Blocking Assay:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Staining:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Article Title: Bilirubin directly activates RIPK3 to induce non-classical necroptosis.
Article Snippet: Immunofluorescence images were acquired using a confocal laser scanning microscope (ZEISS LSM 980). .. Immunofluorescence staining of frozen tissue sections The frozen slices were equilibrated at 37 °C for 20min, fixed with fixation solution (Servicebio G1101) for 30min, and then washed three times with PBS. .. The sections were subsequently blocked with a sealing solution containing 0.3% Triton (Solarbio, T8200) and 5% BSA (Sigma‒Aldrich, 9048- 46-8) for 70min, followed by three washes with PBS.
Electron Microscopy:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Cell Isolation:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Magnetic Cell Separation:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Single Cell:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Real-time Polymerase Chain Reaction:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
SYBR Green Assay:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Enzyme-linked Immunosorbent Assay:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Western Blot:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Membrane:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Cell Culture:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Software:Article Title: Natural killer cell dysfunction drives keloid pathogenesis.
Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081059 ExoSAP-IT Affymetrix Cat# 78200 SuperScriptTM III Reverse Transcriptase Invitrogen Cat# 18080-093 Fixable Viability Dye efluor 780 Invitrogen Cat# 65-0865-18 MitoTrackerTM Red CMXRos for Flow Cytometry Invitrogen Cat# M46752 MitoSOXTM Green Mitochondrial Superoxide Indicator Invitrogen Cat# M36005 RIPA Lysis Buffer Beyotime Cat# P0013B Protease and phosphatase inhibitor cocktail Beyotime Cat# P1045 Tween 20 Beyotime Cat# ST825-100mL SDS-PAGE Loading Buffer Beyotime Cat# P0015L PVDF Membrane Merck Millipore Cat# IPVH00010 Molecular Weight Marker Beyotime Cat# P0071 BiofurawTM MOPS Running Buffer Tanon Cat# BT8100-2002 Precast Gel for SDS-PAGE Tanon Cat# 180-9110H Western Transfer Buffer Beyotime Cat# P0021B EZ-Buffers H 10 x TBST Buffer Sangon Biotech Cat# C520009-0001 Non-fat powdered milk Beyotime Cat#P0216-300g Hematoxylin-eosin HD Servicebio Cat#G1076 Tris-EDTA antigen retrieval solution Servicebio Cat# G1203-250mL 4% paraformaldehyde Biosharp Cat# BL539A (Continued on next page) 18 Cell Reports 45, 117129, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biodewax and Clear Solution Servicebio Cat# G1128 Peroxidase blocking solution Absin Cat# abs9333 Triton X-100 Servicebio Cat# GC204003-100mL Antifade Mounting Medium Beyotime Cat# P0126-25mL DAPI Staining Solution Beyotime Cat# C1005 Electron microscopy fixation solution Servicebio Cat# G1102-100mL Lipofectamine RNAiMAX Reagent Invitrogen Cat# 13778-100 Critical commercial assays RNA fragmentation reagents Invitrogen Cat# AM8740 P3 Primary Cell 4D-NucleofectorTM X Kit S Lonza Cat# V4XP-3032 EasySep Human NK Cell Isolation Kit STEMCELL Cat# 17955 NK MACS Medium human MACS Cat# 130-114-429 SeekOne® DD Single Cell 5′ library preparation kit SeekGene Cat# K00501 NEBNext Ultra II Non directional Second Strand Synthesis Module NEB Cat# E6111L HiScribe T7 High Yield RNA Synthesis Kit NEB Cat# E2040S HiScript III All-in-one RT SuperMix Perfect for qPCR Vazyme Cat# R333 SYBR Green Premix Pro Taq HS qPCR Kit AGbio Cat# AG11718 Human TGF-β1 Elisa Kit MULTI SCIENCES Cat# EK981 Human Lipopolysaccharides Elisa Kit CUSABIO Cat# CSB-E09945h Human IFN-β Elisa Kit Elabscience Cat# E-EL-H0085 BCA Protein Quantification Kit Beyotime Cat# P0012S Western Luminescent Detection Kit Vigorous Biotechnology Cat# P004 Seahorse XF Cell Mitochondrial Stress Test Kit Agilent Cat# 103015-100 Seahorse XF Glycolytic Rate Assay Kit Agilent Cat# 103344-100 Masson Staining Kit Servicebio Cat# G1006 JC-1 Mitochondrial Membrane Potential Assay Kit MCE Cat# HY-K0601-100T Deposited data ScRNA-Seq: NK cells from healthy donor and people with keloids This paper GSA: HRA008790 Bulk RNA-Seq: keloid fibroblasts cultured in IFNγ+AREG– vs. IFN-γ+AREG+ conditioned medium This paper GSA: HRA008880 Bulk RNA-Seq: NK cells cultured with or without IFN-β for 7 days This paper GSA: HRA008788 Software and algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ GraphPad Prism GraphPad Software, Inc https://www.graphpad.com/scientific-software/ prism/ SAMtools Li et al.62 http://www.htslib.org R Statistical Computing Software The R Foundation https://www.r-project.org/ DESeq2 Love et al.63 https://bioconductor.org/packages/release/bioc/ html/DESeq2.htmll Seurat 3.0 Stuart et al.64 http://satijalab.org/seurat/ Bowtie2 Langmead et al.65 https://bowtie-bio.sourceforge.net/bowtie2/index. shtml CEL-Seq2 Hashimshony et al.66 https://github.com/yanailab/celseq2 Cell Reports 45, 117129, April 28, 2026 19 ..
Transmission Electron Microscopy:
Immunofluorescence:Article Title: Bilirubin directly activates RIPK3 to induce non-classical necroptosis.
Article Snippet: Immunofluorescence images were acquired using a confocal laser scanning microscope (ZEISS LSM 980). .. Immunofluorescence staining of frozen tissue sections The frozen slices were equilibrated at 37 °C for 20min, fixed with fixation solution (Servicebio G1101) for 30min, and then washed three times with PBS. .. The sections were subsequently blocked with a sealing solution containing 0.3% Triton (Solarbio, T8200) and 5% BSA (Sigma‒Aldrich, 9048- 46-8) for 70min, followed by three washes with PBS.
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