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f 7000 fluorescence spectrophotometer  (Hitachi Ltd)


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    Hitachi Ltd f 7000 fluorescence spectrophotometer
    F 7000 Fluorescence Spectrophotometer, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 99/100, based on 27216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence/F-7000/pm41880895-53-8-11
    Average 99 stars, based on 27216 article reviews
    f 7000 fluorescence spectrophotometer - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Exonuclease III-Proofread high-fidelity primer exchange reaction enables ultrasensitive analysis of prostate cancer MicroRNA.
    Article Snippet: The primer exchange reaction (PER) has emerged as a versatile isothermal amplification technique for biosensing, yet its practical application is severely hampered by non-specific hybridization-induced background signals.. Here, we report a high-fidelity PER (Hf-PER) strategy that overcomes this limitation through a rationally designed template architecture integrating exonuclease III (Exo III)-mediated target recycling, blocked primer binding, and self-priming amplification.. In this design, Exo III strictly discriminates perfectly matched targets from mismatched sequences, while the absence of free primers and magnetic bead separation effectively suppress spurious amplification.

    Fluorescence:

    Article Title: Exonuclease III-Proofread high-fidelity primer exchange reaction enables ultrasensitive analysis of prostate cancer MicroRNA.
    Article Snippet: The primer exchange reaction (PER) has emerged as a versatile isothermal amplification technique for biosensing, yet its practical application is severely hampered by non-specific hybridization-induced background signals.. Here, we report a high-fidelity PER (Hf-PER) strategy that overcomes this limitation through a rationally designed template architecture integrating exonuclease III (Exo III)-mediated target recycling, blocked primer binding, and self-priming amplification.. In this design, Exo III strictly discriminates perfectly matched targets from mismatched sequences, while the absence of free primers and magnetic bead separation effectively suppress spurious amplification.

    Article Title: An aggregation-induced emission fluorescent probe for selective lipid droplet imaging and real-time visualization of stress-related lipid metabolism
    Article Snippet: Absorption spectra were recorded on a SPECORD 210 PLUS spectrometer (Analytikjena, Germany). .. Fluorescence spectra were recorded on an F-7000 fluorescence spectrophotometer (Hitachi, Japan) with an excitation voltage of 700 V. For spectral measurements, a 3.0 mL test solution was prepared by mixing 0.03 mL of the probe stock solution (1.0 mM) with 2.97 mL of the indicated solvent. ..

    Article Title: A simple self-mediated nucleic acid elongation-driven amplification strategy for sensitive detection of terminal deoxynucleotidyl transferase activity
    Article Snippet: Terminal deoxynucleotidyl transferase (TdT) plays a pivotal role in the human immune system.. Its increased activity in leukemic cells renders it a vital biomarker for leukemia.. However, current TdT assays usually involve elaborate protocols and high costs.

    Article Title: Non-sewered sanitation systems reshape microplastics into chemically complex and reactive contaminants
    Article Snippet: Dissolved organic carbon (DOC) was analyzed using a total organic carbon analyzer (TOC-LCPH/CPN, Shimadzu). .. The pretreated samples (i.e., following the centrifugation and filtration described above) were diluted with 0.1mol/L phosphate buffer (pH = 7.0) to achieve afinalDOCcontent of about 10mg/L and then the fluorescence excitation-emission matrix (EEM) was measured at room temperature (25 °C) with an F-7000 fluorescence spectrophotometer (Hitachi, Japan). ..

    Article Title: Ferrocene anchored fluorescent cellulose nanofibers for ultrasensitive detection, adsorption, and Fenton degradation of norfloxacin: VOSviewer analysis, performance, mechanism, & RSM optimization.
    Article Snippet: As revealed by in-depth state-of-the-art literature analysis using VOSviewer, no study to date has been specifically reported on the Ferrocene carboxaldehyde anchored on the aminated cellulose nanofibers (FcCA@ACNFs) multifunctional hybrid fluorescent sensor, especially for comprehensive wastewater remediation encompassing trace-level detection, efficient adsorption, and Fenton-based degradation of Norfloxacin (NOR) antibiotic from wastewater.. Hence, this study has addressed this aforementioned shortcoming by critically synthesizing and comprehensively characterizing a novel fluorescent sensor, FcCA@ACNFs, formed via covalent Schiff base bonding between the Ferrocene carboxaldehyde (FcCA) and aminated cellulose nanofibers (ACNFs).. The fluorescence was significantly enhanced in the presence of NOR, exhibiting a high turn-on selectivity with LOD of 0.97 nM in the linear range of 1-100 mg L. The average fluorescence lifetime, ⟨τ⟩, estimated using time-resolved fluorescence lifetime spectroscopy, was 3.4905 ns for a higher concentration of NOR in the FcCA@ACNFsNOR complex, indicating a near-high analyte concentration.

    Article Title: Effects of chlorogenic acid on the interfacial properties and physical stability of sunflower oil body emulsions: Insights from a reconstructed protein-emulsion model
    Article Snippet: .. Fluorescence spectra were recorded on a spectrofluorometer (F-7000, Hitachi, Japan) at an excitation wavelength of 280 nm, and emission was monitored over the range of 290 to 500 nm with slit widths set to 5 nm ( ). ..

    Spectrophotometry:

    Article Title: Exonuclease III-Proofread high-fidelity primer exchange reaction enables ultrasensitive analysis of prostate cancer MicroRNA.
    Article Snippet: The primer exchange reaction (PER) has emerged as a versatile isothermal amplification technique for biosensing, yet its practical application is severely hampered by non-specific hybridization-induced background signals.. Here, we report a high-fidelity PER (Hf-PER) strategy that overcomes this limitation through a rationally designed template architecture integrating exonuclease III (Exo III)-mediated target recycling, blocked primer binding, and self-priming amplification.. In this design, Exo III strictly discriminates perfectly matched targets from mismatched sequences, while the absence of free primers and magnetic bead separation effectively suppress spurious amplification.

    Article Title: An aggregation-induced emission fluorescent probe for selective lipid droplet imaging and real-time visualization of stress-related lipid metabolism
    Article Snippet: Absorption spectra were recorded on a SPECORD 210 PLUS spectrometer (Analytikjena, Germany). .. Fluorescence spectra were recorded on an F-7000 fluorescence spectrophotometer (Hitachi, Japan) with an excitation voltage of 700 V. For spectral measurements, a 3.0 mL test solution was prepared by mixing 0.03 mL of the probe stock solution (1.0 mM) with 2.97 mL of the indicated solvent. ..

    Article Title: A simple self-mediated nucleic acid elongation-driven amplification strategy for sensitive detection of terminal deoxynucleotidyl transferase activity
    Article Snippet: Terminal deoxynucleotidyl transferase (TdT) plays a pivotal role in the human immune system.. Its increased activity in leukemic cells renders it a vital biomarker for leukemia.. However, current TdT assays usually involve elaborate protocols and high costs.

    Article Title: Non-sewered sanitation systems reshape microplastics into chemically complex and reactive contaminants
    Article Snippet: Dissolved organic carbon (DOC) was analyzed using a total organic carbon analyzer (TOC-LCPH/CPN, Shimadzu). .. The pretreated samples (i.e., following the centrifugation and filtration described above) were diluted with 0.1mol/L phosphate buffer (pH = 7.0) to achieve afinalDOCcontent of about 10mg/L and then the fluorescence excitation-emission matrix (EEM) was measured at room temperature (25 °C) with an F-7000 fluorescence spectrophotometer (Hitachi, Japan). ..

    Article Title: Ferrocene anchored fluorescent cellulose nanofibers for ultrasensitive detection, adsorption, and Fenton degradation of norfloxacin: VOSviewer analysis, performance, mechanism, & RSM optimization.
    Article Snippet: As revealed by in-depth state-of-the-art literature analysis using VOSviewer, no study to date has been specifically reported on the Ferrocene carboxaldehyde anchored on the aminated cellulose nanofibers (FcCA@ACNFs) multifunctional hybrid fluorescent sensor, especially for comprehensive wastewater remediation encompassing trace-level detection, efficient adsorption, and Fenton-based degradation of Norfloxacin (NOR) antibiotic from wastewater.. Hence, this study has addressed this aforementioned shortcoming by critically synthesizing and comprehensively characterizing a novel fluorescent sensor, FcCA@ACNFs, formed via covalent Schiff base bonding between the Ferrocene carboxaldehyde (FcCA) and aminated cellulose nanofibers (ACNFs).. The fluorescence was significantly enhanced in the presence of NOR, exhibiting a high turn-on selectivity with LOD of 0.97 nM in the linear range of 1-100 mg L. The average fluorescence lifetime, ⟨τ⟩, estimated using time-resolved fluorescence lifetime spectroscopy, was 3.4905 ns for a higher concentration of NOR in the FcCA@ACNFsNOR complex, indicating a near-high analyte concentration.

    Solvent:

    Article Title: An aggregation-induced emission fluorescent probe for selective lipid droplet imaging and real-time visualization of stress-related lipid metabolism
    Article Snippet: Absorption spectra were recorded on a SPECORD 210 PLUS spectrometer (Analytikjena, Germany). .. Fluorescence spectra were recorded on an F-7000 fluorescence spectrophotometer (Hitachi, Japan) with an excitation voltage of 700 V. For spectral measurements, a 3.0 mL test solution was prepared by mixing 0.03 mL of the probe stock solution (1.0 mM) with 2.97 mL of the indicated solvent. ..

    Analysis:

    Article Title: A simple self-mediated nucleic acid elongation-driven amplification strategy for sensitive detection of terminal deoxynucleotidyl transferase activity
    Article Snippet: Terminal deoxynucleotidyl transferase (TdT) plays a pivotal role in the human immune system.. Its increased activity in leukemic cells renders it a vital biomarker for leukemia.. However, current TdT assays usually involve elaborate protocols and high costs.

    Activity Assay:

    Article Title: A simple self-mediated nucleic acid elongation-driven amplification strategy for sensitive detection of terminal deoxynucleotidyl transferase activity
    Article Snippet: Terminal deoxynucleotidyl transferase (TdT) plays a pivotal role in the human immune system.. Its increased activity in leukemic cells renders it a vital biomarker for leukemia.. However, current TdT assays usually involve elaborate protocols and high costs.

    Centrifugation:

    Article Title: Non-sewered sanitation systems reshape microplastics into chemically complex and reactive contaminants
    Article Snippet: Dissolved organic carbon (DOC) was analyzed using a total organic carbon analyzer (TOC-LCPH/CPN, Shimadzu). .. The pretreated samples (i.e., following the centrifugation and filtration described above) were diluted with 0.1mol/L phosphate buffer (pH = 7.0) to achieve afinalDOCcontent of about 10mg/L and then the fluorescence excitation-emission matrix (EEM) was measured at room temperature (25 °C) with an F-7000 fluorescence spectrophotometer (Hitachi, Japan). ..

    Filtration:

    Article Title: Non-sewered sanitation systems reshape microplastics into chemically complex and reactive contaminants
    Article Snippet: Dissolved organic carbon (DOC) was analyzed using a total organic carbon analyzer (TOC-LCPH/CPN, Shimadzu). .. The pretreated samples (i.e., following the centrifugation and filtration described above) were diluted with 0.1mol/L phosphate buffer (pH = 7.0) to achieve afinalDOCcontent of about 10mg/L and then the fluorescence excitation-emission matrix (EEM) was measured at room temperature (25 °C) with an F-7000 fluorescence spectrophotometer (Hitachi, Japan). ..



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