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intracellular fixation permeabilization buffer kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology intracellular fixation permeabilization buffer kit
    Intracellular Fixation Permeabilization Buffer Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixation+permeabilization+buffer+kit/Intracellular+Fixation%2FPermeabilization+Buffer+Kit/pm41961300-97-38-42
    Average 95 stars, based on 67 article reviews
    intracellular fixation permeabilization buffer kit - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Biomimetic sequential immuno-osteogenic regulation via a dual-drug delivery system with PDA coating on sulfonated PEEK promotes bone defect repair
    Article Snippet: The following reagents were used in the experiments: PEEK (Nanjing First Plastic Special Engineering Plastic Products Co., Ltd., Nanjing, China), simulated body fluid, dexamethasone, β-glycerol phosphate, ascorbic acid and fluorescein isothiocyanate-labeled (FITC)-labeled phalloidin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China), 2-methylimidazole (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), Zinc nitrate hexahydrate (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), Recombinant Rat Interleukin-4 (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), cell counting Kit (CCK)-8, human IL-4R ELISA Kit (YOBIBIO (Shanghai) Biotechnology Co., Ltd., Shanghai, China), Calcein/PI Cell Viability/Cytotoxicity Assay Kit, BCIP/NBT Alkaline Phosphatase Color Development Kit, Alkaline Phosphatase Assay Kit, Alizarin Red S Staining Kit for Osteogenesis and DAPI Staining Solution (Beyotime Biotechnology Co., Ltd., Shanghai, China), Reverse transcription Kit and 2 × RealStar Fast SYBR qPCR Mix (GenStar Biosolutions Co., Ltd., Beijing, China), CD206 Polyclonal antibody (Cat No. 18704-1-AP), iNOS Polyclonal antibody (Cat No. 14142-1-AP), β-actin Polyclonal antibody (Cat No. 20536-1- AP), Osteopontin Polyclonal antibody (Cat No. 22952-1-AP), NF-κB p65 Recombinant antibody (Cat No. 82335-1-RR), Fluorescein (FITC)– conjugated goat anti-rabbit IgG (H + L) (Cat No. SA00003-2), Cy3–conjugated goat anti-rabbit IgG (H + L) (Cat No. SA00009-2) and HRP-conjugated goat anti-rabbit IgG (H + L) (Cat No. SA00001-2) (Proteintech Group, Inc., Wuhan, China), Recombinant Anti-Collagen I antibody (ab138492, Abcam Shanghai Trading Co., Ltd., Shanghai, China), IL-6 Polyclonal Antibody (YP-Ab-16022), IL-10 Polyclonal Antibody (YP-Ab-15986) and IKK alpha Rabbit Polyclonal Antibody (YP-mAb-14162) (UpingBio technology Co., Ltd., Hangzhou China), Bone Morphogenetic Protein 2 (BMP-2) Polyclonal antibody (#AF5163, Affinity Biosciences Group Ltd., Jiangsu, China), APC anti-mouseW CD86 antibody (0.2 μg/μL; #PO3, BioLegend, USA), PE anti-mouse CD206 antibody (0.2 μg/μL; #C068C2, BioLegend, USA), Intracellular Fixation/Permeabilization Buffer Kit (E-CK-A109, Elabscience, China).

    Purification:

    Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer
    Article Snippet: Imiquimod (IMQ) was purchased from TCI (Tokyo, Japan). .. Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China). .. IL-4 was purchased from Biyuntian Biotechnology (Shanghai, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer
    Article Snippet: Imiquimod (IMQ) was purchased from TCI (Tokyo, Japan). .. Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China). .. IL-4 was purchased from Biyuntian Biotechnology (Shanghai, China).

    Staining:

    Article Title: Suppression of Nr1d1/Bnip3-dependent mitophagy by notch signaling aggravates inflammatory response in RSV-infected mice lungs.
    Article Snippet: 1 Department of Pediatrics, Women and Children’s Hospital, Zhongnan Hospital of Wuhan University, 169# Donghu Road, Wuhan 430062, Hubei, China 2 Jingmen Central Hospital, Central Hospital affiliated to Jingchu University of Technology, Jingmen, China 3 Children’s Digital Health and Data Center, Wuhan University, Wuhan 430062, Hubei, China Abstract Background Alveolar macrophages (AMs) are crucial for defending against respiratory syncytial virus (RSV) and regulating lung inflammation.. Notch signaling is essential for macrophage polarization and its functional diversity.However, the role of macrophages Notch1 in RSV-induced lung injury and its underlying mechanisms remain incompletely understood.. Methods This study used macrophage-specific Notch1 knockout (MKO) mice to investigate the lung lesions and inflammation in RSV-infected mice.

    Article Title: Histone lactylation increases CXCL1 expression for neutrophil infiltration and immune escape in pancreatic cancer.
    Article Snippet: Then the cells were rinsed and treated with Fc receptor (BioLegend, #101320) blocking solution for 15 min. Then, the cells were incubated in fluorescence-labeled extracellular antibodies at 4°C in the dark for 30 minutes followed by twice rinsing to remove unbound antibodies. .. For Granzyme B staining, the cells were incubated with intracellular fixation/permeabilization buffer kit (Elabscience, #E-CK-A109). .. Finally, the cells were resuspended in 300 μl buffer (PBS + 1% FBS) and subjected to flow cytometry analysis performed on a DxFLEX flow cytometer (Beckman Coulter).

    Incubation:

    Article Title: Histone lactylation increases CXCL1 expression for neutrophil infiltration and immune escape in pancreatic cancer.
    Article Snippet: Then the cells were rinsed and treated with Fc receptor (BioLegend, #101320) blocking solution for 15 min. Then, the cells were incubated in fluorescence-labeled extracellular antibodies at 4°C in the dark for 30 minutes followed by twice rinsing to remove unbound antibodies. .. For Granzyme B staining, the cells were incubated with intracellular fixation/permeabilization buffer kit (Elabscience, #E-CK-A109). .. Finally, the cells were resuspended in 300 μl buffer (PBS + 1% FBS) and subjected to flow cytometry analysis performed on a DxFLEX flow cytometer (Beckman Coulter).



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