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Becton Dickinson bd lsr ii
Bd Lsr Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

Labeling:

Article Title: Reduced function of the adaptor SH2B3 promotes T1D via altered cytokine-regulated, T cell intrinsic immune tolerance.
Article Snippet: Genome-wide association studies have identified SH2B3 as an important non-MHC gene for islet autoimmunity and type 1 diabetes (T1D).. In this study, we found a single SH2B3 haplotype significantly associated with increased risk for human T1D.. Fine mapping has demonstrated the most credible causative variant is the single nucleotide rs3184504*T polymorphism in SH2B3.

other:

Article Title: Ambroxol induces myeloma cell death by inhibiting autophagy
Article Snippet: Apoptosis was evaluated by staining with an Annexin V-Fluorescein isothiocyanate (FITC) Apoptosis Detection Kit (Bio Vision, San Francisco, CA) followed by flow cytometric analysis (BD LSR II; Becton Dickinson and Company, Franklin Lakes, NJ).

Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription.
Article Snippet: Cell sorting was performed on a BD FACS Aria or Aria-SORP and cell analysis on a BD LSR Fortessa and BD LSR-II.

Article Title: Autologous Human Dendritic Cells from XDR-TB Patients Polarize a Th1 Response Which Is Bactericidal to Mycobacterium tuberculosis .
Article Snippet: All samples were acquired on a BD LSR-II within 3 days.

Flow Cytometry:

Article Title: Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering.
Article Snippet: Lastly, livers were stained for CD31BV421 (1:20 dilution) and CD326-FITC (1:200 dilution) (BioLegend; San Diego, CA, Fisher Scientific), CD45-BV786 (1:100 dilution) (Becton Dickinson), and ASGR1-CL488 (1:500 dilution) (Thermo Fisher Scientific) to isolate endothelial cells, epithelial cells, immune cells, and hepatocytes, respectively. tdTomato positivity was used to identify cells with successful LoxP stop cassette editing in the Ai9 cassette in thebulkorgans (singlets) aswell as the identified cell type populations. .. Flow cytometry was performed on a BD LSR II (Becton Dickinson) and analyzed via Flowjo V10. ..


Purification:

Article Title: Transient Inflammation of Pancreatic Exocrine Tissue in Autoimmune Diabetes Follows Onset of Islet Damage and Utilizes Heparanase-1.
Article Snippet: .. Data to assess OT cell purification were acquired on a BD LSR II and analyzed using FlowJo software (version 10.0.0, TreeStar, Becton Dickinson, Franklin Lakes, NJ, USA). .. For some experiments designed to study cell migration post-transfer and previously reported [12], OT T cells were labeled with Carboxyfluorescein succinimidyl ester (CFSE) or Cell Trace Violet (CTV) (Molecular Probes, Eugene, OR, USA).

Software:

Article Title: Transient Inflammation of Pancreatic Exocrine Tissue in Autoimmune Diabetes Follows Onset of Islet Damage and Utilizes Heparanase-1.
Article Snippet: .. Data to assess OT cell purification were acquired on a BD LSR II and analyzed using FlowJo software (version 10.0.0, TreeStar, Becton Dickinson, Franklin Lakes, NJ, USA). .. For some experiments designed to study cell migration post-transfer and previously reported [12], OT T cells were labeled with Carboxyfluorescein succinimidyl ester (CFSE) or Cell Trace Violet (CTV) (Molecular Probes, Eugene, OR, USA).



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Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

Journal: bioRxiv

Article Title: A 3-genes interferon signature predicts sustained complete remission in pediatric AML patients

doi: 10.1101/2025.07.17.664572

Figure Lengend Snippet: Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

Article Snippet: Statistical analyses of the flow cytometry data were performed using GraphPad Prism (v. 10.3.1).

Techniques: Flow Cytometry, Biomarker Discovery, Microscopy, Expressing