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adam10  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology adam10
    Adam10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorogenic+adam10+substrate+peptide/ADAM10+siRNA/pmc04742152-159-4-9
    Average 93 stars, based on 21 article reviews
    adam10 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models
    Article Snippet: .. For the siRNA treatment, 10 to 20 μ l X-tremeGENE siRNA transfection reagent (Roche, Mannheim) diluted in 50 μ l RPMI was added to 120 pmol or 240 pmol BRCA1 siRNA (Cell Signaling) or ADAM10 siRNA (Santa Cruz), or not, in a final volume of 300 μ l and then was added to the cells for 2 h in a CO 2 incubator. .. 2.5 μ l siRNA with scrambled sequences in 100 μ l RPMI was used as control according to the manufacturer (Santa Cruz).

    Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site.
    Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown.

    Cell-Signaling:

    Article Title: Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models
    Article Snippet: .. For the siRNA treatment, 10 to 20 μ l X-tremeGENE siRNA transfection reagent (Roche, Mannheim) diluted in 50 μ l RPMI was added to 120 pmol or 240 pmol BRCA1 siRNA (Cell Signaling) or ADAM10 siRNA (Santa Cruz), or not, in a final volume of 300 μ l and then was added to the cells for 2 h in a CO 2 incubator. .. 2.5 μ l siRNA with scrambled sequences in 100 μ l RPMI was used as control according to the manufacturer (Santa Cruz).

    Incubator:

    Article Title: Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models
    Article Snippet: .. For the siRNA treatment, 10 to 20 μ l X-tremeGENE siRNA transfection reagent (Roche, Mannheim) diluted in 50 μ l RPMI was added to 120 pmol or 240 pmol BRCA1 siRNA (Cell Signaling) or ADAM10 siRNA (Santa Cruz), or not, in a final volume of 300 μ l and then was added to the cells for 2 h in a CO 2 incubator. .. 2.5 μ l siRNA with scrambled sequences in 100 μ l RPMI was used as control according to the manufacturer (Santa Cruz).

    Negative Control:

    Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site.
    Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown.

    Article Title: Proteolytic cleavage of amyloid precursor protein by ADAM10 promotes proliferation and migration via activating MAPKs pathway in tongue squamous cell carcinoma in vitro.
    Article Snippet: The antibodies used in this study were as follows: anti-APP N terminus (22C11) (Sigma, USA), anti-sAPPα (TECAN, Switzerland), anti-sAPPβ (Biolegend, USA), anti-ADAM10 (CST, USA), anti-p38 (CST, USA), antip-p38 (CST, USA), anti-ERK1/2 (CST, USA), anti-pERK1/2 (CST, USA), anti-GAPDH (CST, USA) and goat anti-rabbit/mouse IgG (CST, USA). .. The ERK1/2 agonist Pamoic acid disodium and p38 agonist 4-Hydroxylonchocarpin were purchased from MedChenExpress (MCE, USA) The human siRNAs including negative control siRNA (si-NC), APP siRNA (si-APP) and ADAM10 siRNA (si-ADAM10) were bought from Santa Cruz Biotechnology (USA). .. The human plasmids of pCAX and pCAXAPP751 (full length) and pCAX-sAPPα were purchased from Addgene (USA).

    Article Title: GW501516-Mediated Targeting of Tetraspanin 15 Regulates ADAM10-Dependent N-Cadherin Cleavage in Invasive Bladder Cancer Cells.
    Article Snippet: .. Control siRNA (negative control for evaluating RNAi off-target effects, sc-37007), PPARβ/δ (sc-36305), Adam10 (sc-41410), and Tspan15 (sc-90664) specific siRNA (pool of 3 target-specific 19–25 nucleotide siRNAs) were from Santa Cruz Biotechnology. ..

    Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc
    Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target ADAM10 mRNA sequence, while control siRNA (sc-37007, Santa Cruz, USA) was used as negative control. ..

    Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc
    Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target ADAM10 mRNA sequence, while control siRNA (sc-37007, Santa Cruz, USA) was used as negative control. ..

    Plasmid Preparation:

    Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site.
    Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown.

    Control:

    Article Title: Mapping and functional characterization of murine kidney injury molecule-1 proteolytic cleavage site.
    Article Snippet: Kidney injury molecule-1 (KIM-1), also known as T cell immunoglobulin and mucin domain 1 (TIM-1), is a transmembrane glycoprotein expressed on proximal tubule epithelia during acute kidney injury (AKI).. Extracellular domain of KIM-1 undergoes spontaneous and activated ectodomain shedding into urine and blood via metalloproteases.. Soluble KIM-1 (blood and urinary) is a reliable clinical biomarker of proximal tubular injury, but the biological significance of shedding remains unknown.

    Article Title: GW501516-Mediated Targeting of Tetraspanin 15 Regulates ADAM10-Dependent N-Cadherin Cleavage in Invasive Bladder Cancer Cells.
    Article Snippet: .. Control siRNA (negative control for evaluating RNAi off-target effects, sc-37007), PPARβ/δ (sc-36305), Adam10 (sc-41410), and Tspan15 (sc-90664) specific siRNA (pool of 3 target-specific 19–25 nucleotide siRNAs) were from Santa Cruz Biotechnology. ..

    Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc
    Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target ADAM10 mRNA sequence, while control siRNA (sc-37007, Santa Cruz, USA) was used as negative control. ..

    Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc
    Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target ADAM10 mRNA sequence, while control siRNA (sc-37007, Santa Cruz, USA) was used as negative control. ..

    Sequencing:

    Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc
    Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target ADAM10 mRNA sequence, while control siRNA (sc-37007, Santa Cruz, USA) was used as negative control. ..

    Article Title: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc
    Article Snippet: After an overnight incubation, the cells were transfected with different dilutions of siRNA using transfection Reagent (sc-29528, Santa Cruz, USA) as suggested by the manufacturer’s instructions. .. The small interference RNA (sc-41410, Santa Cruz, USA) was used to target ADAM10 mRNA sequence, while control siRNA (sc-37007, Santa Cruz, USA) was used as negative control. ..



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    a) Alexa Fluor 647-labeled AhlyH35A (7.5nM) was incubated with A549 cells in the presence of increasing concentrations of Peptide 88 or a control bicyclic peptide. Cell-associated fluorescence was quantified by flow cytometry and shown as histogram overlays. Negative control (cells only) shown in black; positive control (AhlyH35A without peptide) shown in red. b) Quantification of median fluorescence intensity plotted against peptide concentration. Data are normalized to the negative and positive controls. c) ADAM10 protease activation by Ahly (6µM) was measured using a whole-cell FRET peptide cleavage assay in the presence of Peptide 88 or a control bicyclic peptide (900µM). Mean of two biological replicates; error bars indicate standard deviation. Data were analysed using one-way ANOVA with Dunnett’s test: ns = not significant; ** = P < 0.01.

    Journal: bioRxiv

    Article Title: Discovery, characterisation and optimisation of bicyclic peptide inhibitors that disarm Staphylococcus aureus α-hemolysin

    doi: 10.64898/2026.03.09.710508

    Figure Lengend Snippet: a) Alexa Fluor 647-labeled AhlyH35A (7.5nM) was incubated with A549 cells in the presence of increasing concentrations of Peptide 88 or a control bicyclic peptide. Cell-associated fluorescence was quantified by flow cytometry and shown as histogram overlays. Negative control (cells only) shown in black; positive control (AhlyH35A without peptide) shown in red. b) Quantification of median fluorescence intensity plotted against peptide concentration. Data are normalized to the negative and positive controls. c) ADAM10 protease activation by Ahly (6µM) was measured using a whole-cell FRET peptide cleavage assay in the presence of Peptide 88 or a control bicyclic peptide (900µM). Mean of two biological replicates; error bars indicate standard deviation. Data were analysed using one-way ANOVA with Dunnett’s test: ns = not significant; ** = P < 0.01.

    Article Snippet: Following incubation, cells were washed once with 25mM Tris buffer, pH 8.0 and a fluorogenic ADAM10 substrate peptide (Mca-PLAQAV-Dpa-RSSSR-NH 2 ; R&D Systems) was added at a final concentration of 10μM.

    Techniques: Labeling, Incubation, Control, Fluorescence, Flow Cytometry, Negative Control, Positive Control, Concentration Assay, Activation Assay, Cleavage Assay, Standard Deviation