intracellular fixation permeabilization buffer kit (Elabscience Biotechnology)

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Elabscience Biotechnology intracellular fixation permeabilization buffer kit
Intracellular Fixation Permeabilization Buffer Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixation+permeabilization+buffer/Intracellular+Fixation%2FPermeabilization+Buffer+Kit/pm41514495-314-6-11
Average 95 stars, based on 72 article reviews

intracellular fixation permeabilization buffer kit - by Bioz Stars, 2026-10

95/100 stars

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Purification:

Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer
Article Snippet: Imiquimod (IMQ) was purchased from TCI (Tokyo, Japan). .. Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China). .. IL-4 was purchased from Biyuntian Biotechnology (Shanghai, China).

Enzyme-linked Immunosorbent Assay:

Staining:

Article Title: Identification of Active Compounds of Centipeda minima for Potentiating Tumor Immunotherapy in Non-Small Cell Lung Cancer via FANCD2-cGAS/STING/IFN-I Signaling Axis.
Article Snippet: .. For the intracellular factor IFNγ staining, the cells were firstly permeabilized by using the Intracellular Fixation/Permeabilization Kit (Elabscience). .. Flow cytometry was performed and analyzed using a CytoFLEX- S flow cytometer along with CytExpert software (Beckman Coulter, California, USA).

Article Title: Suppression of Nr1d1/Bnip3-dependent mitophagy by notch signaling aggravates inflammatory response in RSV-infected mice lungs.
Article Snippet: 1 Department of Pediatrics, Women and Children’s Hospital, Zhongnan Hospital of Wuhan University, 169# Donghu Road, Wuhan 430062, Hubei, China 2 Jingmen Central Hospital, Central Hospital affiliated to Jingchu University of Technology, Jingmen, China 3 Children’s Digital Health and Data Center, Wuhan University, Wuhan 430062, Hubei, China Abstract Background Alveolar macrophages (AMs) are crucial for defending against respiratory syncytial virus (RSV) and regulating lung inflammation.. Notch signaling is essential for macrophage polarization and its functional diversity.However, the role of macrophages Notch1 in RSV-induced lung injury and its underlying mechanisms remain incompletely understood.. Methods This study used macrophage-specific Notch1 knockout (MKO) mice to investigate the lung lesions and inflammation in RSV-infected mice.

Cell Stimulation:

Article Title: In depth study on the mechanism of Xiao-Chai-Hu-Tang in ameliorating irinotecan-induced intestinal toxicity and enhancing antitumor immunity via intestinal microbiota and fecal metabolomics.
Article Snippet: Irinotecan (CPT− 11) is a cornerstone chemotherapeutic for advanced colorectal cancer (CRC), but its clinical use is limited by dose-dependent gastrointestinal (GI) toxicity.. Xiao-Chai-Hu-Tang (XCHT), a classical traditional Chinese medicine formula, alleviates CPT− 11-induced adverse effects, yet its mechanism remains unclear.. This study established a CPT− 11-induced intestinal toxicity model in CRC rats, using multi-omics analyses (16S rRNA sequencing, fecal metabolomics, SCFA quantification) and an antibiotic depletion model to explore XCHT’s mechanism via the gut microbiota-metabolite axis.

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