSSC [AM9763; Thermo Fisher Scientific]) containing the FISH probe libraries at a final concentration of 100–200 ng/μl DNA per library for one to two nights at 37°C in a humidified chamber. ..

    Concentration Assay:

    Article Title: Highly Multiplexed Spatial Transcriptomics in Bacteria
    Article Snippet: .. 10 50X Expansion for Liquid Culture To hybridize the rRNA probes, permeabilized cells were washed twice in 1 mL RNA FISH wash buffer (30% v/v formamide [Thermo, AM9342] in 2×SSC [Thermo, AM9765]), and then resuspended in 50 μL RNA FISH hybridization buffer (30% v/v formamide, 10% w/v dextran 15 sulfate [Millipore, S4030], and 1 mg/mL yeast tRNA [Thermo, 15401029] in 2×SSC) supplemented with the appropriate rRNA probe at a final concentration of 8 μM. ..

    Article Title: Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis.
    Article Snippet: Cells were post-fixed using 5 mM Bis(NHS)PEG5 (803537; Sigma-Aldrich) in PBS for 30 min at RT to preserve cell fixation during primary FISH library hybridization at 37°C. .. Hybridization of primary FISH probe libraries targeting 1.2 Mb regions (Chr14 50.92–52.10 Mb, Chr5 149.50–150.70 Mb, Chr2 191.11–192.31 Mb) with 12 kb genomic resolution (one trace-spot = tiled set of ∼150 FISH probes with common docking handle) was performed by incubation with hybridization buffer (50% formamide [FA, AM9342; Thermo Fisher Scientific], 10% [wt/vol] dextran sulfate [D8906; SigmaAldrich] in 2xSSC [AM9763; Thermo Fisher Scientific]) containing the FISH probe libraries at a final concentration of 100–200 ng/μl DNA per library for one to two nights at 37°C in a humidified chamber. ..

    Incubation:

    Article Title: Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis.
    Article Snippet: Cells were post-fixed using 5 mM Bis(NHS)PEG5 (803537; Sigma-Aldrich) in PBS for 30 min at RT to preserve cell fixation during primary FISH library hybridization at 37°C. .. Hybridization of primary FISH probe libraries targeting 1.2 Mb regions (Chr14 50.92–52.10 Mb, Chr5 149.50–150.70 Mb, Chr2 191.11–192.31 Mb) with 12 kb genomic resolution (one trace-spot = tiled set of ∼150 FISH probes with common docking handle) was performed by incubation with hybridization buffer (50% formamide [FA, AM9342; Thermo Fisher Scientific], 10% [wt/vol] dextran sulfate [D8906; SigmaAldrich] in 2xSSC [AM9763; Thermo Fisher Scientific]) containing the FISH probe libraries at a final concentration of 100–200 ng/μl DNA per library for one to two nights at 37°C in a humidified chamber. ..



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    Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) <t>Melanophore</t> <t>pigmentation</t> defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.
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    Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) <t>Melanophore</t> <t>pigmentation</t> defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.
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    Image Search Results


    Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) Melanophore pigmentation defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.

    Journal: Molecular Biology and Evolution

    Article Title: Genetic and Developmental Divergence in the Neural Crest Program between Cichlid Fish Species

    doi: 10.1093/molbev/msae217

    Figure Lengend Snippet: Paralog-specific KO phenotypes indicate functional divergence of sox10a and sox10b in cichlids. a) Melanophore pigmentation defects, particularly decreased abundance, are observed in sox10b but not in sox10a A. calliptera mutants. b) sox10b -CRISPR embryos have significantly reduced melanophore counts at 7 dpf compared with WT and control injections (boxplots showing melanophore count within the dorsal head area outlined in a.; P -values are shown for Tukey HSD). c) Representative craniofacial phenotypes of sox10a- CRISPR embryos in live animals and their corresponding cartilage preparations in lateral and ventral views. White dashed lines show the reduced frontal slope of the brain case. Arrowheads and asterisks in panels showing cartilage preparations indicate reduced and missing cartilages, respectively, in the mutants. Fractions indicate the frequency of presented phenotype across all surviving embryos on that day. Data shown are representative of at least biological replicates per target gene. Ba, branchial arches; bhy, basihyal; dpf, days postfertilization; op, orbital process; pf, pectoral fin; soc, super-orbital cartilages; WT, wild-type. Scale bars = 1 mm.

    Article Snippet: Embryos were stained for cartilage following the protocol of with following modifications: (i) all specimens were bleached to remove melanophore pigmentation using a solution of 0.05% hydrogen peroxide (Sigma) and 0.05% formamide (ThermoFisher) for 30 to 45 min under light and (ii) samples were cleared using first 50% then 70% glycerol:water solutions until complete sinking.

    Techniques: Functional Assay, CRISPR, Control