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fluoroshield tm with dapi  (Beyotime)


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    Structured Review

    Beyotime fluoroshield tm with dapi
    Fluoroshield Tm With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshield+tm+dapi/DAPI/pmc11237085-260-63-67
    Average 99 stars, based on 33153 article reviews
    fluoroshield tm with dapi - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: SATB1 lowers the T cell receptor signaling threshold for thymocyte positive selection via super-enhancer reprogramming.
    Article Snippet: Followed by 3 × PBS wash, followed by incubation with a secondary antibody in conjunctionwithAlexa 647fluorescence (FD0144) 1:1000 in 1 × PBS for onehour in the dark. .. Afterwashing three times, the nuclei were stained with 10 μg/ml DAPI (Beyotime). .. Images were acquired by a confocal microscope (Carl Zeiss, LSM 880 with Airyscan or Nikon A1 R) with a 100x objective.

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    Blocking Assay:

    Article Title: NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X‐100 for 10 min. After blocking with 5% BSA for 1 h, cells were incubated with an anti‐NPM1 antibody (Abcam, UK) overnight at 4°C, followed by incubation with an Alexa Fluor‐conjugated secondary antibody for 1 h. Nuclei were counterstained with DAPI (Beyotime, China). ..

    Incubation:

    Article Title: NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X‐100 for 10 min. After blocking with 5% BSA for 1 h, cells were incubated with an anti‐NPM1 antibody (Abcam, UK) overnight at 4°C, followed by incubation with an Alexa Fluor‐conjugated secondary antibody for 1 h. Nuclei were counterstained with DAPI (Beyotime, China). ..

    Article Title: Lineage-specific and gestational age–dependent DNA methylation programming orchestrates human placental development
    Article Snippet: Then, they were incubated with 5mC (61255, Active Motif), EGFR (ab30, Abcam), HLA-G (ab52454, Abcam), TFAP2C (sc12762, Santa Cruz Biotechnology), CDH2 (ab18203, Abcam), EpCAM (MA5-12436, Thermo Fisher Scientific), TP63 (ab124762, Abcam), SDC1 (67155-1-Ig, Proteintech), CGB (ab9582, Abcam), or α-SMA (MA1-06110, Thermo Fisher Scientific) overnight at 4°C. .. After multiple washes to remove residual primary antibody, cells or sections were then incubated with AF488/AF594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 hour at room temperature and mounted in prolonged anti-fade reagent with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime). .. Confocal imaging was performed with Zeiss LSM 880 confocal microscopes or LEICA TCS SP8, and analyzed with Zeiss Zen blue edition.

    TUNEL Assay:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Labeling:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Fluorescence:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Microscopy:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    BIA-KA:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    Protein Concentration:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    LDH Cytotoxicity Assay:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).



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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with <t>DAPI</t> (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.
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    Image Search Results


    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Article Snippet: The specimens were washed with PBS and mounted in DAPI anti-fade aqueous medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific).

    Techniques: Expressing

    E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Article Snippet: The specimens were washed with PBS and mounted in DAPI anti-fade aqueous medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific).

    Techniques: Expressing

    ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: ZO-1 immunolabelling in the ileal mucosa. ( a – e ) Representative images of ZO-1 expression (green). Nuclei are counterstained with DAPI (blue). ( f ) Densitometric analysis of ZO-1-positive area. Exposure to hyperoxia significantly decreased ZO-1 expression compared to the normoxic controls ( a , c , f ). The treatment with BRL37344 at 3 mg/kg, but not at 1 mg/kg, had a protective effect ( c – f ). No significant changes were observed in the normoxia-exposed pups, treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§§ p < 0.001 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 50 µm.

    Article Snippet: After the final washing step with PBS, the strips were transferred into a droplet of the anti-fade DAPI mounting medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific, Waltham, MA, USA) dripped onto the microscope slide and oriented on their side to visualize the villus axis [ ].

    Techniques: Expressing

    E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Journal: Antioxidants

    Article Title: Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations

    doi: 10.3390/antiox13070863

    Figure Lengend Snippet: E-cadherin immunolabelling in the ileal mucosa. ( a – e ) Representative images of E-cadherin expression (green; nuclei are counterstained blue with DAPI). ( f ) Densitometric analysis of E-cadherin-positive area. Exposure to hyperoxia significantly decreased E-cadherin expression compared to the normoxic controls ( a , c , f ). The administration of BRL37344 had a partially protective effect ( c – f ). No changes can be observed in the normoxic pups treated or not with BRL37344 ( a , b , f ). Values are expressed as mean ± S.D. *** p < 0.001 hyperoxia versus normoxia; °° p < 0.01 and °°° p < 0.001 hyperoxia + BRL37344 versus normoxia; §§ p < 0.01 hyperoxia + BRL37344 versus hyperoxia. Scale bar = 25 µm.

    Article Snippet: After the final washing step with PBS, the strips were transferred into a droplet of the anti-fade DAPI mounting medium (Fluoroshield TM with DAPI, Thermo Fisher Scientific, Waltham, MA, USA) dripped onto the microscope slide and oriented on their side to visualize the villus axis [ ].

    Techniques: Expressing