control fopflash luciferase reporter plasmid constructs (Addgene inc)
Addgene inc is a verified supplier 
94
Structured Review
Addgene inc
control fopflash luciferase reporter plasmid constructs
Control Fopflash Luciferase Reporter Plasmid Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fopflash+construct/M51+Super+8x+FOPFlash+(TOPFlash+mutant)+(Plasmid+%2312457)/us10526616-207-7-13
Average 94 stars, based on 258 article reviews
Control Fopflash Luciferase Reporter Plasmid Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fopflash+construct/M51+Super+8x+FOPFlash+(TOPFlash+mutant)+(Plasmid+%2312457)/us10526616-207-7-13
Average 94 stars, based on 258 article reviews
control fopflash luciferase reporter plasmid constructs - by Bioz Stars,
2026-10
94/100 stars
Images
Related Articles
Transfection:Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: .. HEK293T cells were transfected with M50 Super 8x TOPFlash (Addgene #12456) or the Article Title: Wnt5a Regulates Focal Adhesion Formation to Promote Migration in Ewing Sarcoma Article Snippet: Quantification of gene expression was performed by the ΔΔCt method, normalized to the housekeeping gene B2M, and quality controls including the Reverse Transcription Control assay (Bio-Rad), DNA Contamination Control assay (Bio-Rad), and RNA Quality assay (Bio-Rad) were all performed per manufacturer’s instructions. .. Cells were seeded in a 24-well plate in triplicates, and were transfected using Lipofectamine 2000 (#11668027, Invitrogen, Life Technology, Carlsbad, CA, USA) with either M50 Super 8× TOPFlash (Addgene plasmid #12456, Watertown, MA, USA) or Article Title: NR3C1 Modulates Wnt Signalling to Influence the Invasiveness and Immune Features of Nonfunctioning Invasive Pituitary Adenomas. Article Snippet: .. Cells were co- transfected using Lipofectamine 3000 (Thermo Fisher, L3000015) with either TOPflash (Addgene, #12456) or Article Title: The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathway Article Snippet: The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure the activities of firefly luciferase and renilla luciferase in the cell lysates collected 24 h post-transfection, according to the manufacturer’s instructions. .. For the Wnt/β-catenin reporter assay in primary oviductal epithelial cells, primary oviductal epithelial cells were transfected with TOPflash (Addgene, catalog no. 12456, USA) or Article Title: The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathway. Article Snippet: The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure the activities of firefly luciferase and renilla luciferase in the cell lysates collected 24 h post-transfection, according to the manufacturer’s instructions. .. For the Wnt/β-catenin reporter assay in primary oviductal epithelial cells, primary oviductal epithelial cells were transfected with TOPflash (Addgene, catalog no. 12456, USA) or Control:Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: .. HEK293T cells were transfected with M50 Super 8x TOPFlash (Addgene #12456) or the Article Title: NR3C1 Modulates Wnt Signalling to Influence the Invasiveness and Immune Features of Nonfunctioning Invasive Pituitary Adenomas. Article Snippet: .. Cells were co- transfected using Lipofectamine 3000 (Thermo Fisher, L3000015) with either TOPflash (Addgene, #12456) or Plasmid Preparation:Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: .. HEK293T cells were transfected with M50 Super 8x TOPFlash (Addgene #12456) or the Article Title: Wnt5a Regulates Focal Adhesion Formation to Promote Migration in Ewing Sarcoma Article Snippet: Quantification of gene expression was performed by the ΔΔCt method, normalized to the housekeeping gene B2M, and quality controls including the Reverse Transcription Control assay (Bio-Rad), DNA Contamination Control assay (Bio-Rad), and RNA Quality assay (Bio-Rad) were all performed per manufacturer’s instructions. .. Cells were seeded in a 24-well plate in triplicates, and were transfected using Lipofectamine 2000 (#11668027, Invitrogen, Life Technology, Carlsbad, CA, USA) with either M50 Super 8× TOPFlash (Addgene plasmid #12456, Watertown, MA, USA) or Article Title: Phosphoglycerate kinase 1 stimulates β-catenin signaling, which is further enhanced by the transfection of bovine alphaherpesvirus 1 latency-related protein ORF2 Article Snippet: Bovine alphaherpesvirus 1 (BoAHV-1) mainly infects cattle and causes substantial economic losses to the cattle industry worldwide.. The virus establishes a lifelong latent infection primarily in the trigeminal ganglia (TG), where the open reading frame 2 (ORF2), a product of viral latency-related (LR) gene is abundantly expressed.. ORF2 has anti-apoptotic effects, in part by upregulating β-catenin signaling. Construct:Article Title: Wnt5a Regulates Focal Adhesion Formation to Promote Migration in Ewing Sarcoma Article Snippet: Quantification of gene expression was performed by the ΔΔCt method, normalized to the housekeeping gene B2M, and quality controls including the Reverse Transcription Control assay (Bio-Rad), DNA Contamination Control assay (Bio-Rad), and RNA Quality assay (Bio-Rad) were all performed per manufacturer’s instructions. .. Cells were seeded in a 24-well plate in triplicates, and were transfected using Lipofectamine 2000 (#11668027, Invitrogen, Life Technology, Carlsbad, CA, USA) with either M50 Super 8× TOPFlash (Addgene plasmid #12456, Watertown, MA, USA) or Reporter Assay:Article Title: The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathway Article Snippet: The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure the activities of firefly luciferase and renilla luciferase in the cell lysates collected 24 h post-transfection, according to the manufacturer’s instructions. .. For the Wnt/β-catenin reporter assay in primary oviductal epithelial cells, primary oviductal epithelial cells were transfected with TOPflash (Addgene, catalog no. 12456, USA) or Article Title: The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathway. Article Snippet: The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure the activities of firefly luciferase and renilla luciferase in the cell lysates collected 24 h post-transfection, according to the manufacturer’s instructions. .. For the Wnt/β-catenin reporter assay in primary oviductal epithelial cells, primary oviductal epithelial cells were transfected with TOPflash (Addgene, catalog no. 12456, USA) or Binding Assay:Article Title: B4GALT1 and Wntless collaborate to block LRP5/6 translocation from Golgi to cell surface. Article Snippet: After translation, low-density lipoprotein receptor–related protein 5/6, LRP5/6, are transported from ER through Golgi to cell surface, where they serve as the co-receptors of Wnt proteins to elicit the WNT/β-catenin signaling.. Here, Golgi-resident β-1, 4galactosyltransferase B4GALT1 is revealed to interact with LRP5/6, causing the Golgi retention of LRP5/6 and ultimately reducing LRP5/6 on the cell surface.. In addition to LRP5/6, B4GALT1 can also bind to the exclusive Wnt transporter Wntless. Negative Control:Article Title: B4GALT1 and Wntless collaborate to block LRP5/6 translocation from Golgi to cell surface. Article Snippet: After translation, low-density lipoprotein receptor–related protein 5/6, LRP5/6, are transported from ER through Golgi to cell surface, where they serve as the co-receptors of Wnt proteins to elicit the WNT/β-catenin signaling.. Here, Golgi-resident β-1, 4galactosyltransferase B4GALT1 is revealed to interact with LRP5/6, causing the Golgi retention of LRP5/6 and ultimately reducing LRP5/6 on the cell surface.. In addition to LRP5/6, B4GALT1 can also bind to the exclusive Wnt transporter Wntless. |
