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fop flash luciferase reporter vector  (BioVector NTCC)

 
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    BioVector NTCC fop flash luciferase reporter vector
    Fop Flash Luciferase Reporter Vector, supplied by BioVector NTCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fop-flash+vector/top+flash+luciferase+reporter+vector/pmc06010684-63-4-20
    Average 90 stars, based on 1 article reviews
    fop flash luciferase reporter vector - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Luciferase:

    Article Title: Long non-coding RNA CDKN2B antisense RNA 1 gene inhibits Gemcitabine sensitivity in bladder urothelial carcinoma
    Article Snippet: .. The TOP Flash and FOP FLASH luciferase reporter vectors which contain wild-type or mutant TCF binding sites were purchased from Biovector NTCC Ltd (Beijing, China). ..

    Mutagenesis:

    Article Title: Long non-coding RNA CDKN2B antisense RNA 1 gene inhibits Gemcitabine sensitivity in bladder urothelial carcinoma
    Article Snippet: .. The TOP Flash and FOP FLASH luciferase reporter vectors which contain wild-type or mutant TCF binding sites were purchased from Biovector NTCC Ltd (Beijing, China). ..

    Binding Assay:

    Article Title: Long non-coding RNA CDKN2B antisense RNA 1 gene inhibits Gemcitabine sensitivity in bladder urothelial carcinoma
    Article Snippet: .. The TOP Flash and FOP FLASH luciferase reporter vectors which contain wild-type or mutant TCF binding sites were purchased from Biovector NTCC Ltd (Beijing, China). ..

    other:

    Article Title: Non-coding RNA NEAT1/miR-214-3p contribute to doxorubicin resistance of urothelial bladder cancer preliminary through the Wnt/β-catenin pathway
    Article Snippet: The Wnt signaling quantitation luciferase reporter plasmids (TOP Flash, FOP Flash) were purchased from BioVector NTCC Inc. (Beijing, People’s Republic of China).

    Article Title: Long non-coding RNA CCAT2 promotes cell proliferation and invasion through regulating Wnt/β-catenin signaling pathway in clear cell renal cell carcinoma.
    Article Snippet: ACHN cells were seeded in 24-well plates and co-transfected with the TOP/FOP Flash expression plasmids (Biovector NTCC Ltd., Beijing, China) and Renilla TK-luciferase vector (Promega Corp., Madison, WI, USA) as a control; 48 h after transfection, the cells were harvested and lysed for a luciferase assay.

    Article Title: Silencing of long non-coding RNA CCAT2 depressed malignancy of oral squamous cell carcinoma via Wnt/β-catenin pathway.
    Article Snippet: The TOP Flash (wild type (wt) TCF binding sites, Catalog # 21-170) luciferase reporter plasmid along with FOP Flash (mutant TCF binding sites, Catalog # 21-169) purchased from Biovector NTCC Ltd (Beijing, China) enables quantitation of Wnts signaling in cells transfected with these constructs.

    Article Title: SNHG1/miR-556-5p/TCF12 feedback loop enhances the tumorigenesis of meningioma through Wnt signaling pathway.
    Article Snippet: Funding information Anhui colleges and universities natural science research project (Key project), Grant/Award Number: KJ2018A0812 Abstract Meningioma, as a sort of the malignantly intracranial tumors, has captured public attention for its second‐highest morbidity all over the world.. Long noncoding RNAs (lncRNAs), including lncRNA SNHG1, have been well known as essential players in the development of diverse cancers.. However, the biological effect and regulatory mechanism of SNHG1 have not been mentioned in meningioma.



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    The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined <t>by</t> <t>TOP</t> <t>Flash/FOP</t> Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.
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    EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d <t>TOP/FOP</t> flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01
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    Image Search Results


    The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined by TOP Flash/FOP Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.

    Journal: Heliyon

    Article Title: Hyperthermia improves gemcitabine sensitivity of pancreatic cancer cells by suppressing the EFNA4/β-catenin axis and activating dCK

    doi: 10.1016/j.heliyon.2024.e28488

    Figure Lengend Snippet: The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined by TOP Flash/FOP Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.

    Article Snippet: To assess the β-catenin-mediated transcriptional activity of TCF/LEF, the TOP Flash reporter vector (D2501, Beyotime) was utilized, with the FOP Flash reporter vector containing mutant TCF/LEF binding sequences (D2503, Beyotime) serving as the NC.

    Techniques: Activation Assay, Activity Assay, Luciferase, Reporter Assay, Over Expression, Comparison

    EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d TOP/FOP flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01

    Journal: Biology Direct

    Article Title: H3K27ac-activated EGFR-AS1 promotes cell growth in cervical cancer through ACTN4-mediated WNT pathway

    doi: 10.1186/s13062-021-00315-5

    Figure Lengend Snippet: EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d TOP/FOP flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01

    Article Snippet: Besides, TOP/FOP-flash reporter vectors were available to assay the Wnt/β-catenin signaling activity as guided by manufacturer (Addgene, Cambridge, MA).

    Techniques: Quantitative RT-PCR, Expressing, Transfection, Translocation Assay, Fractionation, Activity Assay, Western Blot, Flow Cytometry