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formaldehyde solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher formaldehyde solution
    Formaldehyde Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/formaldehyde+solution/Formaldehyde%2C+4%25+in+PBS/pm42329475-58-18-21
    Average 99 stars, based on 1 article reviews
    formaldehyde solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Scalable hypothalamic neuron differentiation from human pluripotent stem cells suitable for modeling metabolic disorders.
    Article Snippet: Immunocytochemistry and high content imaging analysis hPSC-derived hypothalamic neurons at different time points were cultured as described above on glass-bottom multiwell plates (Cellvis, P24-1.5H-N). .. The cultures were fixed with 4% formaldehyde (Thermo-Fisher, 28908) in PBS for 15 min. Then the cultures were incubated with PBS +4% donkey serum +0.1% Triton X- for 1 h at room temperature, followed by incubation with primary antibodies overnight at 4 ◦ C. Secondary antibodies were incubated at room temperature for 1 h. Refer to Table S1 for complete list of primary and secondary antibodies. .. The cultures were stained with 4 μM Hoechst 33342 (VWR, PI62249) in PBS for 10 min before imaging on the Opera Phenix high-content microscope (Revvity) or Zeiss LSM 710 confocal microscope.

    Staining:

    Article Title: Cell size-dependent mRNA transcription drives proteome remodeling.
    Article Snippet: Cells were induced with doxycycline for 4 or 5 days then seeded onto 35mm imaging dishes (MatTek P35G-1.5-20-C). .. After 2 and 3 days of growth, cells were fixed with 4% formaldehyde (Thermo Scientific; 28906), and after washing twice with PBS, stained with Hoechst33342 (Thermo Scientific; 62249) at 20 μM. ..

    Membrane:

    Article Title: Mitochondrial integrated stress response activation creates a therapeutic vulnerability to MCL-1 inhibition in acute myeloid leukemia.
    Article Snippet: Briefly, 500,000 cells were seeded and treated with either DMSO or 50 nM IACS for 24 h. Cells were collected, washed with PBS and stained with AquaZombie viability dye (Biolegend). .. Cells were exposed to a 0.0001% digitonin (Thermo Fisher Scientific) for membrane permeabilization and then exposed to concentrations increasing up to 5 M of human BIM peptide (sequence: MRPEIWIAQELRRIGDEFNA) for 1 h at 23 C. After BIM peptide exposure, 4% formaldehyde in PBS (Thermo Fisher Scientific) was added to terminate the peptide exposure, followed by neutralization with N2 buffer (1.7M Tris base, 1.25M glycine, pH 9.1). .. The cells were stained with 1:40 with anti-cytochrome c antibody (clone 6H2.B4, Biolegend) overnight.

    Sequencing:

    Article Title: Mitochondrial integrated stress response activation creates a therapeutic vulnerability to MCL-1 inhibition in acute myeloid leukemia.
    Article Snippet: Briefly, 500,000 cells were seeded and treated with either DMSO or 50 nM IACS for 24 h. Cells were collected, washed with PBS and stained with AquaZombie viability dye (Biolegend). .. Cells were exposed to a 0.0001% digitonin (Thermo Fisher Scientific) for membrane permeabilization and then exposed to concentrations increasing up to 5 M of human BIM peptide (sequence: MRPEIWIAQELRRIGDEFNA) for 1 h at 23 C. After BIM peptide exposure, 4% formaldehyde in PBS (Thermo Fisher Scientific) was added to terminate the peptide exposure, followed by neutralization with N2 buffer (1.7M Tris base, 1.25M glycine, pH 9.1). .. The cells were stained with 1:40 with anti-cytochrome c antibody (clone 6H2.B4, Biolegend) overnight.

    Neutralization:

    Article Title: Mitochondrial integrated stress response activation creates a therapeutic vulnerability to MCL-1 inhibition in acute myeloid leukemia.
    Article Snippet: Briefly, 500,000 cells were seeded and treated with either DMSO or 50 nM IACS for 24 h. Cells were collected, washed with PBS and stained with AquaZombie viability dye (Biolegend). .. Cells were exposed to a 0.0001% digitonin (Thermo Fisher Scientific) for membrane permeabilization and then exposed to concentrations increasing up to 5 M of human BIM peptide (sequence: MRPEIWIAQELRRIGDEFNA) for 1 h at 23 C. After BIM peptide exposure, 4% formaldehyde in PBS (Thermo Fisher Scientific) was added to terminate the peptide exposure, followed by neutralization with N2 buffer (1.7M Tris base, 1.25M glycine, pH 9.1). .. The cells were stained with 1:40 with anti-cytochrome c antibody (clone 6H2.B4, Biolegend) overnight.



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