fluorescent probes (TIB MOLBIOL)
90
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TIB MOLBIOL
fluorescent probes
Fluorescent Probes, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorogenic+probe/fluorogenic+probes/pm21848694-90-13-32
Average 90 stars, based on 1 article reviews
Fluorescent Probes, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorogenic+probe/fluorogenic+probes/pm21848694-90-13-32
Average 90 stars, based on 1 article reviews
fluorescent probes - by Bioz Stars,
2026-09
90/100 stars
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Polymerase Chain Reaction:Article Title: Methylprednisolone induces apoptosis by interacting with the JAK/STAT pathway in HL-60 and K-562 leukemic cells. Article Snippet: Objective: To determine the gene expression profiles of the JAK/STAT pathway members STAT3, STAT5A, STAT5B at both mRNA and protein levels in HL-60 and K-562 leukemia cells that were undergoing apoptosis following high-dose methylprednisolone (MP) treatment.. Methods: HL-60 cells were treated with 0.1 mM MP and K-562 cells were treated with 0.4 mM MP according to their IC50 values.. STAT3, STAT5A, and STAT5B mRNA relative expression levels were determined by qRTPCR whereas the protein levels were detected via western-blot analysis and apoptosis was evaluated by Annexin V method. Hybridization:Article Title: Methylprednisolone induces apoptosis by interacting with the JAK/STAT pathway in HL-60 and K-562 leukemic cells. Article Snippet: Objective: To determine the gene expression profiles of the JAK/STAT pathway members STAT3, STAT5A, STAT5B at both mRNA and protein levels in HL-60 and K-562 leukemia cells that were undergoing apoptosis following high-dose methylprednisolone (MP) treatment.. Methods: HL-60 cells were treated with 0.1 mM MP and K-562 cells were treated with 0.4 mM MP according to their IC50 values.. STAT3, STAT5A, and STAT5B mRNA relative expression levels were determined by qRTPCR whereas the protein levels were detected via western-blot analysis and apoptosis was evaluated by Annexin V method. Article Title: OPRM1 c.118A>G Polymorphism and Duration of Morphine Treatment Associated with Morphine Doses and Quality-of-Life in Palliative Cancer Pain Settings Article Snippet: .. The reaction mixture (10 μL) contained Fast Start Taq polymerase (10×), buffer and dNTPs, MgCl 2 (10 mM); Lightcycler Fast Start DNA Master Hybridization Probes Kit ® (catalogue no. 03 003 248 001, Roche Diagnostics GmbH), and 0.2 μL of each primer (20 mM) and Article Title: Frequency of CYP2C9 and VKORC1 gene polymorphisms and their influence on warfarin dose in Egyptian pediatric patients. Article Snippet: Introduction Warfarin is a widely used anticoagulant that shows a high inter-individual variability in the dose needed to achieve target anticoagulation.. In adults, common genetic variants in the cytochrome P450-2C9 (CYP2C9) and vitamin K epoxide reductase complex (VKORC1) enzymes, in addition to non-genetic factors, explain this dose variability.. In children, data about warfarin pharmacogenetics are limited and inconsistent. Real-time Polymerase Chain Reaction:Article Title: Nicotinergic Modulation of Attention-Related Neural Activity Differentiates Polymorphisms of DRD2 and CHRNA4 Receptor Genes Article Snippet: .. The polymorphisms in the CHRNA4 and DRD2 genes were genotyped based on the DNA of buccal cells by means of real time PCR (Light Cycler System, Roche Diagnostics, Mannheim, Germany) using Synthesized:Article Title: 2-arachidonoylglycerol signaling in forebrain regulates systemic energy metabolism Article Snippet: .. Primers and Article Title: Genetic low nephron number hypertension is associated with altered expression of osteopontin and CD44 during nephrogenesis. Article Snippet: Aims : The study set out to investigate whether the osteopontin (OPN)-CD44-integrin-receptor-system is differently regulated during nephrogenesis in inborn nephron deficit, a major determinant of human primary hypertension and cardiovascular disease in adult life.. Methods : We compared a genetic rat model with an inherited nephron deficit, the Munich-Wistar-Froemter rat (MWF), to normotensive Wistar rats during nephrogenesis at day 19 of fetal development (E19) and at postpartal day 7 (D7).. Results : Renal OPN mRNA ( – 75 % , P < 0.05) and protein expression ( – 38 % , P < 0.05) were strongly decreased at E19 in MWF compared to Wistar. Article Title: Genetic low nephron number hypertension is associated with altered expression of key components of the renin-angiotensin system during nephrogenesis. Article Snippet: Aim: This study investigates key components of the reninangiotensin system (RAS) which play a central role in nephrogenesis and possibly in fetal programming of arterial hypertension in adult life.. Methods: We compared a genetic rat model with inborn nephron deficit, the Munich Wistar Fromter rat (MWF), to normotensive Wistar rats during nephrogenesis at day 19 of fetal development (E19) and at postnatal day 7 (D7).. Results: At E19 renal mRNA of angiotensin II type 1a (AT1a) (–50%, P < 0.05) and type 1b (AT1b) (–55%, P < 0.05) receptors were significantly decreased and renal mRNA expression of angiotensin II type 2 (AT2) receptor was fivefold increased in MWF (n = 8) as compared to Wistar rats (n = 8). Gene Expression:Article Title: 2-arachidonoylglycerol signaling in forebrain regulates systemic energy metabolism Article Snippet: .. Primers and Quantitative RT-PCR:Article Title: Nipah virus Bangladesh infection elicits organ-specific innate and inflammatory responses in the marmoset model Article Snippet: RNA was extracted from tissues homogenized in TRIzol reagent or from blood and swabs mixed with TRIzol LS reagent using Direct-zol RNA Miniprep kits (Zymo Research, Irvine, CA). .. RT-qPCR assays were then run using QuantiFast RT-PCR mix (Qiagen, Hilden, Germany), probes targeting NiV M P gene (5′-ACATACAACTGGACCCARTGGTT-3′ and 5′-CACCCTCTCTCAGGGCTTGA-3′) (IDT, Coralville, IA), and Reverse Transcription Polymerase Chain Reaction:Article Title: Nipah virus Bangladesh infection elicits organ-specific innate and inflammatory responses in the marmoset model Article Snippet: RNA was extracted from tissues homogenized in TRIzol reagent or from blood and swabs mixed with TRIzol LS reagent using Direct-zol RNA Miniprep kits (Zymo Research, Irvine, CA). .. RT-qPCR assays were then run using QuantiFast RT-PCR mix (Qiagen, Hilden, Germany), probes targeting NiV M P gene (5′-ACATACAACTGGACCCARTGGTT-3′ and 5′-CACCCTCTCTCAGGGCTTGA-3′) (IDT, Coralville, IA), and |
