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imaging flow cytometry analysis  (GraphPad Software Inc)


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    Structured Review

    GraphPad Software Inc imaging flow cytometry analysis
    Imaging Flow Cytometry Analysis, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+analysis/flow+cytometry+data+analysis+software/pm39486562-115-0-18
    Average 90 stars, based on 1 article reviews
    imaging flow cytometry analysis - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Combining ablative radiotherapy and anti CD47 monoclonal antibody improves infiltration of immune cells in tumor microenvironments.
    Article Snippet: .. Then, the final flow cytometry data were analyzed with GraphPad Prism version 8. ..

    Article Title: Human Hepatic CD56 bright NK Cells Display a Tissue-Resident Transcriptional Profile and Enhanced Ability to Kill Allogenic CD8 + T Cells
    Article Snippet: .. Statistical analysis of flow cytometry data was performed using GraphPad Prism version 9. ..

    Article Title: Imaging flow cytometry reveals LPS-induced changes to intracellular intensity and distribution of α-synuclein in a TLR4-dependent manner in STC-1 cells.
    Article Snippet: .. Imaging flow cytometry analysis After gating, geometric means for each feature for every included cell were imported into GraphPad Prism (v9.2). ..

    Article Title: Delineating the transcriptional landscape and clonal diversity of virus-specific CD4 + T cells during chronic viral infection
    Article Snippet: .. Statistical tests for flow cytometry data were performed using Graphpad Prism 8. p Values were calculated using two-tailed unpaired Student’s t-tests. p Values for violin plots showing gene set-specific module scores across clusters were calculated by the Wilcoxon test with Holm-Sidak correction. ..

    Article Title: Delineating the transcriptional landscape and clonal diversity of virus-specific CD4+ T cells during chronic viral infection
    Article Snippet: Statistical tests for flow cytometry data were performed using Graphpad Prism 8. p Values were calculated using two- tailed unpaired Student’s t- tests. p Values for violin plots showing gene set- specific module scores across clusters were calculated by the Wilcoxon test with Holm- Sidak correction. .. Statistical tests for flow cytometry data were performed using Graphpad Prism 8. p Values were calculated using two- tailed unpaired Student’s t- tests. p Values for violin plots showing gene set- specific module scores across clusters were calculated by the Wilcoxon test with Holm- Sidak correction. ..

    other:

    Article Title: Acasunlimab, an Fc-inert PD-L1×4-1BB bispecific antibody, combined with PD-1 blockade potentiates antitumor immunity via complementary immune modulatory effects.
    Article Snippet: 10.1136/jitc-2024-011377 o n J Im m u n o th er C an cer: first p u b lish ed as 5Capello M, et al. J Immunother Cancer 2025;13:e011377. doi:10.1136/jitc-2024-011377 in which the combination of agents demonstrated significant superiority (p<0.05) relative to the activity shown by each agent alone.33 For IHC, flow cytometry, and cytokine readouts, differences between treatment groups were analyzed using Mann- Whitney or Kruskal- Wallis analysis in GraphPad Prism.

    Article Title: Haploinsufficiency of immune checkpoint receptor CTLA4 induces a distinct neuroinflammatory disorder
    Article Snippet: Immunohistochemical staining was performed using an Ultra automated immunostainer (Roche) according to the manufacturer’s instructions.

    Article Title: Anti-Cancer Activity of PAK4/NAMPT Inhibitor and Programmed Cell Death Protein-1 Antibody in Kidney Cancer
    Article Snippet: The quantitative methylation specific PCR (qMSP) reactions were performed on 100% methylated and untreated human gDNA using Power SYBR Green Master Mix (ThermoFisher) on 1 μ l of bisulfite-converted gDNA with 400 nM of primers specific for methylated (HUMAN) NAPRT or 250 nM of (HUMAN) ACTB primers.

    Imaging:

    Article Title: Imaging flow cytometry reveals LPS-induced changes to intracellular intensity and distribution of α-synuclein in a TLR4-dependent manner in STC-1 cells.
    Article Snippet: .. Imaging flow cytometry analysis After gating, geometric means for each feature for every included cell were imported into GraphPad Prism (v9.2). ..

    Two Tailed Test:

    Article Title: Delineating the transcriptional landscape and clonal diversity of virus-specific CD4+ T cells during chronic viral infection
    Article Snippet: Statistical tests for flow cytometry data were performed using Graphpad Prism 8. p Values were calculated using two- tailed unpaired Student’s t- tests. p Values for violin plots showing gene set- specific module scores across clusters were calculated by the Wilcoxon test with Holm- Sidak correction. .. Statistical tests for flow cytometry data were performed using Graphpad Prism 8. p Values were calculated using two- tailed unpaired Student’s t- tests. p Values for violin plots showing gene set- specific module scores across clusters were calculated by the Wilcoxon test with Holm- Sidak correction. ..



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    Image Search Results


    (A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow cytometry at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .

    Journal: PLOS Pathogens

    Article Title: Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients

    doi: 10.1371/journal.ppat.1014542

    Figure Lengend Snippet: (A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow cytometry at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .

    Article Snippet: Samples for flow cytometry analysis (using a CytoFLEX LX flow cytometer, Beckman Coulter) were prepared by resuspending parasite cultures (1:3) in PBS containing 1% BSA, followed by staining with Hoechst 33342 (1:500, BD Pharmingen) in a final volume of 100 μl per well.

    Techniques: In Vitro, Cell Culture, Parasite, Flow Cytometry, Control

    (A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA n = 1, HbAC n = 1 and HbAS n = 2, and HbSS n = 2 and HbSC n = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages ( i.e., schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( n = 5), HbAC ( n = 5),HbAS ( n = 4), and HbSC ( n = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS ( n = 12), HbSC ( n = 1) and n = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.

    Journal: PLOS Pathogens

    Article Title: Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients

    doi: 10.1371/journal.ppat.1014542

    Figure Lengend Snippet: (A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA n = 1, HbAC n = 1 and HbAS n = 2, and HbSS n = 2 and HbSC n = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages ( i.e., schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( n = 5), HbAC ( n = 5),HbAS ( n = 4), and HbSC ( n = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS ( n = 12), HbSC ( n = 1) and n = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.

    Article Snippet: Samples for flow cytometry analysis (using a CytoFLEX LX flow cytometer, Beckman Coulter) were prepared by resuspending parasite cultures (1:3) in PBS containing 1% BSA, followed by staining with Hoechst 33342 (1:500, BD Pharmingen) in a final volume of 100 μl per well.

    Techniques: Microscopy, Parasite, Infection, Flow Cytometry, Control