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Structured Review

Promega top/fop-flash vectors
Top/Fop Flash Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fop+flash+vector/t+vectors/pmc08343583-58-5-7
Average 90 stars, based on 1 article reviews
top/fop-flash vectors - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Sequencing:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Clone Assay:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Construct:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Binding Assay:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Plasmid Preparation:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Transfection:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Luciferase:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Derivative Assay:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Activity Assay:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Standard Deviation:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Transduction:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Expressing:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.

Transformation Assay:

Article Title: Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance
Article Snippet: PCR-amplified products were either directly sent out for sequencing or cloned to T-vectors (Promega, Madison, WI, USA) before sequencing (Sangon Biotechnology, Shanghai, China).

Article Title: Evidence of Purifying Selection and Co-Evolution at the Fold-Back Arm of the Novel Precursor MicroRNA159 Gene in Phalaenopsis Species (Orchidaceae)
Article Snippet: Recovered PCR products were ligated into T-vectors (Promega, Wisconsin, USA), and recombinants were transformed into Escherichia coli DH5α (RBC, Taipei, Taiwan).

Article Title: Lycopene inhibits NF-κB activation and adhesion molecule expression through Nrf2-mediated heme oxygenase-1 in endothelial cells.
Article Snippet: The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22).

Article Title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
Article Snippet: PCR products were subcloned into T-vectors (Promega, Madison, WI, U.S.A.).

Article Title: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Article Snippet: The PCR products were cloned into T-vectors (Promega, WI, USA) and individually sequenced with primers for the promoter regions of OCT4 (POU5F1) , NANOG , and LIN28A .

Article Title: MicroRNA-934 promotes colorectal cancer cell proliferation by directly targeting Dickkopf-related protein 2
Article Snippet: BCA Protein Assay kit (cat. no. 23225; Thermo Fisher Scientific, Inc.) was used to determine the protein concentration.

Article Title: Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy
Article Snippet: Finally, the PCR products were cloned into T-vectors (Promega, Madison, WI, USA), and individual clones were sequenced by Genotech ( http://www.genotech.co.kr , Daejeon, Korea).

Article Title: Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing
Article Snippet: 293FT cells were seeded at 25,000 cells per well in a 96-well plate and were transfected using Lipofectamine LTX with 10 ng of pCMV/MLV/Luc or pCMV/dl587/Luc, 10 ng of a TK promoter-driven Renilla luciferase control plasmid vector (pGL4.74, Promega), and 45 ng of one of the pCMV/flag-X vectors.



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Image Search Results


The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined by TOP Flash/FOP Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.

Journal: Heliyon

Article Title: Hyperthermia improves gemcitabine sensitivity of pancreatic cancer cells by suppressing the EFNA4/β-catenin axis and activating dCK

doi: 10.1016/j.heliyon.2024.e28488

Figure Lengend Snippet: The suppressive effect of EFNA4 on dCK depends on the β-catenin signaling activation. A, protein level of β-catenin in in BxPC-3/GEM and PANC-1/GEM cells after hyperthermia examined by WB analysis (uncropped images of blots are provided as Supplementary material); B, transcriptional activity of β-catenin in cells after hyperthermia determined by TOP Flash/FOP Flash luciferase reporter assay; C, transcriptional activity of β-catenin in cells after EFNA4 overexpression and MSAB treatment determined by TOP Flash/FOP Flash luciferase reporter assay; D, protein levels of β-catenin and dCK in cells after EFNA4 overexpression and MSAB treatment determined by WB analysis (uncropped images of blots are provided as Supplementary material). Three biological replicates were performed. Differences were compared by the two-way ANOVA followed by Sidak's (A–B) or Tukey's (C–D) multiple comparison test. * p < 0.05.

Article Snippet: To assess the β-catenin-mediated transcriptional activity of TCF/LEF, the TOP Flash reporter vector (D2501, Beyotime) was utilized, with the FOP Flash reporter vector containing mutant TCF/LEF binding sequences (D2503, Beyotime) serving as the NC.

Techniques: Activation Assay, Activity Assay, Luciferase, Reporter Assay, Over Expression, Comparison

EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d TOP/FOP flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01

Journal: Biology Direct

Article Title: H3K27ac-activated EGFR-AS1 promotes cell growth in cervical cancer through ACTN4-mediated WNT pathway

doi: 10.1186/s13062-021-00315-5

Figure Lengend Snippet: EGFR-AS1 activates ACTN4-mediated WNT pathway in CC cells. a The knockdown efficacy of sh-ACTN4#1/2 on was tested by qRT-PCR in CC cells. b Expression levels of WNT pathway downstream genes in sh-ACTN4#1 transfected cells were detected by qRT-PCR in CC cells. c The effect of ACTN4 deficiency on the nuclear translocation of CTNNB1 (β-catenin) was detected by subcellular fractionation assay. d TOP/FOP flash assay was performed to measure the activity of WNT pathway upon ACTN4 knockdown. e , f Detection of mRNA and protein levels of WNT pathway downstream genes in CC cells transfected with sh-EGFR-AS1#1 was detected by qRT-PCR and western blot assays. g , h The nuclear translocation of CTNNB1 (β-catenin) was observed in sh-EGFR-AS1#1 group and sh-NC group. i Impact of EGFR-AS1 silencing on WNT pathway activity was detected. j – l Cell proliferation, apoptosis and invasion in cell transfected with sh-NC, sh-EGFR-AS1#1 and sh-EGFR-AS1#1 + LiCl were assessed by EdU, flow cytometry and transwell assays. **P < 0.01

Article Snippet: Besides, TOP/FOP-flash reporter vectors were available to assay the Wnt/β-catenin signaling activity as guided by manufacturer (Addgene, Cambridge, MA).

Techniques: Quantitative RT-PCR, Expressing, Transfection, Translocation Assay, Fractionation, Activity Assay, Western Blot, Flow Cytometry