BrdU Cell Proliferation Assay:Article Title: Exposure of carbon nanotubes affects testis and brain of common carp.
Article Snippet: Carbon nanotubes production has been rapidly increasing for many potential applications, however, the environmental impact of this nanomaterial needs to be comprehended.. The present work focused on unraveling the effects of single-walled carbon nanotubes (SWCNT) in the common carp, Cyprinus carpio.. The physicochemical properties of SWCNT were analyzed with X-ray diffraction, Fourier transforms infra-red, UV-Vis absorption, transmission electron microscopy (TEM), and Raman spectroscopy before testing for exposure impact.
Article Title: Common carp pentraxin gene: Evidence for its role in ovarian differentiation and growth.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Chromatin Immunoprecipitation:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
DNA Purification:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
Imaging:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
Conjugation Assay:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
Gas Chromatography-Mass Spectrometry:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
Polymerase Chain Reaction:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
Purification:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
Sample Prep:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
DNA Sequencing:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
RNA Sequencing:Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5
MTT Assay:
Proliferation Assay:
Article Title: Common carp pentraxin gene: Evidence for its role in ovarian differentiation and growth.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Immunofluorescence:Article Title: TMEM219 antibodies and therapeutic uses thereof
Article Snippet: .. Whilst, to determine the level of cell proliferation an immunofluorescence staining of Proliferating cell nuclear antigen (PCNA) was performed using the PCNA assay kit (Novus, NB600-1331) according to the manufacturer's protocol. ..
Staining:Article Title: TMEM219 antibodies and therapeutic uses thereof
Article Snippet: .. Whilst, to determine the level of cell proliferation an immunofluorescence staining of Proliferating cell nuclear antigen (PCNA) was performed using the PCNA assay kit (Novus, NB600-1331) according to the manufacturer's protocol. ..
Control:
|
Novus Biologicals is a verified supplier 
Novus Biologicals manufactures this product 
