adam10 (Santa Cruz Biotechnology)
Structured Review
Adam10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorogenic+adam10+substrate+peptide/ADAM10+siRNA/pmc04742152-159-4-9
Average 93 stars, based on 21 article reviews
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Transfection:Article Title: Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models Article Snippet: .. For the siRNA treatment, 10 to 20 μ l X-tremeGENE siRNA transfection reagent (Roche, Mannheim) diluted in 50 μ l RPMI was added to 120 pmol or 240 pmol BRCA1 siRNA (Cell Signaling) or Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site. Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown. Cell-Signaling:Article Title: Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models Article Snippet: .. For the siRNA treatment, 10 to 20 μ l X-tremeGENE siRNA transfection reagent (Roche, Mannheim) diluted in 50 μ l RPMI was added to 120 pmol or 240 pmol BRCA1 siRNA (Cell Signaling) or Incubator:Article Title: Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models Article Snippet: .. For the siRNA treatment, 10 to 20 μ l X-tremeGENE siRNA transfection reagent (Roche, Mannheim) diluted in 50 μ l RPMI was added to 120 pmol or 240 pmol BRCA1 siRNA (Cell Signaling) or Negative Control:Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site. Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown. Article Title: Proteolytic cleavage of amyloid precursor protein by ADAM10 promotes proliferation and migration via activating MAPKs pathway in tongue squamous cell carcinoma in vitro. Article Snippet: The antibodies used in this study were as follows: anti-APP N terminus (22C11) (Sigma, USA), anti-sAPPα (TECAN, Switzerland), anti-sAPPβ (Biolegend, USA), anti-ADAM10 (CST, USA), anti-p38 (CST, USA), antip-p38 (CST, USA), anti-ERK1/2 (CST, USA), anti-pERK1/2 (CST, USA), anti-GAPDH (CST, USA) and goat anti-rabbit/mouse IgG (CST, USA). .. The ERK1/2 agonist Pamoic acid disodium and p38 agonist 4-Hydroxylonchocarpin were purchased from MedChenExpress (MCE, USA) The human siRNAs including negative control siRNA (si-NC), APP siRNA (si-APP) and Article Title: GW501516-Mediated Targeting of Tetraspanin 15 Regulates ADAM10-Dependent N-Cadherin Cleavage in Invasive Bladder Cancer Cells. Article Snippet: .. Control siRNA (negative control for evaluating RNAi off-target effects, sc-37007), PPARβ/δ (sc-36305), Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target Plasmid Preparation:Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site. Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown. Control:Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site. Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown. Article Title: GW501516-Mediated Targeting of Tetraspanin 15 Regulates ADAM10-Dependent N-Cadherin Cleavage in Invasive Bladder Cancer Cells. Article Snippet: .. Control siRNA (negative control for evaluating RNAi off-target effects, sc-37007), PPARβ/δ (sc-36305), Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target Sequencing:Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target |
