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Structured Review

Becton Dickinson flow cytometry analysis software
Flow Cytometry Analysis Software, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+analysis/flow+cytometry+analysis+software/pmc05414608-107-5-5
Average 90 stars, based on 1 article reviews
flow cytometry analysis software - by Bioz Stars, 2026-11
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Related Articles

Staining:

Article Title: Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells
Article Snippet: .. After staining, samples were read with an LSR II FACS/flow cytometer (BD Biosciences, San Jose, CA) and analyzed with FlowJo flow cytometry software (v.10; Becton, Dickinson & Company, Ashland, OR). ..

Article Title: Immunophenotyping with (phospho)protein profiling and fluorescent cell barcoding for single-cell signaling analysis and biomarker discovery
Article Snippet: .. Import the Flow Cytometry Standard (FCS) data files from the experiment to a flow cytometry analysis software, i.e., FlowJo (Ashland, Oregon, USA) or Cytobank (Mountain View, California, USA) Gating strategy: Identification of lymphocytes and FCB populations Select events collected under consistent flow rate signal by plotting time versus a fluorochrome on the UV laser (e.g. anti-CD3) (Fig. ) Fig. 2 Gating strategy for immunophenotyping of peripheral blood mononuclear cells from healthy donors. a Peripheral blood mononuclear cells (PBMCs) from a healthy donor were stained with surface markers and analysed according to the protocol using a Cytek 5 L Aurora instrument. ..

FACS:

Article Title: Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells
Article Snippet: .. After staining, samples were read with an LSR II FACS/flow cytometer (BD Biosciences, San Jose, CA) and analyzed with FlowJo flow cytometry software (v.10; Becton, Dickinson & Company, Ashland, OR). ..

Cytometry:

Article Title: Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells
Article Snippet: .. After staining, samples were read with an LSR II FACS/flow cytometer (BD Biosciences, San Jose, CA) and analyzed with FlowJo flow cytometry software (v.10; Becton, Dickinson & Company, Ashland, OR). ..

Flow Cytometry:

Article Title: Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells
Article Snippet: .. After staining, samples were read with an LSR II FACS/flow cytometer (BD Biosciences, San Jose, CA) and analyzed with FlowJo flow cytometry software (v.10; Becton, Dickinson & Company, Ashland, OR). ..

Article Title: Immunophenotyping with (phospho)protein profiling and fluorescent cell barcoding for single-cell signaling analysis and biomarker discovery
Article Snippet: .. Import the Flow Cytometry Standard (FCS) data files from the experiment to a flow cytometry analysis software, i.e., FlowJo (Ashland, Oregon, USA) or Cytobank (Mountain View, California, USA) Gating strategy: Identification of lymphocytes and FCB populations Select events collected under consistent flow rate signal by plotting time versus a fluorochrome on the UV laser (e.g. anti-CD3) (Fig. ) Fig. 2 Gating strategy for immunophenotyping of peripheral blood mononuclear cells from healthy donors. a Peripheral blood mononuclear cells (PBMCs) from a healthy donor were stained with surface markers and analysed according to the protocol using a Cytek 5 L Aurora instrument. ..

Article Title: B cell senescence promotes age-related changes in oral microbiota.
Article Snippet: .. Analysis of flow cytometry data was performed on FlowJo version 10.8.1 software (Beckton Dickinson). .. DNA extraction from saliva samples was performed using PureLinkTM Genomic DNA Mini Kit (K182002; Thermo Fisher SCIENTIFIC) according to the manufacture's protocol.

Software:

Article Title: Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells
Article Snippet: .. After staining, samples were read with an LSR II FACS/flow cytometer (BD Biosciences, San Jose, CA) and analyzed with FlowJo flow cytometry software (v.10; Becton, Dickinson & Company, Ashland, OR). ..

Article Title: Immunophenotyping with (phospho)protein profiling and fluorescent cell barcoding for single-cell signaling analysis and biomarker discovery
Article Snippet: .. Import the Flow Cytometry Standard (FCS) data files from the experiment to a flow cytometry analysis software, i.e., FlowJo (Ashland, Oregon, USA) or Cytobank (Mountain View, California, USA) Gating strategy: Identification of lymphocytes and FCB populations Select events collected under consistent flow rate signal by plotting time versus a fluorochrome on the UV laser (e.g. anti-CD3) (Fig. ) Fig. 2 Gating strategy for immunophenotyping of peripheral blood mononuclear cells from healthy donors. a Peripheral blood mononuclear cells (PBMCs) from a healthy donor were stained with surface markers and analysed according to the protocol using a Cytek 5 L Aurora instrument. ..

Article Title: B cell senescence promotes age-related changes in oral microbiota.
Article Snippet: .. Analysis of flow cytometry data was performed on FlowJo version 10.8.1 software (Beckton Dickinson). .. DNA extraction from saliva samples was performed using PureLinkTM Genomic DNA Mini Kit (K182002; Thermo Fisher SCIENTIFIC) according to the manufacture's protocol.

other:

Article Title: In vitro biocompatibility of polycaprolactone/hydroxyapatite nanocomposite membranes modified by oleic acid for bone regeneration
Article Snippet: A R T I C L E I N F O

Article Title: Pro-tumorigenic activity of PYCR1 in gastric cancer through regulating the PI3K/AKT signaling
Article Snippet: A FACS Calibur equipment was utilized to conduct the flow cytometric analysis, and the acquired data were Assessed using FlowJo7.6 software (FlowJo LLC, Ashland, OR).

Article Title: Modulating efficacy and cytotoxicity of lipoamino fatty acid nucleic acid carriers using disulfide or hydrophobic spacers.
Article Snippet: Flow cytometry data were analyzed using FlowJoTM v10.8 flow cytometric analysis software by FlowJo, LLC (Becton, 20 Dickinson and Company, U.S.).

Article Title: NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K-Akt-mTOR signaling pathway
Article Snippet: Cell cycle progression was assessed using a flow cytometer (FACSCalibur; BD Biosciences), and the proportion of cells in each phase of the cell cycle was analyzed using FlowJo7.6 software (FlowJo LLC).

Article Title: Early tolerance and late persistence as alternative drug responses in cancer
Article Snippet: Immediately after transfection cells were treated with FOLFOX (5-FU 100 μM, oxaliplatin 100 μM) or FOLFIRI (5-FU 100 μM, SN38 100 nM) for 16 h and with ULK1 inhibitor (100 μM), then harvested, acquired by flow cytometry at CytoFLEX LX (Beckman Coulter) and analysed by FlowJo (Becton Dickinson v10.8.1).



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(A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow <t>cytometry</t> at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .
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(A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow <t>cytometry</t> at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .
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Image Search Results


(A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow cytometry at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .

Journal: PLOS Pathogens

Article Title: Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients

doi: 10.1371/journal.ppat.1014542

Figure Lengend Snippet: (A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow cytometry at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .

Article Snippet: Samples for flow cytometry analysis (using a CytoFLEX LX flow cytometer, Beckman Coulter) were prepared by resuspending parasite cultures (1:3) in PBS containing 1% BSA, followed by staining with Hoechst 33342 (1:500, BD Pharmingen) in a final volume of 100 μl per well.

Techniques: In Vitro, Cell Culture, Parasite, Flow Cytometry, Control

(A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA n = 1, HbAC n = 1 and HbAS n = 2, and HbSS n = 2 and HbSC n = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages ( i.e., schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( n = 5), HbAC ( n = 5),HbAS ( n = 4), and HbSC ( n = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS ( n = 12), HbSC ( n = 1) and n = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.

Journal: PLOS Pathogens

Article Title: Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients

doi: 10.1371/journal.ppat.1014542

Figure Lengend Snippet: (A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA n = 1, HbAC n = 1 and HbAS n = 2, and HbSS n = 2 and HbSC n = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages ( i.e., schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( n = 5), HbAC ( n = 5),HbAS ( n = 4), and HbSC ( n = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS ( n = 12), HbSC ( n = 1) and n = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.

Article Snippet: Samples for flow cytometry analysis (using a CytoFLEX LX flow cytometer, Beckman Coulter) were prepared by resuspending parasite cultures (1:3) in PBS containing 1% BSA, followed by staining with Hoechst 33342 (1:500, BD Pharmingen) in a final volume of 100 μl per well.

Techniques: Microscopy, Parasite, Infection, Flow Cytometry, Control