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Huabio Inc antibodies to flag
Antibodies To Flag, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag/anti+flag/pm42277940-152-1-11
Average 86 stars, based on 1 article reviews
antibodies to flag - by Bioz Stars, 2026-11
86/100 stars

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Article Title: DNA-contact mutant p53 displaces BRCA2 from chromatin and drives R-loop-associated genome instability.
Article Snippet: The antibodies to FLAG (HA601080) and RAD51 (ET1705-9) were obtained from HUABIO.

Article Title: Mechanisms of TRIM21-mediated RUNX2 degradation via chaperone-mediated autophagy in periodontitis.
Article Snippet: Protein expression levels were quantified by assessing bands intensities with ImageJ software 5.0 (NIH, Bethesda, MD, USA).

Article Title: STING Degradation by PRRSV Activates HK 2 -Mediated Glycolysis to Facilitate Viral Replication
Article Snippet: The antibodies used in this study included anti-rabbit STING (50494S; Cell Signaling Technology, Danvers, MA, USA), anti-Flag (HA722780; HUABIO, Hangzhou, China), anti-GFP (ET1607-31; HUABIO,), anti-PRRSV-N (GTX642546; GeneTex, San Antonio, TX, USA), anti-β-tubulin (GB152667-100; Servicebio, Wuhan, China), and anti-mouse HK 2 (66974-1-Ig, Proteintech, Rosemont, IL, USA).

Article Title: Virtual and CMC-Based Screening Identified Reticuline, an Intermediate of BIA Biosynthesis, as a Potential Agonist of D5R
Article Snippet: Next, 50 mg silica gel carriers (SiO 2 -NH 2 ) (Acchorm, Beijing, China), which were modified with FLAG-tag-specific antibodies (Huabio, Hangzhou, China), were incubated with the cell membrane suspension at 37 °C for 1 h at an agitation speed of 1400 rpm.

Incubation:

Article Title: Structural and evolutionary insights into the eukaryotic RNase MRP ribonucleoprotein complex.
Article Snippet: Proteins were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, # IPVH00010). .. The membranes were blocked with a solution containing 5% BSA in PBS buffer supplemented with 0.05% TWEEN-20 at room temperature for 1 hr, and then incubated with anti-Flag antibody (HUABIO, #0912- 1) at dilution of 1:5,000 and anti-Tubulin antibody (Proteintech, 14555-1-AP) at dilution of 1:20,000 in blocking buffer at 4 °C overnight. ..

Article Title: Adaptive evolution of endothelial nitric oxide synthase (NOS3) may reduce cetacean susceptibility to decompression sickness.
Article Snippet: .. Cell lysates were 29 AC CE PT ED M AN US CR IP T D ow nloaded from https://academ ic.oup.com /m be/advance-article/doi/10.1093/m olbev/m sag144/8706617 by U niversidade de Brasilia user on 16 June 2026 incubated with anti-FLAG magnetic beads (HUABIO, Hangzhou, China) at 4 °C overnight. ..

Blocking Assay:

Article Title: Structural and evolutionary insights into the eukaryotic RNase MRP ribonucleoprotein complex.
Article Snippet: Proteins were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, # IPVH00010). .. The membranes were blocked with a solution containing 5% BSA in PBS buffer supplemented with 0.05% TWEEN-20 at room temperature for 1 hr, and then incubated with anti-Flag antibody (HUABIO, #0912- 1) at dilution of 1:5,000 and anti-Tubulin antibody (Proteintech, 14555-1-AP) at dilution of 1:20,000 in blocking buffer at 4 °C overnight. ..

Magnetic Beads:

Article Title: Adaptive evolution of endothelial nitric oxide synthase (NOS3) may reduce cetacean susceptibility to decompression sickness.
Article Snippet: .. Cell lysates were 29 AC CE PT ED M AN US CR IP T D ow nloaded from https://academ ic.oup.com /m be/advance-article/doi/10.1093/m olbev/m sag144/8706617 by U niversidade de Brasilia user on 16 June 2026 incubated with anti-FLAG magnetic beads (HUABIO, Hangzhou, China) at 4 °C overnight. ..

Immunoprecipitation:

Article Title: Sarsasapogenin ameliorates Alzheimer's disease by dual inhibition of RIPK1-mediated necroptosis and pyroptosis.
Article Snippet: Sarsasapogenin (Sar), a natural bioactive steroidal saponin derived from Anemarrhena asphodeloides, has demonstrated significant neuroprotective effects in preclinical models of Alzheimer's disease (AD).. However, its specific mechanism of action, particularly in modulating receptor-interacting protein kinase 1 (RIPK1)-mediated necroptosis and pyroptosis, remains underexplored.. This study aimed to investigate Sar's therapeutic potential in AD by targeting RIPK1, a central regulator of programmed cell death.

Lysis:

Article Title: Sarsasapogenin ameliorates Alzheimer's disease by dual inhibition of RIPK1-mediated necroptosis and pyroptosis.
Article Snippet: Sarsasapogenin (Sar), a natural bioactive steroidal saponin derived from Anemarrhena asphodeloides, has demonstrated significant neuroprotective effects in preclinical models of Alzheimer's disease (AD).. However, its specific mechanism of action, particularly in modulating receptor-interacting protein kinase 1 (RIPK1)-mediated necroptosis and pyroptosis, remains underexplored.. This study aimed to investigate Sar's therapeutic potential in AD by targeting RIPK1, a central regulator of programmed cell death.



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Image Search Results


Journal: eLife

Article Title: p66Shc mediates SUMO2-induced endothelial dysfunction

doi: 10.7554/eLife.106344

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Flag-SENP1 , Dr. Edward Yeh , Addgene plasmid # 17357 , .

Techniques: Recombinant, Plasmid Preparation