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flow cytometry analysis facs single cell suspensions (Miltenyi Biotec)

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Miltenyi Biotec flow cytometry analysis facs single cell suspensions
Flow Cytometry Analysis Facs Single Cell Suspensions, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 2229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cell/Tumor+Dissociation+Kit%2C+mouse/pm42400909-486-0-13
Average 98 stars, based on 2229 article reviews

flow cytometry analysis facs single cell suspensions - by Bioz Stars, 2026-10

98/100 stars

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Single Cell:

Article Title: Engineering a 660 nm-Responsive Optogenetic Inducer of Pyroptosis for Precision Cancer Therapy.
Article Snippet: .. Single-cell suspensions were prepared using a mouse tumor dissociation kit (Miltenyi Biotec, Cat. 130096730) in accordance with the manufacturer’s protocol, incorporating mechanical disruption via gentleMACS Dissociator (Miltenyi Biotec) and enzymatic digestion at 37◦C for 40 min. .. The resulting suspension was filtered through a 70- μm cell strainer and centrifuged at 300 ×g for 5 min. Erythrocytes were lysed using RBC lysis buffer (eBioscience, Cat. 00433357) for 5 min at RT, followed by two washes.

Disruption:

other:

Article Title: Circulating memory-phenotype CD4 + T cells rapidly accumulate in the ischemic gut to exacerbate tissue injury in an innate manner.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ionomycin Fujifilm Wako Cat# 091-05833 Brefeldin A Solution Biolegend Cat# 420601 Zombie UV Fixable Viability Kit Biolegend Cat# 423108 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 RNase Inhibitor Thermo Fisher Scientific Cat# N8080119 Mouse MOG(35–55) MEVGWYRSPFSRVVHLYRNGK PH Japan NA Bottle M Tuberculosis H37Ra Dessicated BD Biosciences Cat# 231141 Formaldehyde Solution Fujifilm Wako Cat# 061-00411 THUNDERBIRD SYBR qPCR Mix TOYOBO Cat# QPS-201 Dako Target Retrieval Solution Agilent Cat# S1699 HISTOFINE Simple Stain Mouse MAX-PO (Rat) Nichirei Biosciences Cat# 414311 HISTOFINE Simple Stain Mouse MAX-PO (Rabbit) Nichirei Biosciences Cat# 414341 3, 3′-diaminobenzidine tetrahydrochloride Dojindo Laboratories Cat# 349-00903 Histofine Antigen Retrieval Solution (pH 9.0) Nichirei Biosciences Cat# 415211 Histogreen Biozol Cat# E109 O.C.T. compound Sakura Finetek Japan Cat# 4583 Percoll Cytiva Cat# 17089101 Critical commercial assays Lamina Propria Dissociation Kit, mouse Miltenyi Biotec Cat# 130-097-410 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat# 130-096-730 Dead Cell Removal Kit Miltenyi Biotec Cat# 130-090-101 CD4 (L3T4) MicroBeads, mouse Miltenyi Biotec Cat# 130-117-043 CD11b MicroBeads, human and mouse Miltenyi Biotec Cat# 130-097-142 Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit 10x Genomics PN-1000414 Chromium Fixed RNA Kit, Mouse 10x Genomics PN-1000495 RNeasy Mini Kit Qiagen Cat# 74104 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 Mouse TNF-alpha ELISA Kit R&D Systems Cat# MTA00B-1 Mouse IL-12 p70 ELISA Kit R&D Systems Cat# M1270-1 Mouse CXCL1/KC ELISA Kit R&D Systems Cat# MKC00B-1 Mouse CXCL2/MIP-2 ELISA Kit R&D Systems Cat# MM200 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Deposited data Single-cell RNA sequencing data This paper GSE284458 Single-cell RNA sequencing data This paper GSE325534 Single-cell RNA-seq data (Kawabe et al.5) Kawabe et al.5 GSE145999 MSigDB (Molecular Signatures Database) (GOBP_POSITIVE_REGULATION_OF_CD4_ POSITIVE_ALPHA_BETA_T_CELL_ACTIVATION) UC San Diego and Broad Institute https://www.gsea-msigdb. org/gsea/msigdb/ MSigDB (Molecular Signatures Database) (GOBP_POSITIVE_REGULATION_OF_CD4_