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ImmunoBiosciences Inc fluoroshield with dapi
UMAP projections showing the expression of a HLA-I and HLA-II in the CD45 − fraction. HLA-I and II expression was measured by summing the expression of all the individual genes assessed in the locus. b Comparison of HLA-II and CD74 (invariant chain) expression ( R = 0.751, unadjusted p = 0, Pearson correlation test). c Boxplots show the log-transformed expression of the individual HLA-II genes assessed ( p = 1.01 × 10 −178 , Kruskal–Wallis, n = 1377 cells). The box represents the interquartile range, with the center line at the median. The whiskers extend up to 1.5 times the interquartile range (IQR). d Representative multiplex immunofluorescent staining of CD8 (blue), CD4 (red), GZMB (green), PanCK (magenta), HLA-DR (cyan), and <t>DAPI</t> (gray) in an NSCLC tumor ( n = 2). Overlay without DAPI is shown for CD8, CD4, and GZMB, and for PanCK and HLA-DR, along with the corresponding H&E. Scale bar, 25 μm. Source data are provided as a Source Data file.
Fluoroshield With Dapi, supplied by ImmunoBiosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluoroshield+dapi/fluoroshield+with+dapi/pmc09279502-311-5-8
Average 90 stars, based on 1 article reviews
fluoroshield with dapi - by Bioz Stars, 2026-09
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1) Product Images from "Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer"

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer

Journal: Nature Communications

doi: 10.1038/s41467-022-31769-4

UMAP projections showing the expression of a HLA-I and HLA-II in the CD45 − fraction. HLA-I and II expression was measured by summing the expression of all the individual genes assessed in the locus. b Comparison of HLA-II and CD74 (invariant chain) expression ( R = 0.751, unadjusted p = 0, Pearson correlation test). c Boxplots show the log-transformed expression of the individual HLA-II genes assessed ( p = 1.01 × 10 −178 , Kruskal–Wallis, n = 1377 cells). The box represents the interquartile range, with the center line at the median. The whiskers extend up to 1.5 times the interquartile range (IQR). d Representative multiplex immunofluorescent staining of CD8 (blue), CD4 (red), GZMB (green), PanCK (magenta), HLA-DR (cyan), and DAPI (gray) in an NSCLC tumor ( n = 2). Overlay without DAPI is shown for CD8, CD4, and GZMB, and for PanCK and HLA-DR, along with the corresponding H&E. Scale bar, 25 μm. Source data are provided as a Source Data file.
Figure Legend Snippet: UMAP projections showing the expression of a HLA-I and HLA-II in the CD45 − fraction. HLA-I and II expression was measured by summing the expression of all the individual genes assessed in the locus. b Comparison of HLA-II and CD74 (invariant chain) expression ( R = 0.751, unadjusted p = 0, Pearson correlation test). c Boxplots show the log-transformed expression of the individual HLA-II genes assessed ( p = 1.01 × 10 −178 , Kruskal–Wallis, n = 1377 cells). The box represents the interquartile range, with the center line at the median. The whiskers extend up to 1.5 times the interquartile range (IQR). d Representative multiplex immunofluorescent staining of CD8 (blue), CD4 (red), GZMB (green), PanCK (magenta), HLA-DR (cyan), and DAPI (gray) in an NSCLC tumor ( n = 2). Overlay without DAPI is shown for CD8, CD4, and GZMB, and for PanCK and HLA-DR, along with the corresponding H&E. Scale bar, 25 μm. Source data are provided as a Source Data file.

Techniques Used: Expressing, Comparison, Transformation Assay, Multiplex Assay, Staining

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Expressing:

Article Title: Reagent compounds, compositions, kits, and methods for amplified assays
Article Snippet: were rinsed with distilled water, removing excess water with paper towel. .. 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat# AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Protocol B— tyramide-hapten staining: Following antigen retrieval the following

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: Ca2+ and Mg2+ for 2 min and incubated with Alexa Fluor® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca2+ and Mg2+. .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: 2+ and Mg 2+ for 2 min and incubated with Alexa Fluor ® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca 2+ and Mg 2+ . .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Antigen-coupled immunoreagents
Article Snippet: 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat#AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Tri

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer
Article Snippet: and added to the slide and incubated for 1 h. As a negative control, slides were incubated with the secondary anti-Tag detection cocktail alone. .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010) using the Leica Quantitative Algorithm (v1).

Article Title: Single-cell analysis reveals new evolutionary complexity in uveal melanoma
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Comparison:

Article Title: Reagent compounds, compositions, kits, and methods for amplified assays
Article Snippet: were rinsed with distilled water, removing excess water with paper towel. .. 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat# AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Protocol B— tyramide-hapten staining: Following antigen retrieval the following

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: Ca2+ and Mg2+ for 2 min and incubated with Alexa Fluor® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca2+ and Mg2+. .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: 2+ and Mg 2+ for 2 min and incubated with Alexa Fluor ® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca 2+ and Mg 2+ . .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Antigen-coupled immunoreagents
Article Snippet: 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat#AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Tri

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer
Article Snippet: and added to the slide and incubated for 1 h. As a negative control, slides were incubated with the secondary anti-Tag detection cocktail alone. .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010) using the Leica Quantitative Algorithm (v1).

Article Title: Single-cell analysis reveals new evolutionary complexity in uveal melanoma
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Transformation Assay:

Article Title: Reagent compounds, compositions, kits, and methods for amplified assays
Article Snippet: were rinsed with distilled water, removing excess water with paper towel. .. 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat# AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Protocol B— tyramide-hapten staining: Following antigen retrieval the following

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: Ca2+ and Mg2+ for 2 min and incubated with Alexa Fluor® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca2+ and Mg2+. .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
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Article Title: Antigen-coupled immunoreagents
Article Snippet: 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat#AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Tri

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer
Article Snippet: and added to the slide and incubated for 1 h. As a negative control, slides were incubated with the secondary anti-Tag detection cocktail alone. .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010) using the Leica Quantitative Algorithm (v1).

Article Title: Single-cell analysis reveals new evolutionary complexity in uveal melanoma
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Multiplex Assay:

Article Title: Reagent compounds, compositions, kits, and methods for amplified assays
Article Snippet: were rinsed with distilled water, removing excess water with paper towel. .. 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat# AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Protocol B— tyramide-hapten staining: Following antigen retrieval the following

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: Ca2+ and Mg2+ for 2 min and incubated with Alexa Fluor® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca2+ and Mg2+. .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: 2+ and Mg 2+ for 2 min and incubated with Alexa Fluor ® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca 2+ and Mg 2+ . .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Antigen-coupled immunoreagents
Article Snippet: 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat#AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Tri

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer
Article Snippet: and added to the slide and incubated for 1 h. As a negative control, slides were incubated with the secondary anti-Tag detection cocktail alone. .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010) using the Leica Quantitative Algorithm (v1).

Article Title: Single-cell analysis reveals new evolutionary complexity in uveal melanoma
Article Snippet: were then washed with wash buffer (3 × 5 min) and rinsed with distilled water (1 × 2 min). .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20X using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010), using Leica Quantitative Algorit

Staining:

Article Title: Reagent compounds, compositions, kits, and methods for amplified assays
Article Snippet: were rinsed with distilled water, removing excess water with paper towel. .. 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat# AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Protocol B— tyramide-hapten staining: Following antigen retrieval the following

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: Ca2+ and Mg2+ for 2 min and incubated with Alexa Fluor® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca2+ and Mg2+. .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: 2+ and Mg 2+ for 2 min and incubated with Alexa Fluor ® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca 2+ and Mg 2+ . .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Antigen-coupled immunoreagents
Article Snippet: 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat#AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Tri

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer
Article Snippet: and added to the slide and incubated for 1 h. As a negative control, slides were incubated with the secondary anti-Tag detection cocktail alone. .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010) using the Leica Quantitative Algorithm (v1).

Article Title: Single-cell analysis reveals new evolutionary complexity in uveal melanoma
Article Snippet: were then washed with wash buffer (3 × 5 min) and rinsed with distilled water (1 × 2 min). .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20X using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010), using Leica Quantitative Algorit

RNA Expression:

Article Title: Reagent compounds, compositions, kits, and methods for amplified assays
Article Snippet: were rinsed with distilled water, removing excess water with paper towel. .. 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat# AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Protocol B— tyramide-hapten staining: Following antigen retrieval the following

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: Ca2+ and Mg2+ for 2 min and incubated with Alexa Fluor® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca2+ and Mg2+. .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: 2+ and Mg 2+ for 2 min and incubated with Alexa Fluor ® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca 2+ and Mg 2+ . .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Antigen-coupled immunoreagents
Article Snippet: 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat#AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Tri

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer
Article Snippet: and added to the slide and incubated for 1 h. As a negative control, slides were incubated with the secondary anti-Tag detection cocktail alone. .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010) using the Leica Quantitative Algorithm (v1).

Article Title: Single-cell analysis reveals new evolutionary complexity in uveal melanoma
Article Snippet: were then washed with wash buffer (3 × 5 min) and rinsed with distilled water (1 × 2 min). .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20X using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010), using Leica Quantitative Algorit

Sequencing:

Article Title: Reagent compounds, compositions, kits, and methods for amplified assays
Article Snippet: were rinsed with distilled water, removing excess water with paper towel. .. 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat# AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Protocol B— tyramide-hapten staining: Following antigen retrieval the following

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: Ca2+ and Mg2+ for 2 min and incubated with Alexa Fluor® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca2+ and Mg2+. .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: 2+ and Mg 2+ for 2 min and incubated with Alexa Fluor ® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca 2+ and Mg 2+ . .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Antigen-coupled immunoreagents
Article Snippet: 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat#AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Tri

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer
Article Snippet: and added to the slide and incubated for 1 h. As a negative control, slides were incubated with the secondary anti-Tag detection cocktail alone. .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010) using the Leica Quantitative Algorithm (v1).

Article Title: Single-cell analysis reveals new evolutionary complexity in uveal melanoma
Article Snippet: were then washed with wash buffer (3 × 5 min) and rinsed with distilled water (1 × 2 min). .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20X using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010), using Leica Quantitative Algorit

Quantitation Assay:

Article Title: Reagent compounds, compositions, kits, and methods for amplified assays
Article Snippet: were rinsed with distilled water, removing excess water with paper towel. .. 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat# AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Protocol B— tyramide-hapten staining: Following antigen retrieval the following

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: Ca2+ and Mg2+ for 2 min and incubated with Alexa Fluor® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca2+ and Mg2+. .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Investigation of the Effect of High Shear Stress on Mesenchymal Stem Cells Using a Rotational Rheometer in a Small-Angle Cone–Plate Configuration
Article Snippet: 2+ and Mg 2+ for 2 min and incubated with Alexa Fluor ® 594 phalloidin (6.6 μM with emission: 609 nm and excitation: 581 nm) for 20 min in the dark. .. Before embedding the cells with Fluoroshield with DAPI (ImmunoBioSciences, Mukilteo, Washington, USA) with emission: 360 nm and excitation: 460 nm, and the cells were washed twice with PBS without Ca 2+ and Mg 2+ . .. Images were taken with an Observer Z1 fluorescence microscope, version1.2 (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen, Germany).

Article Title: Antigen-coupled immunoreagents
Article Snippet: 1-3 drops of Fluoroshield with DAPI (Immunobiosciences, Inc, cat#AR-6501-01) was added to each slide and after 3-5 min in the dark at room temperature the coverslip was applied. .. Tri

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer
Article Snippet: and added to the slide and incubated for 1 h. As a negative control, slides were incubated with the secondary anti-Tag detection cocktail alone. .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010) using the Leica Quantitative Algorithm (v1).

Article Title: Single-cell analysis reveals new evolutionary complexity in uveal melanoma
Article Snippet: were then washed with wash buffer (3 × 5 min) and rinsed with distilled water (1 × 2 min). .. Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20X using the Leica Versa scanner. .. Analysis was performed on the Aperio ImageScope, (v12.4.2.5010), using Leica Quantitative Algorit



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Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with <t>DAPI</t> nuclear counterstain; scale bars = 1 mm and 100 µm (inset).
Fluoroshield With Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

Journal: Frontiers in Dental Medicine

Article Title: Salivary gland transcriptomic analysis and immunophenotyping in the IL-14α transgenic mouse model of Sjögren's disease

doi: 10.3389/fdmed.2025.1612522

Figure Lengend Snippet: Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

Article Snippet: Slides were then washed in PBS and coverslips mounted using Fluoroshield with DAPI (MilliporeSigma).

Techniques: Staining, Immunofluorescence, Marker