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macrophage gpat3 visualization employed tsa plus fluorescent tricolor staining kit  (Servicebio Inc)

 
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    Structured Review

    Servicebio Inc macrophage gpat3 visualization employed tsa plus fluorescent tricolor staining kit
    Macrophage Gpat3 Visualization Employed Tsa Plus Fluorescent Tricolor Staining Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+visualization/kit+tsa/pm40769290-153-0-11
    Average 86 stars, based on 1 article reviews
    macrophage gpat3 visualization employed tsa plus fluorescent tricolor staining kit - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Multiplex Assay:

    Article Title: A microbiota-tryptophol-AhR axis mediates the gut-kidney protective effects of Hushen Tongfengtai Granules in hyperuricemic nephropathy.
    Article Snippet: Background: Hyperuricemia (HUA) may result in hyperuricemic nephropathy (HN), and gut dysbiosis with barrier dysfunction can worsen disease progression.. Hushen Tongfengtai granules (HSTFT), a traditional Chinese herbal prescription, have been used clinically to mitigate HUA and related renal injury.. However, the mechanisms behind their effects remain to be explored.

    Article Title: Materials-Guided Gene-Ionizable Lipid Nanoparticles to Reverse Iron-Associated Immune Resistance in Renal Cancer.
    Article Snippet: .. For animal tissues, multiplex fluorescence staining was performed using a TSA multiplex kit (Servicebio, China). ..

    Immunofluorescence:

    Article Title: A microbiota-tryptophol-AhR axis mediates the gut-kidney protective effects of Hushen Tongfengtai Granules in hyperuricemic nephropathy.
    Article Snippet: Background: Hyperuricemia (HUA) may result in hyperuricemic nephropathy (HN), and gut dysbiosis with barrier dysfunction can worsen disease progression.. Hushen Tongfengtai granules (HSTFT), a traditional Chinese herbal prescription, have been used clinically to mitigate HUA and related renal injury.. However, the mechanisms behind their effects remain to be explored.

    Article Title: Targeting the c-Jun–Irf8–CD36 Axis Attenuates Fibrotic Scar Formation and Promotes Functional Recovery after Spinal Cord Injury
    Article Snippet: Slides were treated D ow nloaded from https://academ ic.oup.com /burnstraum a/advance-article/doi/10.1093/burnst/tkag020/8516470 by guest on 01 M ay 2026 UN CO RR EC TE D MA NU SC RI PT with mounting medium containing DAPI (Beyotime Biotechnology) and imaged using an upright fluorescence microscope (Leica). .. For multiple immunofluorescence staining, TSA (tyramide signal amplification) fluorescent dye kits and digital scanners for tissue sections were purchased from Servicebio Co., Ltd (Wuhan, China). ..

    Staining:

    Article Title: A microbiota-tryptophol-AhR axis mediates the gut-kidney protective effects of Hushen Tongfengtai Granules in hyperuricemic nephropathy.
    Article Snippet: Background: Hyperuricemia (HUA) may result in hyperuricemic nephropathy (HN), and gut dysbiosis with barrier dysfunction can worsen disease progression.. Hushen Tongfengtai granules (HSTFT), a traditional Chinese herbal prescription, have been used clinically to mitigate HUA and related renal injury.. However, the mechanisms behind their effects remain to be explored.

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions
    Article Snippet: Fluorescence images were captured using the Nikon or Zeiss inverted fluorescence microscope. .. The dewaxed sections underwent antigen retrieval and were subsequently stained following the instructions of the TSA staining kit (Servicebio Technology Co., Ltd., Wuhan, China, cat# G1259). ..

    Article Title: Targeting the c-Jun–Irf8–CD36 Axis Attenuates Fibrotic Scar Formation and Promotes Functional Recovery after Spinal Cord Injury
    Article Snippet: Slides were treated D ow nloaded from https://academ ic.oup.com /burnstraum a/advance-article/doi/10.1093/burnst/tkag020/8516470 by guest on 01 M ay 2026 UN CO RR EC TE D MA NU SC RI PT with mounting medium containing DAPI (Beyotime Biotechnology) and imaged using an upright fluorescence microscope (Leica). .. For multiple immunofluorescence staining, TSA (tyramide signal amplification) fluorescent dye kits and digital scanners for tissue sections were purchased from Servicebio Co., Ltd (Wuhan, China). ..

    Article Title: p300-mediated histone H3K18 lactylation promotes mitochondrial ROS accumulation via mitophagy inhibition to potentiate dopamine agonists efficacy in prolactinomas
    Article Snippet: .. For TSA staining (used when primary antibodies are from the same species), a TSA Plus kit (Servicebio) was used according to the manufacturer's instructions. ..

    Article Title: Graphdiyne-Ivy fiber neural scaffold promotes stem cell directed differentiation and neuronal maturation
    Article Snippet: Optical density (OD) values were measured using a microplate reader (Thermo Varioskan LUX, Singapore) to quantify cell viability. .. Cytotoxicity was assessed via Live/Dead double fluorescence staining (Servicebio, China), with live cells stained green and dead cells stained red. .. The stained samples were observed under a fluorescence microscope (Leica DMI8, Germany) to qualitatively assess cell viability and distribution on the scaffolds.

    Article Title: Materials-Guided Gene-Ionizable Lipid Nanoparticles to Reverse Iron-Associated Immune Resistance in Renal Cancer.
    Article Snippet: .. For animal tissues, multiplex fluorescence staining was performed using a TSA multiplex kit (Servicebio, China). ..

    Article Title: Phenolic fraction of Elsholtzia penduliflora W.W.Sm. ameliorates influenza A virus-induced acute lung injury by inhibiting the IDO-1-mitochondria-STAT1 signaling axis.
    Article Snippet: Ethnopharmacological relevance: Severe influenza is driven by immunometabolic dysregulation, highlighting the need for novel therapeutic strategies.. Elsholtzia penduliflora W.W.Sm. (E. penduliflora) is a traditional ethnomedicine that has long utilized by Miao communities in southwestern China for the treatment of respiratory infections, including influenza and pneumonia.. Although its antiviral potential is increasingly recognized, the specific active constituents and the precise immunometabolic mechanisms through which E. penduliflora alleviates virus-induced lung injury remain poorly understood.

    Fluorescence:

    Article Title: A microbiota-tryptophol-AhR axis mediates the gut-kidney protective effects of Hushen Tongfengtai Granules in hyperuricemic nephropathy.
    Article Snippet: Background: Hyperuricemia (HUA) may result in hyperuricemic nephropathy (HN), and gut dysbiosis with barrier dysfunction can worsen disease progression.. Hushen Tongfengtai granules (HSTFT), a traditional Chinese herbal prescription, have been used clinically to mitigate HUA and related renal injury.. However, the mechanisms behind their effects remain to be explored.

    Article Title: Graphdiyne-Ivy fiber neural scaffold promotes stem cell directed differentiation and neuronal maturation
    Article Snippet: Optical density (OD) values were measured using a microplate reader (Thermo Varioskan LUX, Singapore) to quantify cell viability. .. Cytotoxicity was assessed via Live/Dead double fluorescence staining (Servicebio, China), with live cells stained green and dead cells stained red. .. The stained samples were observed under a fluorescence microscope (Leica DMI8, Germany) to qualitatively assess cell viability and distribution on the scaffolds.

    Article Title: Materials-Guided Gene-Ionizable Lipid Nanoparticles to Reverse Iron-Associated Immune Resistance in Renal Cancer.
    Article Snippet: .. For animal tissues, multiplex fluorescence staining was performed using a TSA multiplex kit (Servicebio, China). ..

    other:

    Article Title: Persistent Wnt/β-catenin signaling disables soft palatogenesis and palatal osteogenesis by inducing mesenchymal condensation
    Article Snippet: The primary antibodies against Lef1 (ab137827, Abcam, Cambridge, MA, United States), integrin ɑv (bs-1310R, Bioss Antibodies Inc., Beijing, China), ColII (K0921, Santa Cruz), Foxf1 (ab308633, Abcam), Dlx5 (10592-1-AP, Proteintech Group Inc., Wuhan, China), Runx2 (sc-101145, Santa Cruz Biotechnology Co., Ltd., Shanghai, China), Sox9 (ab185966, Abcam), Osterix (ab209484, Abcam), phospho-Smad1/5/8 (13820S, Cell Signaling Technology, Danvers, MA, United States), ɑ-SMA (ab7817, Abcam), ColI ( P28372 -BIF, Abmart, Shanghai, China), p-ERK1/2 (4370T, Cell Signaling Technology), Etv4 (10684-1-AP, Cloud-Clone Corp., Wuhan, China), Tbx15 (YN1399, Immunoway, Thermo Fisher Scientific, Shanghai, China), and Scx (ab307722, Abcam) were applied for immunofluorescence.

    Amplification:

    Article Title: Targeting the c-Jun–Irf8–CD36 Axis Attenuates Fibrotic Scar Formation and Promotes Functional Recovery after Spinal Cord Injury
    Article Snippet: Slides were treated D ow nloaded from https://academ ic.oup.com /burnstraum a/advance-article/doi/10.1093/burnst/tkag020/8516470 by guest on 01 M ay 2026 UN CO RR EC TE D MA NU SC RI PT with mounting medium containing DAPI (Beyotime Biotechnology) and imaged using an upright fluorescence microscope (Leica). .. For multiple immunofluorescence staining, TSA (tyramide signal amplification) fluorescent dye kits and digital scanners for tissue sections were purchased from Servicebio Co., Ltd (Wuhan, China). ..

    Article Title: Phenolic fraction of Elsholtzia penduliflora W.W.Sm. ameliorates influenza A virus-induced acute lung injury by inhibiting the IDO-1-mitochondria-STAT1 signaling axis.
    Article Snippet: Ethnopharmacological relevance: Severe influenza is driven by immunometabolic dysregulation, highlighting the need for novel therapeutic strategies.. Elsholtzia penduliflora W.W.Sm. (E. penduliflora) is a traditional ethnomedicine that has long utilized by Miao communities in southwestern China for the treatment of respiratory infections, including influenza and pneumonia.. Although its antiviral potential is increasingly recognized, the specific active constituents and the precise immunometabolic mechanisms through which E. penduliflora alleviates virus-induced lung injury remain poorly understood.



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    Image Search Results


    Parameters of different jet injection protocols.

    Journal: Vaccines

    Article Title: Jet Injection of Naked mRNA Encoding the RBD of the SARS-CoV-2 Spike Protein Induces a High Level of a Specific Immune Response in Mice

    doi: 10.3390/vaccines13010065

    Figure Lengend Snippet: Parameters of different jet injection protocols.

    Article Snippet: Fluorescence activity was assessed qualitatively one-hour post-injection using an in vivo fluorescence and bioluminescence visualization and analysis system (VISQUE InVivo Series Smart-LF, Vieworks Co., Ltd., Anyang-si, Republic of Korea).

    Techniques: Injection, Fluorescence

    ( A ). Fluorescence microphotographs of muscle tissue obtained 3 days after immunization by jet injection of GFP plasmid. Samples were collected and then cut into 1 mm thick slices to visualize GFP expression: ( A’ ) control mice (without jet injection); ( B’ ) protocol No. 1; ( C’ ) protocol No. 2; ( D’ ) protocol No. 3. ( B ). Fluorescence intensity was plotted using computer-generated signal processing. (A) control mice (without jet injection); (B) protocol No. 1; (C) protocol No. 2; (D) protocol No. 3. Statistical significance was determined by nonparametric Kruskal–Wallis analysis with correction for multiple comparisons and Dunn’s test for statistical hypotheses (** p < 0.01, **** p < 0.0001).

    Journal: Vaccines

    Article Title: Jet Injection of Naked mRNA Encoding the RBD of the SARS-CoV-2 Spike Protein Induces a High Level of a Specific Immune Response in Mice

    doi: 10.3390/vaccines13010065

    Figure Lengend Snippet: ( A ). Fluorescence microphotographs of muscle tissue obtained 3 days after immunization by jet injection of GFP plasmid. Samples were collected and then cut into 1 mm thick slices to visualize GFP expression: ( A’ ) control mice (without jet injection); ( B’ ) protocol No. 1; ( C’ ) protocol No. 2; ( D’ ) protocol No. 3. ( B ). Fluorescence intensity was plotted using computer-generated signal processing. (A) control mice (without jet injection); (B) protocol No. 1; (C) protocol No. 2; (D) protocol No. 3. Statistical significance was determined by nonparametric Kruskal–Wallis analysis with correction for multiple comparisons and Dunn’s test for statistical hypotheses (** p < 0.01, **** p < 0.0001).

    Article Snippet: Fluorescence activity was assessed qualitatively one-hour post-injection using an in vivo fluorescence and bioluminescence visualization and analysis system (VISQUE InVivo Series Smart-LF, Vieworks Co., Ltd., Anyang-si, Republic of Korea).

    Techniques: Fluorescence, Injection, Plasmid Preparation, Expressing, Control, Generated