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Becton Dickinson permeabilization buffer fix/perm kit
Permeabilization Buffer Fix/Perm Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixation+permeabilization+buffer+kit/bioscience+fixation++permeabilization+buffer+kit/ppr0541637-235-70-76
Average 90 stars, based on 1 article reviews
permeabilization buffer fix/perm kit - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Galectin-3 Regulates Indoleamine-2,3-dioxygenase-Dependent Cross-Talk between Colon-Infiltrating Dendritic Cells and T Regulatory Cells and May Represent a Valuable Biomarker for Monitoring the Progression of Ulcerative Colitis
Article Snippet: Intracellular staining for forkhead box P3 (Foxp3) was performed using the BD Bioscience fixation/permeabilization buffer kit.

Article Title: Mesenchymal stem cells attenuate acute liver injury by altering ratio between interleukin 17 producing and regulatory natural killer T cells.
Article Snippet: Mesenchymal stem cells (MSCs) are, due to immunomodulatory characteristics, considered as novel agents in the treatment of immune-mediated acute liver failure.. Although it is known that MSCs can regulate activation of T lymphocytes, their capacity to modulate function of neutrophils and NKT cells, major interleukin (IL)-17 producing cells in acute liver injury, is still unknown.. By using two well established murine models of neutrophil and NKT cell mediated acute liver failure (induced by Carbon tetrachloride (CCl4) and alphagalactoceramide (α-GalCer)), we investigated molecular and cellular mechanisms involved in MSC-mediated modulation of IL-17 signaling during acute liver injury.

Article Title: Protective role of IL-33/ST2 axis in Con A-induced hepatitis.
Article Snippet: Department of Microbiology and Immunology, Centre for Molecular Medicine and Stem Cell Research, Faculty of Medicine, University of Kragujevac, Serbia; Department of Biochemistry, Centre for Molecular Medicine and Stem Cell Research, Faculty of Medicine, University of Kragujevac, Serbia; Department of Pathology, Centre for Molecular Medicine and Stem Cell Research, Faculty of Medicine, University of Kragujevac, Serbia

Article Title: Metabolic syndrome attenuates ulcerative colitis: Correlation with interleukin-10 and galectin-3 expression
Article Snippet: Intracellular staining for IL-10 and Foxp3 was performed using the BD Bioscience fixation/ permeabilization buffer kit.

Article Title: Vaccine co-display of CSP and Pfs230 on liposomes targeting two Plasmodium falciparum differentiation stages.
Article Snippet: The cells were washed with FASC buffer twice, then fixed with the fixation/ permeabilization buffer (BD cytofix/perm kit; Biosciences Cat. # 555028) for 10 min on ice.

Article Title: Indoleamine 2,3-dioxygenase-dependent expansion of T-regulatory cells maintains mucosal healing in ulcerative colitis
Article Snippet: Intracellular staining for Foxp3 was performed using the BD Bioscience fixation/ permeabilization buffer kit, following the manufacturer’s instructions.

Staining:

Article Title: Myeloid mechano-metabolic programming restricts anti-tumor immunity
Article Snippet: .. For intracellular staining, cells were restimulated in RPMI (Gibco, 11875085) supplemented with 10% FCS (Benchmark, NC1643060), Pen/Strep/Glut (Gibco, 10378016), and b-mercaptoethanol [50 μM] (Gibco, 21985023) containing phorbol 12-myristate 12-acetate (PMA) [50 ng/mL] (Sigma, P8139), ionomycin [500 ng/mL] (Invitrogen, I24222), brefeldin A (BFA) [3 mg/mL] (Sigma, B7651) in 5% CO2 at 37 oC for 3-5 h. After surface staining, cells were xed at 4 oC for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). .. Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 ºC for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer.



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