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flow cytometry analysis  (Danaher Inc)


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    Structured Review

    Danaher Inc flow cytometry analysis
    Reactivity of 1F3 with cell surface TAZ on membranes of Burkitt's lymphoma, breast cancer and squamous cell carcinoma lines using flow <t>cytometry</t> technique. Values demonstrate the percentages of positive cells. 1F3 detected cell surface expression of TAZ in pathological conditions with no reactivity in normal conditions. Anti-TAZ mAb was applied on: A) A431, B) MCF-7, C) Raji D) HFFF-PI6 E) MDA-MB-231 G) Normal PBMC (anti-TAZ mAb) and anti-TAZ pAb was used on F) A431 (commercial pAb anti TAZ).
    Flow Cytometry Analysis, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+analysis/Exosome+Isolation+and+Analysis+Kit+-+Flow+Cytometry%2C+Cell+culture+(CD63+%2F+CD9)+25+tests/pmc09895982-78-7-28
    Average 99 stars, based on 76 article reviews
    flow cytometry analysis - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "An Anti-TAZ Monoclonal Antibody Recognizing Cell Surface Expressed TAZ Protein in Human Tumor Cells"

    Article Title: An Anti-TAZ Monoclonal Antibody Recognizing Cell Surface Expressed TAZ Protein in Human Tumor Cells

    Journal: Avicenna Journal of Medical Biotechnology

    doi: 10.18502/ajmb.v15i1.11420

    Reactivity of 1F3 with cell surface TAZ on membranes of Burkitt's lymphoma, breast cancer and squamous cell carcinoma lines using flow cytometry technique. Values demonstrate the percentages of positive cells. 1F3 detected cell surface expression of TAZ in pathological conditions with no reactivity in normal conditions. Anti-TAZ mAb was applied on: A) A431, B) MCF-7, C) Raji D) HFFF-PI6 E) MDA-MB-231 G) Normal PBMC (anti-TAZ mAb) and anti-TAZ pAb was used on F) A431 (commercial pAb anti TAZ).
    Figure Legend Snippet: Reactivity of 1F3 with cell surface TAZ on membranes of Burkitt's lymphoma, breast cancer and squamous cell carcinoma lines using flow cytometry technique. Values demonstrate the percentages of positive cells. 1F3 detected cell surface expression of TAZ in pathological conditions with no reactivity in normal conditions. Anti-TAZ mAb was applied on: A) A431, B) MCF-7, C) Raji D) HFFF-PI6 E) MDA-MB-231 G) Normal PBMC (anti-TAZ mAb) and anti-TAZ pAb was used on F) A431 (commercial pAb anti TAZ).

    Techniques Used: Flow Cytometry, Expressing

    Related Articles

    Isolation:

    Article Title: Blockade of exosome generation by GW4869 inhibits the education of M2 macrophages in prostate cancer
    Article Snippet: .. We followed the instructions of the Exosome Isolation and Analysis Kit-Flow Cytometry (ab267478, Abcam). ..

    Cytometry:

    Article Title: Blockade of exosome generation by GW4869 inhibits the education of M2 macrophages in prostate cancer
    Article Snippet: .. We followed the instructions of the Exosome Isolation and Analysis Kit-Flow Cytometry (ab267478, Abcam). ..

    Flow Cytometry:

    Article Title: Engineering CpG-ASO-Pt-Loaded Macrophages (CAP@M) for Synergistic Chemo-/Gene-/Immuno-Therapy
    Article Snippet: .. For flow cytometry analysis, the cells were collected and stained with PE-conjugated mouse antihuman P-gp antibody (1:50, ab93590, Abcam, UK). .. The amounts of fluorescent signal were quantified using flow cytometry system (LSRFortessa, BD, USA).

    Article Title: An Anti-TAZ Monoclonal Antibody Recognizing Cell Surface Expressed TAZ Protein in Human Tumor Cells
    Article Snippet: A polyclonal anti-TAZ antibody (Abcam, UK) was used as standard control. β-actin antibody was used as an internal control (Padzaco). .. To evaluate the localization of TAZ protein, flow cytometry analysis was performed both at intracellular and cell surface staining using both produced anti-TAZ mAb and polyclonal anti-TAZ antibody (Abcam). ..

    Staining:

    Article Title: Engineering CpG-ASO-Pt-Loaded Macrophages (CAP@M) for Synergistic Chemo-/Gene-/Immuno-Therapy
    Article Snippet: .. For flow cytometry analysis, the cells were collected and stained with PE-conjugated mouse antihuman P-gp antibody (1:50, ab93590, Abcam, UK). .. The amounts of fluorescent signal were quantified using flow cytometry system (LSRFortessa, BD, USA).

    Article Title: An Anti-TAZ Monoclonal Antibody Recognizing Cell Surface Expressed TAZ Protein in Human Tumor Cells
    Article Snippet: A polyclonal anti-TAZ antibody (Abcam, UK) was used as standard control. β-actin antibody was used as an internal control (Padzaco). .. To evaluate the localization of TAZ protein, flow cytometry analysis was performed both at intracellular and cell surface staining using both produced anti-TAZ mAb and polyclonal anti-TAZ antibody (Abcam). ..

    Western Blot:

    Article Title: Low-intensity pulsed ultrasound (LIPUS) enhances the anti-inflammatory effects of bone marrow mesenchymal stem cells (BMSCs)-derived extracellular vesicles
    Article Snippet: .. The characteristic proteins of EVs including CD9, CD63, CD81 and TSG101 (Abcam, UK) were analyzed by western blotting. ..

    Article Title: Cartilage fragments combined with BMSCs-Derived exosomes can promote tendon-bone healing after ACL reconstruction
    Article Snippet: Morphological characteristics of BMSCs-Exos were determined via transmission electron microscope (TEM) (JEM-1400, JEOL, Japan). .. Western blot was performed to confirm Exos markers, including Alix (Cell Signaling Technology, USA), TSG101 (Protein Tech, USA), and CD9 (Abcam, USA). .. The size distribution of BMSCs-Exos was determined using qNano analysis (Malvern, UK).

    Article Title: Breast cancer stem cell-derived extracellular vesicles transfer ARRDC1-AS1 to promote breast carcinogenesis via a miR-4731-5p/AKT1 axis-dependent mechanism
    Article Snippet: The size distribution of EVs was determined using Nanosight nanoparticle tracking analyzer (Malvern Panalytical, Malvern, UK) by nanoparticle tracking analysis (NTA). .. The expression of surface markers of EVs (CD63, CD81 and TSG101) and non-EVs (Calnexin) was determined using Western blot analysis based on such antibodies as TSG101 (ab30871, Abcam, Cambridge, UK), CD81 (ab92726, Abcam), CD63 (ab134045, Abcam) and Calnexin (ab133615, Abcam). .. The morphology of EVs was characterized by a transmission electron microscope (TEM) (JEM-1010, JEOL, Tokyo, Japan) .

    Incubation:

    Article Title: An exosome-based gene delivery platform for cell-specific CRISPR/Cas9 genome editing
    Article Snippet: The exosome isolated fraction and EV-depleted fraction were frozen and stored at -80°C until further use. .. The exosomes were incubated with capture beads for CD81 (ab239687, ABCAM) and CD63 (ab239686, ABCAM) overnight at 4 °C, as recommended by the manufacturer. ..

    Expressing:

    Article Title: Continuous nutrient supply culture strategy controls multivesicular endosomes pathway and anti-photo-aging miRNA cargo loading of extracellular vesicles
    Article Snippet: .. Finally, the total protein extract (40 μg) was separated and the protein expression of Protein 1A/1B-light chain I/II (LC3 I/II), PARP (ZenBioScience, China), CD9, CD63 and CD81 (Abcam, UK) were examined according to the previous method. ..

    Article Title: Breast cancer stem cell-derived extracellular vesicles transfer ARRDC1-AS1 to promote breast carcinogenesis via a miR-4731-5p/AKT1 axis-dependent mechanism
    Article Snippet: The size distribution of EVs was determined using Nanosight nanoparticle tracking analyzer (Malvern Panalytical, Malvern, UK) by nanoparticle tracking analysis (NTA). .. The expression of surface markers of EVs (CD63, CD81 and TSG101) and non-EVs (Calnexin) was determined using Western blot analysis based on such antibodies as TSG101 (ab30871, Abcam, Cambridge, UK), CD81 (ab92726, Abcam), CD63 (ab134045, Abcam) and Calnexin (ab133615, Abcam). .. The morphology of EVs was characterized by a transmission electron microscope (TEM) (JEM-1010, JEOL, Tokyo, Japan) .

    Produced:

    Article Title: An Anti-TAZ Monoclonal Antibody Recognizing Cell Surface Expressed TAZ Protein in Human Tumor Cells
    Article Snippet: A polyclonal anti-TAZ antibody (Abcam, UK) was used as standard control. β-actin antibody was used as an internal control (Padzaco). .. To evaluate the localization of TAZ protein, flow cytometry analysis was performed both at intracellular and cell surface staining using both produced anti-TAZ mAb and polyclonal anti-TAZ antibody (Abcam). ..



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    (A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow <t>cytometry</t> at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .
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    Image Search Results


    (A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow cytometry at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .

    Journal: PLOS Pathogens

    Article Title: Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients

    doi: 10.1371/journal.ppat.1014542

    Figure Lengend Snippet: (A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow cytometry at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .

    Article Snippet: Samples for flow cytometry analysis (using a CytoFLEX LX flow cytometer, Beckman Coulter) were prepared by resuspending parasite cultures (1:3) in PBS containing 1% BSA, followed by staining with Hoechst 33342 (1:500, BD Pharmingen) in a final volume of 100 μl per well.

    Techniques: In Vitro, Cell Culture, Parasite, Flow Cytometry, Control

    (A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA n = 1, HbAC n = 1 and HbAS n = 2, and HbSS n = 2 and HbSC n = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages ( i.e., schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( n = 5), HbAC ( n = 5),HbAS ( n = 4), and HbSC ( n = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS ( n = 12), HbSC ( n = 1) and n = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.

    Journal: PLOS Pathogens

    Article Title: Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients

    doi: 10.1371/journal.ppat.1014542

    Figure Lengend Snippet: (A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA n = 1, HbAC n = 1 and HbAS n = 2, and HbSS n = 2 and HbSC n = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages ( i.e., schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( n = 5), HbAC ( n = 5),HbAS ( n = 4), and HbSC ( n = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS ( n = 12), HbSC ( n = 1) and n = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.

    Article Snippet: Samples for flow cytometry analysis (using a CytoFLEX LX flow cytometer, Beckman Coulter) were prepared by resuspending parasite cultures (1:3) in PBS containing 1% BSA, followed by staining with Hoechst 33342 (1:500, BD Pharmingen) in a final volume of 100 μl per well.

    Techniques: Microscopy, Parasite, Infection, Flow Cytometry, Control