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Becton Dickinson lyse-fix buffer
Lyse Fix Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixative+solution/fixing+lysing+solution/pm37894453-51-14-16
Average 90 stars, based on 1 article reviews
lyse-fix buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

FACS:

Article Title: Preclinical characterization of the Toll-like receptor 7/8 antagonist MHV370 for lupus therapy
Article Snippet: Cells were incubated with pre-warmed Lyse-Fix Buffer (BD) for 15 min at 37°C, washed in FACS buffer (PBS w/o Ca 2+ /Mg 2+ /2% FBS/5 mM EDTA) and analyzed using an LSRII flow cytometer (BD Biosciences).

Article Title: Efficiency of nilotinib to target chronic phase-chronic myeloid leukaemia primary mature CD34 - and immature CD34 + cells.
Article Snippet: Briefly, 1 × 106 cells were fixed using BD Fix and Lyse buffer, and then permeabilized with BD Phosflow Perm Buffer III (Becton Dickinson, Le Pont de Claix, France).

Article Title: Spontaneous Neutrophil Extracellular Traps Release Are Inflammatory Markers Associated with Hyperglycemia and Renal Failure on Diabetic Retinopathy.
Article Snippet: Afterward, 0.9 mL 1X of lysing and fixing solutions (BD FACS Lysing) were added to samples and incubated for 15 min at room temperature (RT) in darkness.

Article Title: Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin
Article Snippet: Cells were then washed and fixed using Lyse Fix buffer (BD Biosciences).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 °C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Interferon receptor gene dosage determines diverse hallmarks of Down syndrome
Article Snippet: Following stimulation, cells were washed in FACS buffer, subjected to BD Lyse-Fix buffer (BD Biosciences, Cat# 5580249), then to permeabilization buffer III (BD Biosciences, Cat# 558050).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance.
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 ◦C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Endosome dysfunction leads to gain-of-function TLR7 and human lupus
Article Snippet: For immune phenotyping, whole blood was antibody stained in brilliant stain Buffer (Becton Dickinson (BD), San Jose, USA) at ambient temperature, lysed with BD Lyse-fix buffer, washed 2 times and live cells were acquired on a BD LSR Fortessa cytometer with FACS Diva software.

Incubation:

Article Title: Preclinical characterization of the Toll-like receptor 7/8 antagonist MHV370 for lupus therapy
Article Snippet: Cells were incubated with pre-warmed Lyse-Fix Buffer (BD) for 15 min at 37°C, washed in FACS buffer (PBS w/o Ca 2+ /Mg 2+ /2% FBS/5 mM EDTA) and analyzed using an LSRII flow cytometer (BD Biosciences).

Article Title: Efficiency of nilotinib to target chronic phase-chronic myeloid leukaemia primary mature CD34 - and immature CD34 + cells.
Article Snippet: Briefly, 1 × 106 cells were fixed using BD Fix and Lyse buffer, and then permeabilized with BD Phosflow Perm Buffer III (Becton Dickinson, Le Pont de Claix, France).

Article Title: Spontaneous Neutrophil Extracellular Traps Release Are Inflammatory Markers Associated with Hyperglycemia and Renal Failure on Diabetic Retinopathy.
Article Snippet: Afterward, 0.9 mL 1X of lysing and fixing solutions (BD FACS Lysing) were added to samples and incubated for 15 min at room temperature (RT) in darkness.

Article Title: Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin
Article Snippet: Cells were then washed and fixed using Lyse Fix buffer (BD Biosciences).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 °C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Interferon receptor gene dosage determines diverse hallmarks of Down syndrome
Article Snippet: Following stimulation, cells were washed in FACS buffer, subjected to BD Lyse-Fix buffer (BD Biosciences, Cat# 5580249), then to permeabilization buffer III (BD Biosciences, Cat# 558050).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance.
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 ◦C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Endosome dysfunction leads to gain-of-function TLR7 and human lupus
Article Snippet: For immune phenotyping, whole blood was antibody stained in brilliant stain Buffer (Becton Dickinson (BD), San Jose, USA) at ambient temperature, lysed with BD Lyse-fix buffer, washed 2 times and live cells were acquired on a BD LSR Fortessa cytometer with FACS Diva software.

Flow Cytometry:

Article Title: Preclinical characterization of the Toll-like receptor 7/8 antagonist MHV370 for lupus therapy
Article Snippet: Cells were incubated with pre-warmed Lyse-Fix Buffer (BD) for 15 min at 37°C, washed in FACS buffer (PBS w/o Ca 2+ /Mg 2+ /2% FBS/5 mM EDTA) and analyzed using an LSRII flow cytometer (BD Biosciences).

Article Title: Efficiency of nilotinib to target chronic phase-chronic myeloid leukaemia primary mature CD34 - and immature CD34 + cells.
Article Snippet: Briefly, 1 × 106 cells were fixed using BD Fix and Lyse buffer, and then permeabilized with BD Phosflow Perm Buffer III (Becton Dickinson, Le Pont de Claix, France).

Article Title: Spontaneous Neutrophil Extracellular Traps Release Are Inflammatory Markers Associated with Hyperglycemia and Renal Failure on Diabetic Retinopathy.
Article Snippet: Afterward, 0.9 mL 1X of lysing and fixing solutions (BD FACS Lysing) were added to samples and incubated for 15 min at room temperature (RT) in darkness.

Article Title: Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin
Article Snippet: Cells were then washed and fixed using Lyse Fix buffer (BD Biosciences).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 °C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Interferon receptor gene dosage determines diverse hallmarks of Down syndrome
Article Snippet: Following stimulation, cells were washed in FACS buffer, subjected to BD Lyse-Fix buffer (BD Biosciences, Cat# 5580249), then to permeabilization buffer III (BD Biosciences, Cat# 558050).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance.
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 ◦C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Endosome dysfunction leads to gain-of-function TLR7 and human lupus
Article Snippet: For immune phenotyping, whole blood was antibody stained in brilliant stain Buffer (Becton Dickinson (BD), San Jose, USA) at ambient temperature, lysed with BD Lyse-fix buffer, washed 2 times and live cells were acquired on a BD LSR Fortessa cytometer with FACS Diva software.

Staining:

Article Title: Preclinical characterization of the Toll-like receptor 7/8 antagonist MHV370 for lupus therapy
Article Snippet: Cells were incubated with pre-warmed Lyse-Fix Buffer (BD) for 15 min at 37°C, washed in FACS buffer (PBS w/o Ca 2+ /Mg 2+ /2% FBS/5 mM EDTA) and analyzed using an LSRII flow cytometer (BD Biosciences).

Article Title: Efficiency of nilotinib to target chronic phase-chronic myeloid leukaemia primary mature CD34 - and immature CD34 + cells.
Article Snippet: Briefly, 1 × 106 cells were fixed using BD Fix and Lyse buffer, and then permeabilized with BD Phosflow Perm Buffer III (Becton Dickinson, Le Pont de Claix, France).

Article Title: Spontaneous Neutrophil Extracellular Traps Release Are Inflammatory Markers Associated with Hyperglycemia and Renal Failure on Diabetic Retinopathy.
Article Snippet: Afterward, 0.9 mL 1X of lysing and fixing solutions (BD FACS Lysing) were added to samples and incubated for 15 min at room temperature (RT) in darkness.

Article Title: Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin
Article Snippet: Cells were then washed and fixed using Lyse Fix buffer (BD Biosciences).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 °C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Interferon receptor gene dosage determines diverse hallmarks of Down syndrome
Article Snippet: Following stimulation, cells were washed in FACS buffer, subjected to BD Lyse-Fix buffer (BD Biosciences, Cat# 5580249), then to permeabilization buffer III (BD Biosciences, Cat# 558050).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance.
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 ◦C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Endosome dysfunction leads to gain-of-function TLR7 and human lupus
Article Snippet: For immune phenotyping, whole blood was antibody stained in brilliant stain Buffer (Becton Dickinson (BD), San Jose, USA) at ambient temperature, lysed with BD Lyse-fix buffer, washed 2 times and live cells were acquired on a BD LSR Fortessa cytometer with FACS Diva software.

Cytometry:

Article Title: Preclinical characterization of the Toll-like receptor 7/8 antagonist MHV370 for lupus therapy
Article Snippet: Cells were incubated with pre-warmed Lyse-Fix Buffer (BD) for 15 min at 37°C, washed in FACS buffer (PBS w/o Ca 2+ /Mg 2+ /2% FBS/5 mM EDTA) and analyzed using an LSRII flow cytometer (BD Biosciences).

Article Title: Efficiency of nilotinib to target chronic phase-chronic myeloid leukaemia primary mature CD34 - and immature CD34 + cells.
Article Snippet: Briefly, 1 × 106 cells were fixed using BD Fix and Lyse buffer, and then permeabilized with BD Phosflow Perm Buffer III (Becton Dickinson, Le Pont de Claix, France).

Article Title: Spontaneous Neutrophil Extracellular Traps Release Are Inflammatory Markers Associated with Hyperglycemia and Renal Failure on Diabetic Retinopathy.
Article Snippet: Afterward, 0.9 mL 1X of lysing and fixing solutions (BD FACS Lysing) were added to samples and incubated for 15 min at room temperature (RT) in darkness.

Article Title: Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin
Article Snippet: Cells were then washed and fixed using Lyse Fix buffer (BD Biosciences).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 °C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Interferon receptor gene dosage determines diverse hallmarks of Down syndrome
Article Snippet: Following stimulation, cells were washed in FACS buffer, subjected to BD Lyse-Fix buffer (BD Biosciences, Cat# 5580249), then to permeabilization buffer III (BD Biosciences, Cat# 558050).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance.
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 ◦C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Endosome dysfunction leads to gain-of-function TLR7 and human lupus
Article Snippet: For immune phenotyping, whole blood was antibody stained in brilliant stain Buffer (Becton Dickinson (BD), San Jose, USA) at ambient temperature, lysed with BD Lyse-fix buffer, washed 2 times and live cells were acquired on a BD LSR Fortessa cytometer with FACS Diva software.

Software:

Article Title: Preclinical characterization of the Toll-like receptor 7/8 antagonist MHV370 for lupus therapy
Article Snippet: Cells were incubated with pre-warmed Lyse-Fix Buffer (BD) for 15 min at 37°C, washed in FACS buffer (PBS w/o Ca 2+ /Mg 2+ /2% FBS/5 mM EDTA) and analyzed using an LSRII flow cytometer (BD Biosciences).

Article Title: Efficiency of nilotinib to target chronic phase-chronic myeloid leukaemia primary mature CD34 - and immature CD34 + cells.
Article Snippet: Briefly, 1 × 106 cells were fixed using BD Fix and Lyse buffer, and then permeabilized with BD Phosflow Perm Buffer III (Becton Dickinson, Le Pont de Claix, France).

Article Title: Spontaneous Neutrophil Extracellular Traps Release Are Inflammatory Markers Associated with Hyperglycemia and Renal Failure on Diabetic Retinopathy.
Article Snippet: Afterward, 0.9 mL 1X of lysing and fixing solutions (BD FACS Lysing) were added to samples and incubated for 15 min at room temperature (RT) in darkness.

Article Title: Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin
Article Snippet: Cells were then washed and fixed using Lyse Fix buffer (BD Biosciences).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 °C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Interferon receptor gene dosage determines diverse hallmarks of Down syndrome
Article Snippet: Following stimulation, cells were washed in FACS buffer, subjected to BD Lyse-Fix buffer (BD Biosciences, Cat# 5580249), then to permeabilization buffer III (BD Biosciences, Cat# 558050).

Article Title: MicroRNA Expression Patterns Reveal a Role of the TGF-β Family Signaling in AML Chemo-Resistance.
Article Snippet: For intracellular staining of phosphorylated SMAD (pSMAD), cells were fixed in 1 mL of Lyse-Fix buffer (BD Bioscience, San Jose, CA, USA) for 10 min at 37 ◦C and then permeabilized on ice in permbuffer (BD Bioscience) for 30 min.

Article Title: Endosome dysfunction leads to gain-of-function TLR7 and human lupus
Article Snippet: For immune phenotyping, whole blood was antibody stained in brilliant stain Buffer (Becton Dickinson (BD), San Jose, USA) at ambient temperature, lysed with BD Lyse-fix buffer, washed 2 times and live cells were acquired on a BD LSR Fortessa cytometer with FACS Diva software.



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