dbp lentiviruses expressing the flag peptide (lv-flag-dbp and control lv-flag) (Shanghai GenePharma)
90
Structured Review
Shanghai GenePharma
dbp lentiviruses expressing the flag peptide (lv-flag-dbp and control lv-flag)
Dbp Lentiviruses Expressing The Flag Peptide (Lv Flag Dbp And Control Lv Flag), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+control/dbp+lentiviruses+expressing+the+flag+peptide++lv+flag+dbp+and+control+lv+flag+/pmc06804544-307-0-20
Average 90 stars, based on 1 article reviews
Dbp Lentiviruses Expressing The Flag Peptide (Lv Flag Dbp And Control Lv Flag), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+control/dbp+lentiviruses+expressing+the+flag+peptide++lv+flag+dbp+and+control+lv+flag+/pmc06804544-307-0-20
Average 90 stars, based on 1 article reviews
dbp lentiviruses expressing the flag peptide (lv-flag-dbp and control lv-flag) - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis"
Article Title: The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis
Journal: Cellular and Molecular Immunology
doi: 10.1038/s41423-018-0020-4
Figure Legend Snippet: DBP regulated CCL2 expression via the target gene TRIM55. a siDBP inhibited TRIM55 mRNA expression, but not the expression of six other genes. b DBP bound to the TRIM55 promoter, as revealed by chromatin immunoprecipitation sequencing (ChIP)-PCR. c DBP bound to the TRIM55 promoter (-2984 bp), as revealed by the dual luciferase assay. d The TRIM55 promoter was divided into three subregions. e The dual luciferase assay indicated that DBP specifically bound to the -2116–2984-bp region. f, g: siTRIM55-1 and siTRIM55-2 significantly inhibited TNF-α and CCL2 expression at both the mRNA and protein levels in MCs. h The rescue assay showed that DBP-mediated CCL2 upregulation was blocked by si-TRIM55-1. si-TRIM55-1 and si-TRIM55-2, two siRNAs targeting TRIM55; Lv-Flag, lentivirus control; Lv-Flag-DBP: DBP-expressing lentivirus. *p < 0.05, **p < 0.01; n = 3. All assays were repeated at least three times
Techniques Used: Expressing, ChIP-sequencing, Luciferase, Rescue Assay, Control
Related Articles
Expressing:Article Title: The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis Article Snippet: Cells were crosslinked with 1% (v/v) formaldehyde for 10 min, and nuclear DNA was isolated using an EZ-CHIP-KIT (Millipore, Billerica, MA, USA) and sheared by sonication employing a cell disruptor (Sonics VCX150, Sonics and Materials Inc., Newtown, CT, USA). Control:Article Title: The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis Article Snippet: Cells were crosslinked with 1% (v/v) formaldehyde for 10 min, and nuclear DNA was isolated using an EZ-CHIP-KIT (Millipore, Billerica, MA, USA) and sheared by sonication employing a cell disruptor (Sonics VCX150, Sonics and Materials Inc., Newtown, CT, USA). ChIP-sequencing:Article Title: The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis Article Snippet: Cells were crosslinked with 1% (v/v) formaldehyde for 10 min, and nuclear DNA was isolated using an EZ-CHIP-KIT (Millipore, Billerica, MA, USA) and sheared by sonication employing a cell disruptor (Sonics VCX150, Sonics and Materials Inc., Newtown, CT, USA). Luciferase:Article Title: The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis Article Snippet: Cells were crosslinked with 1% (v/v) formaldehyde for 10 min, and nuclear DNA was isolated using an EZ-CHIP-KIT (Millipore, Billerica, MA, USA) and sheared by sonication employing a cell disruptor (Sonics VCX150, Sonics and Materials Inc., Newtown, CT, USA). Rescue Assay:Article Title: The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis Article Snippet: Cells were crosslinked with 1% (v/v) formaldehyde for 10 min, and nuclear DNA was isolated using an EZ-CHIP-KIT (Millipore, Billerica, MA, USA) and sheared by sonication employing a cell disruptor (Sonics VCX150, Sonics and Materials Inc., Newtown, CT, USA). |