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Becton Dickinson facs-diva
Facs Diva, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+data+analyses/facs+diva/pmc07868409-132-8-7
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facs-diva - by Bioz Stars, 2026-10
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Related Articles

Suspension:

Article Title: Effects of SIPA1L1 on trabecular meshwork extracellular matrix protein accumulation and cellular phagocytosis in POAG.
Article Snippet: .. The cell suspension was 570 then collected, and the fluorescence intensity of the pHrodoTM bioparticles was 571 analyzed using the BD FACS DIVA System (BD Fotessa). ..

Fluorescence:

Article Title: Effects of SIPA1L1 on trabecular meshwork extracellular matrix protein accumulation and cellular phagocytosis in POAG.
Article Snippet: .. The cell suspension was 570 then collected, and the fluorescence intensity of the pHrodoTM bioparticles was 571 analyzed using the BD FACS DIVA System (BD Fotessa). ..

FACS:

Article Title: Effects of SIPA1L1 on trabecular meshwork extracellular matrix protein accumulation and cellular phagocytosis in POAG.
Article Snippet: .. The cell suspension was 570 then collected, and the fluorescence intensity of the pHrodoTM bioparticles was 571 analyzed using the BD FACS DIVA System (BD Fotessa). ..

Article Title: A Unified Atlas of T cell Glycophysiology
Article Snippet: .. Flow cytometry analysis was performed on Cytek Aurora (3 lasers, violet (405 nm), blue (488 nm), and red (640 nm)) and BD FACS Diva. .. Single color controls for antibodies were prepared using UltraComp eBeads (Invitrogen).

Article Title: IRE1α-XBP1 safeguards hematopoietic stem and progenitor cells by restricting pro-leukemogenic gene programs.
Article Snippet: Hematopoietic stem cells must mitigate myriad stressors throughout their lifetime to ensure normal blood cell generation.. Here, we uncover unfolded protein response stress sensor inositol-requiring enzyme-1α (IRE1α) signaling in hematopoietic stem and progenitor cells (HSPCs) as a safeguard against myeloid leukemogenesis.. Activated in part by an NADPH oxidase-2 mechanism, IRE1α-induced X-box binding protein-1 (XBP1) mediated repression of pro-leukemogenic programs exemplified by the Wnt–β-catenin pathway.

Article Title: Interplay between host humoral pattern recognition molecules controls undue immune responses against Aspergillus fumigatus
Article Snippet: Dead cells were excluded with the fixable viability dye eFluor 506 staining-kit (Life Technologies). .. The samples were acquired using LSR II (BD Biosciences), and the data were analyzed by BD FACS DIVA (BD Biosciences) and FlowJo. ..

Article Title: 3-Bromopyruvate boosts the effect of chemotherapy in acute myeloid leukemia by a pro-oxidant mechanism.
Article Snippet: Acute myeloid leukemia (AML) comprises a diverse group of blood cancers with varying genetic, phenotypic, and clinical traits, making development of targeted therapy challenging.. Metabolic reprogramming in AML has been described as relevant for chemotherapy effectiveness.. 3-Bromopyruvate (3-BP) is an anticancer agent that undermines energy metabolism of cancer cells.

Article Title: Mir221/222 drive synovial hyperplasia and arthritis by targeting cell cycle inhibitors and chromatin remodeling components
Article Snippet: Zombie Green (Sigma 423112) or NIR (Sigma 77184) and DAPI (Invitrogen D1306) were used for live/dead exclusion. .. Analysis was performed using a FACS Canto II Flow cytometer (BD Biosciences) and FACS Diva (BD Biosciences) or FlowJo software (FlowJo, LLC), and cell sorting was performed using a FACS Aria III Cell Sorter (BD Biosciences). .. Samples were collected in RIPA buffer (1% NP-40, 0.1% sodium dodecyl sulfate [SDS], 150 mM NaCl, 50 mM Tris–HCl, pH 7.4, 1 mM EDTA supplemented with protease [Roche] and phosphatase inhibitors [Sigma-Aldrich]), separated by SDS-PAGE (8 and 12.5%), transferred to nitrocellulose membranes (Millipore), and probed overnight with the following Abs: ACTIN (sc-1615, Santa Cruz Biotechnology, 1:2000), CDKN1B (3688, Cell Signaling, 1:1000), CDKN1C (ab-4058, Abcam, 1/800), and SMARCA1 (ab-172483, Abcam, 1/1000).

Article Title: Temporal Immune Profiling in the CSF and Blood of Patients with Aneurysmal Subarachnoid Hemorrhage
Article Snippet: .. Sample acquisition was conducted using a FACSymphony (BD Biosciences) flow cytometer and BD FACS DIVA software. .. All gating and event analyses were performed in FlowJo v10 (BD biosciences).

Flow Cytometry:

Article Title: A Unified Atlas of T cell Glycophysiology
Article Snippet: .. Flow cytometry analysis was performed on Cytek Aurora (3 lasers, violet (405 nm), blue (488 nm), and red (640 nm)) and BD FACS Diva. .. Single color controls for antibodies were prepared using UltraComp eBeads (Invitrogen).

Article Title: IRE1α-XBP1 safeguards hematopoietic stem and progenitor cells by restricting pro-leukemogenic gene programs.
Article Snippet: Hematopoietic stem cells must mitigate myriad stressors throughout their lifetime to ensure normal blood cell generation.. Here, we uncover unfolded protein response stress sensor inositol-requiring enzyme-1α (IRE1α) signaling in hematopoietic stem and progenitor cells (HSPCs) as a safeguard against myeloid leukemogenesis.. Activated in part by an NADPH oxidase-2 mechanism, IRE1α-induced X-box binding protein-1 (XBP1) mediated repression of pro-leukemogenic programs exemplified by the Wnt–β-catenin pathway.

Article Title: 3-Bromopyruvate boosts the effect of chemotherapy in acute myeloid leukemia by a pro-oxidant mechanism.
Article Snippet: Acute myeloid leukemia (AML) comprises a diverse group of blood cancers with varying genetic, phenotypic, and clinical traits, making development of targeted therapy challenging.. Metabolic reprogramming in AML has been described as relevant for chemotherapy effectiveness.. 3-Bromopyruvate (3-BP) is an anticancer agent that undermines energy metabolism of cancer cells.

Article Title: Mir221/222 drive synovial hyperplasia and arthritis by targeting cell cycle inhibitors and chromatin remodeling components
Article Snippet: Zombie Green (Sigma 423112) or NIR (Sigma 77184) and DAPI (Invitrogen D1306) were used for live/dead exclusion. .. Analysis was performed using a FACS Canto II Flow cytometer (BD Biosciences) and FACS Diva (BD Biosciences) or FlowJo software (FlowJo, LLC), and cell sorting was performed using a FACS Aria III Cell Sorter (BD Biosciences). .. Samples were collected in RIPA buffer (1% NP-40, 0.1% sodium dodecyl sulfate [SDS], 150 mM NaCl, 50 mM Tris–HCl, pH 7.4, 1 mM EDTA supplemented with protease [Roche] and phosphatase inhibitors [Sigma-Aldrich]), separated by SDS-PAGE (8 and 12.5%), transferred to nitrocellulose membranes (Millipore), and probed overnight with the following Abs: ACTIN (sc-1615, Santa Cruz Biotechnology, 1:2000), CDKN1B (3688, Cell Signaling, 1:1000), CDKN1C (ab-4058, Abcam, 1/800), and SMARCA1 (ab-172483, Abcam, 1/1000).

Article Title: Temporal Immune Profiling in the CSF and Blood of Patients with Aneurysmal Subarachnoid Hemorrhage
Article Snippet: .. Sample acquisition was conducted using a FACSymphony (BD Biosciences) flow cytometer and BD FACS DIVA software. .. All gating and event analyses were performed in FlowJo v10 (BD biosciences).

other:

Article Title: Stanniocalcin-1 promotes temozolomide resistance of glioblastoma through regulation of MGMT
Article Snippet: The rate of apoptosis in each sample was analyzed using a flow cytometer (BD FACSCanto II) and calculated using the BD FACS-Diva software.

Software:

Article Title: IRE1α-XBP1 safeguards hematopoietic stem and progenitor cells by restricting pro-leukemogenic gene programs.
Article Snippet: Hematopoietic stem cells must mitigate myriad stressors throughout their lifetime to ensure normal blood cell generation.. Here, we uncover unfolded protein response stress sensor inositol-requiring enzyme-1α (IRE1α) signaling in hematopoietic stem and progenitor cells (HSPCs) as a safeguard against myeloid leukemogenesis.. Activated in part by an NADPH oxidase-2 mechanism, IRE1α-induced X-box binding protein-1 (XBP1) mediated repression of pro-leukemogenic programs exemplified by the Wnt–β-catenin pathway.

Article Title: 3-Bromopyruvate boosts the effect of chemotherapy in acute myeloid leukemia by a pro-oxidant mechanism.
Article Snippet: Acute myeloid leukemia (AML) comprises a diverse group of blood cancers with varying genetic, phenotypic, and clinical traits, making development of targeted therapy challenging.. Metabolic reprogramming in AML has been described as relevant for chemotherapy effectiveness.. 3-Bromopyruvate (3-BP) is an anticancer agent that undermines energy metabolism of cancer cells.

Article Title: Mir221/222 drive synovial hyperplasia and arthritis by targeting cell cycle inhibitors and chromatin remodeling components
Article Snippet: Zombie Green (Sigma 423112) or NIR (Sigma 77184) and DAPI (Invitrogen D1306) were used for live/dead exclusion. .. Analysis was performed using a FACS Canto II Flow cytometer (BD Biosciences) and FACS Diva (BD Biosciences) or FlowJo software (FlowJo, LLC), and cell sorting was performed using a FACS Aria III Cell Sorter (BD Biosciences). .. Samples were collected in RIPA buffer (1% NP-40, 0.1% sodium dodecyl sulfate [SDS], 150 mM NaCl, 50 mM Tris–HCl, pH 7.4, 1 mM EDTA supplemented with protease [Roche] and phosphatase inhibitors [Sigma-Aldrich]), separated by SDS-PAGE (8 and 12.5%), transferred to nitrocellulose membranes (Millipore), and probed overnight with the following Abs: ACTIN (sc-1615, Santa Cruz Biotechnology, 1:2000), CDKN1B (3688, Cell Signaling, 1:1000), CDKN1C (ab-4058, Abcam, 1/800), and SMARCA1 (ab-172483, Abcam, 1/1000).

Article Title: Temporal Immune Profiling in the CSF and Blood of Patients with Aneurysmal Subarachnoid Hemorrhage
Article Snippet: .. Sample acquisition was conducted using a FACSymphony (BD Biosciences) flow cytometer and BD FACS DIVA software. .. All gating and event analyses were performed in FlowJo v10 (BD biosciences).



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Image Search Results


Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

Journal: bioRxiv

Article Title: A 3-genes interferon signature predicts sustained complete remission in pediatric AML patients

doi: 10.1101/2025.07.17.664572

Figure Lengend Snippet: Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

Article Snippet: Statistical analyses of the flow cytometry data were performed using GraphPad Prism (v. 10.3.1).

Techniques: Flow Cytometry, Biomarker Discovery, Microscopy, Expressing