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FUJIFILM fixation buffer fm fix
Fixation Buffer Fm Fix, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixation+buffer+(fix/fixation+buffer+fm+fix/pm40306133-97-4-8
Average 90 stars, based on 1 article reviews
fixation buffer fm fix - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Staining:

Article Title: Bone marrow niches orchestrate stem cell hierarchy and immune tolerance
Article Snippet: To stain HSCs with MitoSOX (Invitrogen; 5 μM) or CYTO-ID (Enzo Life Sciences; × 500 dilution), each dye was added to the DAF-FM-containing culture media of freshly harvested BM cells and incubated for 20 min before staining with surface antibodies. .. For the intracellular staining, after cell surface staining and DAF-FM staining, BM cells were incubated overnight in fixation buffer FM Fix (Fujifilm) at 4°C. .. After washing, cells were incubated with permeabilization buffer (eBioscience; 2x) for 20 min at room temperature; washed once; and incubated again with permeabilization buffer (eBioscience; 2x) for 20 min. After washing, samples were stained for 60 min with the following antibodies: eNOS-Alexa Fluor 647 (Abcam) or rabbit IgG-Alexa Fluor 647 isotype (Abcam); nNOS-Alexa Fluor 647 (Abcam) or rabbit IgG-Alexa Fluor 647 isotype (Abcam); G-CSFR-PE (Novus Biologicals) or Rat IgG2a-PE isotype (Biolegend); CXCR4-PE (Biolegend) or rat IgG2b κ-PE isotype (Biolegend); Protective protein/Cathepsin A (CTSA)-Alexa Fluor 647 (Abcam) or rabbit IgG-Alexa Fluor 647 isotype (Abcam); LAMTOR1-Alexa Fluor 647 (Novus Biologicals) or rabbit IgG-Alexa Fluor 647 isotype (Novus Biologicals); or ROBLD3 (LAMTOR2)-Alexa Fluor 647 (Novus Biologicals) or rabbit IgG-Alexa Fluor 647 isotype (Novus Biologicals).

Incubation:

Article Title: Bone marrow niches orchestrate stem cell hierarchy and immune tolerance
Article Snippet: To stain HSCs with MitoSOX (Invitrogen; 5 μM) or CYTO-ID (Enzo Life Sciences; × 500 dilution), each dye was added to the DAF-FM-containing culture media of freshly harvested BM cells and incubated for 20 min before staining with surface antibodies. .. For the intracellular staining, after cell surface staining and DAF-FM staining, BM cells were incubated overnight in fixation buffer FM Fix (Fujifilm) at 4°C. .. After washing, cells were incubated with permeabilization buffer (eBioscience; 2x) for 20 min at room temperature; washed once; and incubated again with permeabilization buffer (eBioscience; 2x) for 20 min. After washing, samples were stained for 60 min with the following antibodies: eNOS-Alexa Fluor 647 (Abcam) or rabbit IgG-Alexa Fluor 647 isotype (Abcam); nNOS-Alexa Fluor 647 (Abcam) or rabbit IgG-Alexa Fluor 647 isotype (Abcam); G-CSFR-PE (Novus Biologicals) or Rat IgG2a-PE isotype (Biolegend); CXCR4-PE (Biolegend) or rat IgG2b κ-PE isotype (Biolegend); Protective protein/Cathepsin A (CTSA)-Alexa Fluor 647 (Abcam) or rabbit IgG-Alexa Fluor 647 isotype (Abcam); LAMTOR1-Alexa Fluor 647 (Novus Biologicals) or rabbit IgG-Alexa Fluor 647 isotype (Novus Biologicals); or ROBLD3 (LAMTOR2)-Alexa Fluor 647 (Novus Biologicals) or rabbit IgG-Alexa Fluor 647 isotype (Novus Biologicals).

Article Title: Transfer of bone marrow niche-residential regulatory T cells ameliorates experimental colitis.
Article Snippet: Background: Adoptive transfer of regulatory T cells (Tregs) has been proposed as a next-generation treatment approach for the treatment of various inflammatory or autoimmune disorders(Amini et al., 2022; Bluestone et al., 2023, 2015; Dall’Era et al., 2019; Chandran et al., 2017; Laukova and Glatman Zaretsky, 2023; Voskens et al., 2023; Canavan et al., 20161–8), inclusive of inflammatory bowel diseases (IBD).. Identification of the appropriate Treg populations as donor sources for effective cell therapy is of great importance.. We have recently identified specialized Tregs that localize within the hematopoietic stem cell (HSC) microenvironments(Fujisaki et al., 2011; Hirata et al., 2018, 2019, 2015; Kakiuchi et al., 2021a, 2021b; Furuhashi et al., 20259–16) of bone marrow (BM), termed HSC niches.



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