cathepsin k assay kit (Novus Biologicals)
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cathepsin k assay kit
Cathepsin K Assay Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/Cathepsin+K+Assay+Kit+(Fluorometric)/pmc13090517-357-10-14
Average 94 stars, based on 1 article reviews
Cathepsin K Assay Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/Cathepsin+K+Assay+Kit+(Fluorometric)/pmc13090517-357-10-14
Average 94 stars, based on 1 article reviews
cathepsin k assay kit - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Siah2 regulates lipid uptake in adipose tissue macrophages"
Article Title: Siah2 regulates lipid uptake in adipose tissue macrophages
Journal: The Journal of Biological Chemistry
doi: 10.1016/j.jbc.2026.111380
Figure Legend Snippet: Lysosomal program is upregulated in ATMs independent of Siah2 . A , relative mRNA expression of lysosomal genes in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. B , representative Western blot of lysosomal enzymes relative to β-actin in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. The β-actin panel is the same as used in F . The blot shown in F was reprobed for the CTSK image in B . C , enzyme activity of CTSK and LAL in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. D and E , relative mRNA expression of Atgl ( D ) and Hilpda ( E ) in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. F , representative Western blot of ATGL and HILPDA relative to β-actin in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. G , basal glycerol release in fl/fl and Siah2 MacKO BMDMs and BM-ATMs treated with vehicle or 50 μM Atglistatin or 0.1 μM Lalistat-1 for 24 h. H , glycerol release in fl/fl and Siah2 MacKO BM-ATMs treated with vehicle or 50 μM Atglistatin or 0.1 μM Lalistat-1 for 24 h with or without 0.5 mM IBMX (activated lipolysis). I and J , representative confocal images ( I ) and quantification ( J ) of fl/fl and Siah2 MacKO BM-ATMs stained for BODIPY 493/503 (LD, green ), LysoTracker (lysosomes, magenta ), and DAPI (nuclei, blue ) showing colocalization of LD and lysosomes. The scale bar represents 10 μm. Red signals of LysoTracker were converted to magenta using the LUT function in ImageJ. Statistics are reported as mean ± SD using an unpaired t test with Welch’s correction, and each dot denotes technical replicates representative of three to five independent experiments. A , D , E , G , H , and J , statistics are reported as mean ± SD using two-way ANOVA with Tukey’s multiple comparisons test ( C ). p Values are indicated on the graphs. ATGL, adipose triglyceride lipase; ATM, adipose tissue macrophage; BM-ATM, bone marrow–derived ATM; BMDM, bone marrow–derived macrophage; CTSK, cathepsin K; DAPI, 4′,6-diamidino-2-phenylindole; fl/fl, Siah2 flox/flox ; HILPDA, hypoxia-inducible LD-associated protein; IBMX, 3-isobutyl-1-methylxanthine; LAL, lysosomal acid lipase; LD, lipid droplet; MacKO, Siah2 MacKO ; Siah2, seven in absentia homolog 2.
Techniques Used: Expressing, Western Blot, Activity Assay, Staining, Derivative Assay
Related Articles
other:Article Title: Siah2 Regulates Lipid Uptake in Adipose Tissue Macrophages. Article Snippet: 100 g of protein extracts were pre-cleared with protein A/G magnetic beads (Pierce, Cat# 88802) for 1 hr at 4°C. Activity Assay:Article Title: Siah2 regulates lipid uptake in adipose tissue macrophages Article Snippet: Nitrocellulose membranes (Bio-Rad, 0.2 μm) were incubated with primary antibodies for 1 to 2 h at room temperature or overnight at 4°C, followed by 1 h incubation with secondary antibody at room temperature. .. CTSK activity in BMDMs and BM-ATMs was measured using the |
