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fortessa analyzer  (Becton Dickinson)

 
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    Becton Dickinson fortessa analyzer
    Fortessa Analyzer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+data+analyses/fortessa+analyzer/pm34291733-249-6-8
    Average 90 stars, based on 1 article reviews
    fortessa analyzer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Overexpression of AMPKγ2 increases AMPK signaling to augment human T cell metabolism and function
    Article Snippet: Supernatants were assessed for cytokine production using the LegendPlex Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend). .. Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences) and assessed using FlowJo software (version 10.7, Tree Star). .. Where indicated, ELISA to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions.

    Software:

    Article Title: Overexpression of AMPKγ2 increases AMPK signaling to augment human T cell metabolism and function
    Article Snippet: Supernatants were assessed for cytokine production using the LegendPlex Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend). .. Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences) and assessed using FlowJo software (version 10.7, Tree Star). .. Where indicated, ELISA to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions.

    Article Title: A PD‐1 high CD4 + T Cell Population With a Cytotoxic Phenotype is Associated With Interstitial Lung Disease in Systemic Sclerosis
    Article Snippet: .. Data were acquired on a BD Fortessa analyzer using FACSDiva software and analyzed using FlowJo. ..

    Article Title: AMPK drives both glycolytic and oxidative metabolism in murine and human T cells during graft-versus-host disease
    Article Snippet: .. Flow data were captured on a BD Fortessa analyzer (BD Biosciences) and evaluated using FlowJo software (version 10.1, Tree Star). .. A total of 1 × 10 6 T cells were lysed, lysates sonicated and centrifuged to remove insoluble debris, and the remaining lysate incubated with phospho-AMPK Substrate Motif mAb mix (Cell Signaling Technologies; catalog no. 5759) for 12 hours at 4°C.

    other:

    Article Title: Constitutively active CaMKII Drives B lineage acute lymphoblastic leukemia/lymphoma in tp53 mutant zebrafish.
    Article Snippet: Cell populations were incubated with DAPI to eliminate dead cells and analyzed on a Fortessa (Becton-Dickinson, B-D) analyzer or sorted on a FACS Aria II or Fusion Aria high-speed analyzer/sorter (B-D) and saved in either TRIzol reagent (Invitrogen) or 1X PBS + 4% FBS.

    Article Title: Convergent evolution of monocyte differentiation in adult skin permits repair of the Langerhans cell network
    Article Snippet: When required, cells were acquired on a BD Fortessa analzyer equipt with BD FACSDiva software; or sorted into either complete RPMI or RLT lysis buffer (Qiagen) or Trizol using a BD Aria III.



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    Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

    Journal: bioRxiv

    Article Title: A 3-genes interferon signature predicts sustained complete remission in pediatric AML patients

    doi: 10.1101/2025.07.17.664572

    Figure Lengend Snippet: Lymphocyte profiling of the L-TME in pediatric AML patients. (a) Logical gating strategy showing the T cell composition of L-TME in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. The data are displayed cumulatively in the dot plots and individually in the graphs (c and e). The same cumulative and individual representation is depicted in all figures showing flow-cytometry data. (b) I) Cumulative plots displaying CD45 + /CD34 − /CD19 − and CD3 − myeloid cells in AML patients with (top row, n=4 ) and without (bottom row, n=5 ) sustained-CR. II-III) Individual values of samples in (a) and (b) are displayed in a graph; Q value is the result of FDR-corrected multiple t-tests between sustained (red) and non-sustained (black) patients’ samples. Only significant differences are shown. IV) The % of blasts scored in the patients’ diagnosis for all the cohort is displayed in the graph for patients with and without sustained-CR. The blast score was calculated by a combination of microscope and clinical flow-cytometry analyses and provided as clinical report for patients in the Pathology Laboratory of Sidra. (c) I) Phenotype of the CD4 dim cell population: the majority of the cells are IFNγ + (shown in the colored Z-axis) CD4 + and CD8 + doublets, engaging in close networking interactions. A full panel of expression markers for this population is shown in Supplementary Figure 1b. II) Individual values of samples in (b) show a trend of enrichment in CD4 dim doublets (considering lower singlets) in patients without long-term remission.

    Article Snippet: Statistical analyses of the flow cytometry data were performed using GraphPad Prism (v. 10.3.1).

    Techniques: Flow Cytometry, Biomarker Discovery, Microscopy, Expressing