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fluorogenic hdac 1  (BPS Bioscience)


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    Structured Review

    BPS Bioscience fluorogenic hdac 1
    Fluorogenic Hdac 1, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorogenic+hdac+1/HDAC1+Fluorogenic+Assay+Kit/pm39357770-233-0-8
    Average 93 stars, based on 52 article reviews
    fluorogenic hdac 1 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: Design, synthesis and molecular modeling of new coumarin–thiazole derivatives as dual EGFR/HDAC1 inhibitors: in vitro and in vivo anticancer assays
    Article Snippet: MCF-7 (human breast adenocarcinoma), HCT116 (human colorectal carcinoma) and the normal human skin fibroblast (BJ-1) cell lines were purchased from the American Type Culture Collection (Rockville, MD, USA) and maintained in the RPMI-1640 medium, which was supplemented with 10% heat-inactivated FBS, 100 U per mL penicillin, and 100 U per mL streptomycin.

    Activity Assay:

    Article Title: 1,3,4-oxadiazole-tethered alkylidene acetohydrazides as potential anticancer agents: synthesis and in vitro biological evaluation
    Article Snippet: In this work, the synthesis and anticancer studies of 15 heterocyclic compounds (6a-6c, 7a-7c, 8a-8c, 9a-9c, and 10a-10c) bearing 1,3,4-oxadiazole-based alkylidene acetohydrazide moieties are reported.. The structural confirmation of these compounds was carried out using HRMS (Q-TOF), NMR, and single-crystal XRD (SC-XRD).. The cytotoxicity of the compounds was evaluated using cancer cell lines (4T1, MCF-7, PC3, and A549) and normal cell lines (HEK-293 & MCF-10A), with BG45, a selective HDAC3 inhibitor, as the reference compound.

    Article Title: Mitochondrial ATP production promotes T cell differentiation and function by regulating chromatin accessibility
    Article Snippet: After incubation for 1 h at 37 °C, the HDAC-Glo I/II reagent was added for 30 min at room temperature followed by luminescence acquisition using a Cytation 3 plate reader (Agilent). .. Cell-free recombinant HDAC activity assays (HDAC1 Fluorogenic Assay Kit and HDAC2 Fluorogenic Assay Kit; BPS Bioscience) were performed according to the manufacturer’s instructions. ..

    Article Title: A Hybrid compound H93 treats prostate cancer by directly binding UHRF1 and promoting protein dimerization
    Article Snippet: .. The inhibition effect of H93 on the deacetylase activity of class I HDACs was measured by the Fluorogenic HDAC1-6 Assay Kit (Cat#50061/50062/50073/50064/50065 /50076, BPS Bioscience, San Diego, CA, USA) according to the manufacturer’s instructions. .. Briefly, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme and different concentrations of H93, and the deacetylation produces a fluorophore that can then be measured using SpectraMax M5 multi-Mode Reader (Molecular Devices, San Jose, CA, USA).

    Histone Deacetylase Assay:

    Article Title: 1,3,4-oxadiazole-tethered alkylidene acetohydrazides as potential anticancer agents: synthesis and in vitro biological evaluation
    Article Snippet: In this work, the synthesis and anticancer studies of 15 heterocyclic compounds (6a-6c, 7a-7c, 8a-8c, 9a-9c, and 10a-10c) bearing 1,3,4-oxadiazole-based alkylidene acetohydrazide moieties are reported.. The structural confirmation of these compounds was carried out using HRMS (Q-TOF), NMR, and single-crystal XRD (SC-XRD).. The cytotoxicity of the compounds was evaluated using cancer cell lines (4T1, MCF-7, PC3, and A549) and normal cell lines (HEK-293 & MCF-10A), with BG45, a selective HDAC3 inhibitor, as the reference compound.

    Article Title: A Hybrid compound H93 treats prostate cancer by directly binding UHRF1 and promoting protein dimerization
    Article Snippet: .. The inhibition effect of H93 on the deacetylase activity of class I HDACs was measured by the Fluorogenic HDAC1-6 Assay Kit (Cat#50061/50062/50073/50064/50065 /50076, BPS Bioscience, San Diego, CA, USA) according to the manufacturer’s instructions. .. Briefly, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme and different concentrations of H93, and the deacetylation produces a fluorophore that can then be measured using SpectraMax M5 multi-Mode Reader (Molecular Devices, San Jose, CA, USA).

    Recombinant:

    Article Title: Mitochondrial ATP production promotes T cell differentiation and function by regulating chromatin accessibility
    Article Snippet: After incubation for 1 h at 37 °C, the HDAC-Glo I/II reagent was added for 30 min at room temperature followed by luminescence acquisition using a Cytation 3 plate reader (Agilent). .. Cell-free recombinant HDAC activity assays (HDAC1 Fluorogenic Assay Kit and HDAC2 Fluorogenic Assay Kit; BPS Bioscience) were performed according to the manufacturer’s instructions. ..

    Inhibition:

    Article Title: A Hybrid compound H93 treats prostate cancer by directly binding UHRF1 and promoting protein dimerization
    Article Snippet: .. The inhibition effect of H93 on the deacetylase activity of class I HDACs was measured by the Fluorogenic HDAC1-6 Assay Kit (Cat#50061/50062/50073/50064/50065 /50076, BPS Bioscience, San Diego, CA, USA) according to the manufacturer’s instructions. .. Briefly, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme and different concentrations of H93, and the deacetylation produces a fluorophore that can then be measured using SpectraMax M5 multi-Mode Reader (Molecular Devices, San Jose, CA, USA).

    Enzymatic Assay:

    Article Title: Design, synthesis, and biological evaluation of indazole-based histone deacetylase 6 inhibitors.
    Article Snippet: Histone deacetylases (HDACs) have been explored as anticancer targets for over two decades, with six HDAC inhibitors approved for clinical use.. However, these pan-HDAC inhibitors exhibit off-target effects, necessitating the development of isoform-selective inhibitors.. Among HDACs, HDAC6 has garnered attention due to its dual catalytic domains, cytoplasmic localization, and zinc-finger ubiquitin-binding domain (Zf-UBD).



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    In vitro inhibitory activity of compound 5 against histone deacetylase isotypes.

    Journal: Medicine

    Article Title: Design, synthesis, and biological evaluation of histone deacetylase inhibitor with novel salicylamide zinc binding group

    doi: 10.1097/MD.0000000000029049

    Figure Lengend Snippet: In vitro inhibitory activity of compound 5 against histone deacetylase isotypes.

    Article Snippet: HDAC fluorogenic assay kits (HDAC1, #50061; HDAC2, #50062; HDAC3, #50073; HDAC4, #50064; HDAC6, #50076; HDAC11, #50687) were bought from BPS Bioscience (San Diego, CA).

    Techniques: In Vitro, Activity Assay, Histone Deacetylase Assay

    Effect of compound 5 on the acetylation status of Histone H3 and α-tubulin. MDA-MB-231 cells were incubated with the indicated concentrations of compound 5 for 24 hours and the expression level of Histone H3, Ac-Histone H3, α-tubulin, Ac-α-tubulin, HDAC1, HDAC6, and GAPDH were analyzed using Western blotting. DMSO (D) was used as a negative control. GAPDH = glyceraldehyde 3-phosphate dehydrogenase, HDAC = Histone deacetylase.

    Journal: Medicine

    Article Title: Design, synthesis, and biological evaluation of histone deacetylase inhibitor with novel salicylamide zinc binding group

    doi: 10.1097/MD.0000000000029049

    Figure Lengend Snippet: Effect of compound 5 on the acetylation status of Histone H3 and α-tubulin. MDA-MB-231 cells were incubated with the indicated concentrations of compound 5 for 24 hours and the expression level of Histone H3, Ac-Histone H3, α-tubulin, Ac-α-tubulin, HDAC1, HDAC6, and GAPDH were analyzed using Western blotting. DMSO (D) was used as a negative control. GAPDH = glyceraldehyde 3-phosphate dehydrogenase, HDAC = Histone deacetylase.

    Article Snippet: HDAC fluorogenic assay kits (HDAC1, #50061; HDAC2, #50062; HDAC3, #50073; HDAC4, #50064; HDAC6, #50076; HDAC11, #50687) were bought from BPS Bioscience (San Diego, CA).

    Techniques: Incubation, Expressing, Western Blot, Negative Control, Histone Deacetylase Assay

    Molecular docking pose of compound 5 bound in the substrate-binding pocket of HDAC2 (PDB code: 4LXZ). The docking pose of compound 5 (lime) and the crystal structure of SAHA (pink) complex were overlapped in the substrate-binding pocket of HDAC2. The oxygen, nitrogen, and hydrogen atoms of compound 5 and SAHA are shown in red, blue, and white, respectively. The side chains of the binding site are colored according to the atom types (carbon, light blue; oxygen, red; nitrogen, blue) and labeled with their residue name. The hydrogen bonds are shown as dashed red lines. The docking poses are visualized using PyMOL1.3.

    Journal: Medicine

    Article Title: Design, synthesis, and biological evaluation of histone deacetylase inhibitor with novel salicylamide zinc binding group

    doi: 10.1097/MD.0000000000029049

    Figure Lengend Snippet: Molecular docking pose of compound 5 bound in the substrate-binding pocket of HDAC2 (PDB code: 4LXZ). The docking pose of compound 5 (lime) and the crystal structure of SAHA (pink) complex were overlapped in the substrate-binding pocket of HDAC2. The oxygen, nitrogen, and hydrogen atoms of compound 5 and SAHA are shown in red, blue, and white, respectively. The side chains of the binding site are colored according to the atom types (carbon, light blue; oxygen, red; nitrogen, blue) and labeled with their residue name. The hydrogen bonds are shown as dashed red lines. The docking poses are visualized using PyMOL1.3.

    Article Snippet: HDAC fluorogenic assay kits (HDAC1, #50061; HDAC2, #50062; HDAC3, #50073; HDAC4, #50064; HDAC6, #50076; HDAC11, #50687) were bought from BPS Bioscience (San Diego, CA).

    Techniques: Binding Assay, Labeling