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streptavidin coated flashplate  (Thermo Fisher)


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    Structured Review

    Thermo Fisher streptavidin coated flashplate
    Streptavidin Coated Flashplate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flashplate/Streptavidin/pmc11538787-259-8-36
    Average 99 stars, based on 1 article reviews
    streptavidin coated flashplate - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Protein Concentration:

    Article Title: OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy
    Article Snippet: .. In some experiments, bio-AddAB was pre-incubated with streptavidin-conjugated Qdot525 (Thermo Fisher Scientific, Q10143MP) at a 1:2 (AddAB:Qdot) molar ratio for 10 min on ice before dilution with experimental buffer to a final protein concentration of 10 nM (referred to as QD525-AddAB). ..

    Transfection:

    Article Title: Interacting proteins of AMPK studied using TurboID proximity labeling technology
    Article Snippet: The streptavidin-peroxidase antibody (cat. no. SA00001-0) was obtained from Proteintech Group, Inc. CCCP (cat. no. C6700) was purchased from Beijing Solarbio Science & Technology Co., Ltd. PVDF membranes were obtained from MilliporeSigma. .. Dynabeads TM MyOne TM streptavidin C1 (cat. no. 65001), biotin (cat. no. B20656 ) and liposomal transfection reagent (Lipofectamine ® 2000; cat. no. 11668500) were obtained from Thermo Fisher Scientific, Inc. RIPA buffer (cat. no. G2002-100ML), DAPI (cat. no. G1012-100ML) and anti-fluorescence quenching sealing agent (cat. no. G1401-25ML) were purchased from Wuhan Servicebio Technology Co., Ltd. .. The anti-DYKDDDDK (Flag) affinity gel (cat. no. 20585ES03), lentivirus concentration solution (cat. no. 41101ES50) and protein silver staining kit (cat. no. 36244ES25) were purchased from Shanghai Yeasen Biotechnology Co., Ltd. A DNA purification kit (cat. no. DP204) and a high purity plasmid extraction kit (cat. no. DP104) were obtained from Tiangen Biotech Co., Ltd. Lastly, inorganic salts were purchased from Sinopharm Chemical Reagent Co., Ltd.

    Article Title: CircEif3c/miR-96–5p/PHF20L1/MEOX2 axis in perivascular preadipocyte exosomes mediates fibroblast dysfunction and vascular remodeling
    Article Snippet: .. Cell lysates were collected 48 h after transfection and incubated with streptavidin magnetic beads (#65306, Invitrogen, USA) for 2 h. After centrifugation to wash the beads, the pulled-down miRNAs were extracted using Trizol (#15596018, Invitrogen, USA) and subjected to qRT-PCR. ..

    Incubation:

    Article Title: A conserved mammalian mecciRNA, mecciATP6, regulates mitochondrial homeostasis through interaction with HNRNPA3
    Article Snippet: .. The supernatant was collected, and 100 pmol of biotinylated antisense oligonucleotide was added, followed by incubation at room temperature for 2 h. Concurrently, M-280 streptavidin magnetic beads (Life Technologies) were blocked by incubating with 500 ng/μL yeast tRNA and 500 ng/μL BSA at room temperature for 1 h, washed with RIPA buffer, and resuspended. .. For every 100 pmol of biotin-DNA oligonucleotide, 50 μL of the blocked beads were added to the mixture, which was then incubated with rotation at room temperature for 2 h. The beads were collected using a magnetic stand (Life Technologies) and washed twice with RIPA buffer, followed by two washes with RIPA buffer supplemented with 500 mmol/L NaCl.

    Article Title: CircEif3c/miR-96–5p/PHF20L1/MEOX2 axis in perivascular preadipocyte exosomes mediates fibroblast dysfunction and vascular remodeling
    Article Snippet: .. Cell lysates were collected 48 h after transfection and incubated with streptavidin magnetic beads (#65306, Invitrogen, USA) for 2 h. After centrifugation to wash the beads, the pulled-down miRNAs were extracted using Trizol (#15596018, Invitrogen, USA) and subjected to qRT-PCR. ..

    Article Title: HSF1 and YTHDC1 enhance DNA repair under heat stress by promoting repair-associated gene expression
    Article Snippet: .. In addition, cells were incubated with streptavidin 488 (Invitrogen, S11223 ) simultaneously with secondary antibodies. .. Images were acquired using a Leica STELLARIS 5 microscope or a Leica TCS SP8 STED 3X microscope for high-resolution STED imaging.

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Samples were acquired on a BD LSRFortessa flow cytometer. .. Biotinylated circSMAD4 sense RNA (with antisense RNA as a negative control) was generated by in vitro transcription using Biotin-16-UTP (Beyotime, Cat# D7336), incubated with macrophage lysates, and captured using streptavidin beads (Invitrogen, Cat# 88816). ..

    Magnetic Beads:

    Article Title: A conserved mammalian mecciRNA, mecciATP6, regulates mitochondrial homeostasis through interaction with HNRNPA3
    Article Snippet: .. The supernatant was collected, and 100 pmol of biotinylated antisense oligonucleotide was added, followed by incubation at room temperature for 2 h. Concurrently, M-280 streptavidin magnetic beads (Life Technologies) were blocked by incubating with 500 ng/μL yeast tRNA and 500 ng/μL BSA at room temperature for 1 h, washed with RIPA buffer, and resuspended. .. For every 100 pmol of biotin-DNA oligonucleotide, 50 μL of the blocked beads were added to the mixture, which was then incubated with rotation at room temperature for 2 h. The beads were collected using a magnetic stand (Life Technologies) and washed twice with RIPA buffer, followed by two washes with RIPA buffer supplemented with 500 mmol/L NaCl.

    Article Title: CircEif3c/miR-96–5p/PHF20L1/MEOX2 axis in perivascular preadipocyte exosomes mediates fibroblast dysfunction and vascular remodeling
    Article Snippet: .. Cell lysates were collected 48 h after transfection and incubated with streptavidin magnetic beads (#65306, Invitrogen, USA) for 2 h. After centrifugation to wash the beads, the pulled-down miRNAs were extracted using Trizol (#15596018, Invitrogen, USA) and subjected to qRT-PCR. ..

    Centrifugation:

    Article Title: CircEif3c/miR-96–5p/PHF20L1/MEOX2 axis in perivascular preadipocyte exosomes mediates fibroblast dysfunction and vascular remodeling
    Article Snippet: .. Cell lysates were collected 48 h after transfection and incubated with streptavidin magnetic beads (#65306, Invitrogen, USA) for 2 h. After centrifugation to wash the beads, the pulled-down miRNAs were extracted using Trizol (#15596018, Invitrogen, USA) and subjected to qRT-PCR. ..

    Negative Control:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Samples were acquired on a BD LSRFortessa flow cytometer. .. Biotinylated circSMAD4 sense RNA (with antisense RNA as a negative control) was generated by in vitro transcription using Biotin-16-UTP (Beyotime, Cat# D7336), incubated with macrophage lysates, and captured using streptavidin beads (Invitrogen, Cat# 88816). ..

    Generated:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Samples were acquired on a BD LSRFortessa flow cytometer. .. Biotinylated circSMAD4 sense RNA (with antisense RNA as a negative control) was generated by in vitro transcription using Biotin-16-UTP (Beyotime, Cat# D7336), incubated with macrophage lysates, and captured using streptavidin beads (Invitrogen, Cat# 88816). ..

    In Vitro:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Samples were acquired on a BD LSRFortessa flow cytometer. .. Biotinylated circSMAD4 sense RNA (with antisense RNA as a negative control) was generated by in vitro transcription using Biotin-16-UTP (Beyotime, Cat# D7336), incubated with macrophage lysates, and captured using streptavidin beads (Invitrogen, Cat# 88816). ..



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