transmission electron microscopy fixative (Servicebio Inc)
86
Structured Review
Servicebio Inc
transmission electron microscopy fixative
Transmission Electron Microscopy Fixative, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixed/electron+fixative+microscopy/pmc13264258-296-15-20
Average 86 stars, based on 1 article reviews
Transmission Electron Microscopy Fixative, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixed/electron+fixative+microscopy/pmc13264258-296-15-20
Average 86 stars, based on 1 article reviews
transmission electron microscopy fixative - by Bioz Stars,
2026-11
86/100 stars
Images
1) Product Images from "Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction"
Article Title: Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction
Journal: iScience
doi: 10.1016/j.isci.2026.116268
Figure Legend Snippet: Structural evaluation of the mouse TM following acute IOP elevation (A) Photographs reveal that mice underwent direct cannulation of the anterior chamber (AC) in the eye. Eyeballs were harvested immediately after the procedure, and the gross appearance is illustrated in the right. (B) Intraocular pressure (IOP) measurement following acute ocular hypertension. IOP was measured at baseline (0 days), 3 days, and 7 days following 45 mmHg pressure for 1 h. Data represent mean ± SD. two-way ANOVA was used for comparing IOP between control and HIOP groups across time points. Post hoc test: ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. control group at corresponding time point. N = 9 mice per group. (C) Representative hematoxylin staining of anterior chamber angles 3 days after IOP elevation. Magnification bars: 1 mm. (D) Ultrastructural appearance of the TM tissues by transmission electron microscopy. Leftmost: representative lower magnification image of the TM and SC. Right: higher magnification of the TM cells. The regions encased in black boxes are magnified. TM, trabecular meshwork; SC, Schlemm’s canal; red arrow indicates giant vacuoles.
Techniques Used: Control, Staining, Transmission Assay, Electron Microscopy
Figure Legend Snippet: Pathological changes in HTM cells under high hydrostatic pressure (A and B) Representative examples of human trabecular meshwork (HTM) cells cultured under control (con) or high hydrostatic pressure for 12 h, 1 day (1 D), and 3 days (3 D) immunolabeled for EDU (A) and TUNEL (B) assay. Scale bars, 50 μm. Bar graphs summarizing the effects of high hydrostatic pressure on the proliferation and death of HTM cells. N = 3. Magnification bars, 100 μm. (C) Histological evaluation of different groups of HTM cells by transmission electron microscopy. N = 3. Magnification bars, 50, 10, and 2 μm from the left to the right. (D) Representative fluorescence micrographs of F-actin in HTMs subjected to 12 h, 1D, and 3D culture under high hydrostatic pressure and the control, with nuclei and F-actin labeling shown in blue and green, respectively. The asterisk indicates cross-linked actin network (CLAN+) cells and the percentage of CLAN+ cells was analyzed. N = 5. Magnification bars, 25 μm. (E) Expression of fibronectin, collagen I and α-SMA in the different groups of HTM cells by western blots. N = 6. ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. Data represent mean ± SD.
Techniques Used: Cell Culture, Control, Immunolabeling, TUNEL Assay, Transmission Assay, Electron Microscopy, Fluorescence, Labeling, Expressing, Western Blot
Related Articles
Centrifugation:Article Title: Multi-Targeting Carnosic Acid Kills Drug-Resistant Helicobacter pylori With Narrow-Spectrum Activity. Article Snippet: .. Briefly, H. pylori G27 (1 × 108 CFU mL− 1 ) was treated with DMSO, CA (1 × and 2 ×MIC), or PMB (1 × and 2 ×MIC) for 1 h. Cells were gently washed with PBS, collected by centrifugation, and fixed with Electron Microscopy:Article Title: Multi-Targeting Carnosic Acid Kills Drug-Resistant Helicobacter pylori With Narrow-Spectrum Activity. Article Snippet: .. Briefly, H. pylori G27 (1 × 108 CFU mL− 1 ) was treated with DMSO, CA (1 × and 2 ×MIC), or PMB (1 × and 2 ×MIC) for 1 h. Cells were gently washed with PBS, collected by centrifugation, and fixed with Article Title: Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction Article Snippet: IOP was measured in a masked manner using a handheld tonometer (Tonolab; Icare Finland Oy, Vantaa, Finland), with each measurement repeated five times. .. Following dissection, the TM or HTM cells grown on coverslips were immediately immersed in fresh Article Title: The cysteine metabolic regulation through CSE-transsulfuration pathway is essential in iron-induced oxidative damage of heart. Article Snippet: .. The supernatant was carefully aspirated, and 1 mL of Article Title: HMGB1-mediated endoplasmic reticulum stress in monocyte-derived dendritic cells regulates CD4+ T cell immune responses in myasthenia gravis. Article Snippet: The data obtained were analyzed using BGI Dr. Tom online system (https://biosys.bgi.com). .. The cells were fixed with Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia Article Snippet: .. Immediately after euthanasia, lung tissues were harvested and fixed with Microscopy:Article Title: Green tea catechin EGCG attenuates hippocampal atrophy and cognitive impairment in obesity via autophagy signaling. Article Snippet: .. These blocks were additionally fixed in fresh Article Title: Kaempferol inhibits oxidative stress-induced ferroptosis via the ROS/P38MAPK pathway to delay intervertebral disc degeneration: a combinatorial study of cell, animal, and transcriptomic evidence. Article Snippet: Intervertebral disc degeneration (IVDD) is a well-known major contributor to lower back pain and has become a significant challenge in the field of public health.. Kaempferol (KAE) is a naturally occurring flavonoid compound found in various herbs and plant families, known for its potent anti-inflammatory and antioxidant pharmacological effects.. Ferroptosis is a recently discovered form of cell death characterized by iron dependence and the uncontrollable accumulation of lipid peroxides. Article Title: Green tea catechin EGCG attenuates hippocampal atrophy and cognitive impairment in obesity via autophagy signaling. Article Snippet: .. For electron microscopic analysis, mice were deeply anesthetized with sodium pentobarbital (i.p.) and transcardially perfused with icecold saline, followed by Dissection:Article Title: Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction Article Snippet: IOP was measured in a masked manner using a handheld tonometer (Tonolab; Icare Finland Oy, Vantaa, Finland), with each measurement repeated five times. .. Following dissection, the TM or HTM cells grown on coverslips were immediately immersed in fresh Transmission Assay:Article Title: Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction Article Snippet: IOP was measured in a masked manner using a handheld tonometer (Tonolab; Icare Finland Oy, Vantaa, Finland), with each measurement repeated five times. .. Following dissection, the TM or HTM cells grown on coverslips were immediately immersed in fresh Saline:Article Title: Green tea catechin EGCG attenuates hippocampal atrophy and cognitive impairment in obesity via autophagy signaling. Article Snippet: .. For electron microscopic analysis, mice were deeply anesthetized with sodium pentobarbital (i.p.) and transcardially perfused with icecold saline, followed by |
