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paraformaldehyde fixative solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher paraformaldehyde fixative solution
    Paraformaldehyde Fixative Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixative+solution/Paraformaldehyde+fixative+solution/pm41929240-289-14-18
    Average 97 stars, based on 1 article reviews
    paraformaldehyde fixative solution - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Saline:

    Article Title: Preclinical Evaluation of [ 18 F]JNJ-1: A Novel Positron Emission Tomography Ligand Targeting AMPAR/TARP γ8.
    Article Snippet: Purpose The AMPA receptor (AMPARs) mediates fast excitatory neurotransmission and is the central to synaptic plasticity, learning and memory dysregulation of this system has been implicated in epilepsy, neurodegeneration, and neuropsychiatric disorders.. The transmembrane AMPA receptor regulatory protein γ8 (TARP γ8) plays a crucial role in the expression and localization of AMPARs within the brain, particularly in the hippocampus and cortex.. A selective positron emission tomography (PET) ligand to image this target is of significant interest for investigating potential clinical AMPAR/TARP γ8 antagonists.

    Incubation:

    Article Title: Preclinical Evaluation of [ 18 F]JNJ-1: A Novel Positron Emission Tomography Ligand Targeting AMPAR/TARP γ8.
    Article Snippet: Purpose The AMPA receptor (AMPARs) mediates fast excitatory neurotransmission and is the central to synaptic plasticity, learning and memory dysregulation of this system has been implicated in epilepsy, neurodegeneration, and neuropsychiatric disorders.. The transmembrane AMPA receptor regulatory protein γ8 (TARP γ8) plays a crucial role in the expression and localization of AMPARs within the brain, particularly in the hippocampus and cortex.. A selective positron emission tomography (PET) ligand to image this target is of significant interest for investigating potential clinical AMPAR/TARP γ8 antagonists.

    Concentration Assay:

    Article Title: Preclinical Evaluation of [ 18 F]JNJ-1: A Novel Positron Emission Tomography Ligand Targeting AMPAR/TARP γ8.
    Article Snippet: Purpose The AMPA receptor (AMPARs) mediates fast excitatory neurotransmission and is the central to synaptic plasticity, learning and memory dysregulation of this system has been implicated in epilepsy, neurodegeneration, and neuropsychiatric disorders.. The transmembrane AMPA receptor regulatory protein γ8 (TARP γ8) plays a crucial role in the expression and localization of AMPARs within the brain, particularly in the hippocampus and cortex.. A selective positron emission tomography (PET) ligand to image this target is of significant interest for investigating potential clinical AMPAR/TARP γ8 antagonists.

    Microscopy:

    Article Title: Eukaryotic tRNA ligases mediate RNA break repair
    Article Snippet: .. After 24 h, cells were washed with 1x PBS (Gibco), fixed in 4% paraformaldehyde (Thermo Scientific) in 1x PBS, then washed three times with 1x PBS and imaged by widefield microscopy using 20x magnification (Eclipse Ti2 series, Nikon). ..

    ATP Assay:

    Article Title: Peroxiredoxin Ⅲ safeguards cardiac function against doxorubicin by regulating mitochondrial quality control via H 2 O 2 detoxification
    Article Snippet: .. DOX was kindly provided from Donga-ST Co., Ltd. (Seoul, Republic of Korea); Fetal Bovine Serum (FBS) and antibiotic-antimycotic (A/A) were from Gibco (NY, USA); Dimethyl sulfoxide (DMSO), poly- l -lysine, puromycin, and bafilomycin A1 (BafA1) were from Sigma-Aldrich (St. Louis, MO, USA); 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt (WST-1) reagent was purchased from Roche Applied Science (Basel, Switzerland); Lipofectamine 2000, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA), Rhodamine-123 (Rho-123), Mitotracker Red CMX, Lysosensor Green DND-189, DQ-BSA and 7-Aminoactinomycin D (7-AAD) were purchased from Invitrogen (CA, USA); 10-N-nonylacridine orange (10-NAO) was purchased from Molecular Probes (Eugene, OR, USA); Mitochondria peroxy yellow-1 (MitoPY-1) was from Tocris Biosciences (Bristol, UK); Magic Red kit was from ImmunoChemistry Technologies (Davis, CA, USA); paraformaldehyde (PFA), 4′,6-diamidno-2-phenylindole (DAPI) were from Thermo Fisher Scientific (Waltham, MA, USA); Adenosine triphosphate (ATP) and cell viability ATP assay kit was from Promega (Madison, WI, USA); Seahorse XF cell Mitostress test kit, Seahorse XF DMEM Medium, and Seahorse XF Calibrant Solution was from Agilent (Santa Clara, CA, USA); Elamipretide (SS-31) was from Biorbyt Ltd. (Cambridge, UK); Mitoquinone (MitoQ) and Visomitin (SKQ1) were from MedChemExpress (Monmouth junction, NJ, USA); Mouse Troponin I ELISA kit was from Abcam (Cambridge, UK). .. The primary antibodies against poly(ADP-ribose) polymerase (PARP), cleaved caspase-3, pDrp1 (Ser616), light-chain 3B (LC3B), SQSTM1/p62, thioredoxin 2 (Trx2), lysosome-associated membrane protein 1 (LAMP1), nuclear respiratory factor (Nrf1), forkhead box O3a (FOXO3a) were purchased from Cell Signaling Technology (Danvers, MA, USA); Parkin, Bcl2 adenovirus e1B 19 kDa interacting protein 3 (BNIP3), Drp1, Bax, peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), Mfn1 and Mfn2 were purchased from Santa Cruz Biotechnologies (Dallas, TX, USA); PrxV, OPA1, PTEN-induced kinase 1 (PINK1) and mitochondria transcription factor (mtTFA) were purchased from Abcam (Cambridge, UK); Tomm20 was from Sigma-Aldrich (St. Louis, MO, USA); catalase, glutathione peroxidase IV (GpxIV), superoxide dismutase 2 (SOD2), α-tubulin, β-actin and PrxIII (mono) were purchased from Ab Frontier (Seoul, Republic of Korea); glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was purchased from Chemicon (Jincheon, Republic of Korea); The secondary antibodies against anti-mouse IgG HRP and anti-rabbit IgG HRP were purchased from Seracare (Milford, MA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Peroxiredoxin Ⅲ safeguards cardiac function against doxorubicin by regulating mitochondrial quality control via H 2 O 2 detoxification
    Article Snippet: .. DOX was kindly provided from Donga-ST Co., Ltd. (Seoul, Republic of Korea); Fetal Bovine Serum (FBS) and antibiotic-antimycotic (A/A) were from Gibco (NY, USA); Dimethyl sulfoxide (DMSO), poly- l -lysine, puromycin, and bafilomycin A1 (BafA1) were from Sigma-Aldrich (St. Louis, MO, USA); 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt (WST-1) reagent was purchased from Roche Applied Science (Basel, Switzerland); Lipofectamine 2000, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA), Rhodamine-123 (Rho-123), Mitotracker Red CMX, Lysosensor Green DND-189, DQ-BSA and 7-Aminoactinomycin D (7-AAD) were purchased from Invitrogen (CA, USA); 10-N-nonylacridine orange (10-NAO) was purchased from Molecular Probes (Eugene, OR, USA); Mitochondria peroxy yellow-1 (MitoPY-1) was from Tocris Biosciences (Bristol, UK); Magic Red kit was from ImmunoChemistry Technologies (Davis, CA, USA); paraformaldehyde (PFA), 4′,6-diamidno-2-phenylindole (DAPI) were from Thermo Fisher Scientific (Waltham, MA, USA); Adenosine triphosphate (ATP) and cell viability ATP assay kit was from Promega (Madison, WI, USA); Seahorse XF cell Mitostress test kit, Seahorse XF DMEM Medium, and Seahorse XF Calibrant Solution was from Agilent (Santa Clara, CA, USA); Elamipretide (SS-31) was from Biorbyt Ltd. (Cambridge, UK); Mitoquinone (MitoQ) and Visomitin (SKQ1) were from MedChemExpress (Monmouth junction, NJ, USA); Mouse Troponin I ELISA kit was from Abcam (Cambridge, UK). .. The primary antibodies against poly(ADP-ribose) polymerase (PARP), cleaved caspase-3, pDrp1 (Ser616), light-chain 3B (LC3B), SQSTM1/p62, thioredoxin 2 (Trx2), lysosome-associated membrane protein 1 (LAMP1), nuclear respiratory factor (Nrf1), forkhead box O3a (FOXO3a) were purchased from Cell Signaling Technology (Danvers, MA, USA); Parkin, Bcl2 adenovirus e1B 19 kDa interacting protein 3 (BNIP3), Drp1, Bax, peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), Mfn1 and Mfn2 were purchased from Santa Cruz Biotechnologies (Dallas, TX, USA); PrxV, OPA1, PTEN-induced kinase 1 (PINK1) and mitochondria transcription factor (mtTFA) were purchased from Abcam (Cambridge, UK); Tomm20 was from Sigma-Aldrich (St. Louis, MO, USA); catalase, glutathione peroxidase IV (GpxIV), superoxide dismutase 2 (SOD2), α-tubulin, β-actin and PrxIII (mono) were purchased from Ab Frontier (Seoul, Republic of Korea); glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was purchased from Chemicon (Jincheon, Republic of Korea); The secondary antibodies against anti-mouse IgG HRP and anti-rabbit IgG HRP were purchased from Seracare (Milford, MA, USA).



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