intercalation solution maxpar (fluidigm)

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fluidigm intercalation solution maxpar
Intercalation Solution Maxpar, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fix+perm+solution/Maxpar+Fix+and+Perm+Buffer/pm41663394-360-32-36
Average 95 stars, based on 300 article reviews

intercalation solution maxpar - by Bioz Stars, 2026-10

95/100 stars

Images

Labeling:

Article Title: Mutant KRAS vaccine with dual checkpoint blockade in resected pancreatic cancer: a phase I trial.
Article Snippet: Fully stained cells were washed with CSB and fixed with freshly prepared PierceTM paraformaldehyde 1.6% (ThermoFisher Scientific, cat#28906) in Maxpar PBS. .. On the day of acquisition, all cells were labeled with 103Rh (1:500, cat#201103, in Maxpar Fix & Perm Buffer, cat#201067, Standard BioTools) for 30min at RT. .. Data was acquired on HeliosTM at the Johns Hopkins Mass Cytometry Facility.

Staining:

Article Title: MIMIC: a flexible pipeline to register and summarize IMC-MSI experiments.
Article Snippet: .. After staining, cells were washed twice with phosphate-buffered saline (PBS) and then simultaneously fixed while staining DNA using Cell-ID Intercalator Ir (Standard BioTools PN: 201192B) diluted 1:1000 in Maxpar Fix and Perm Buffer (Standard BioTools PN: 201067) for thirty minutes. ..

Article Title: Tumour-intrinsic features shape T cell differentiation through precursor to symptomatic multiple myeloma
Article Snippet: .. Cells were then incubated with Cell-ID intercalator-Ir (Fluidigm Sciences) to stain all cells in MaxPar Fix and Perm Buffer (Fluidigm Sciences, 201067) and aliquoted and frozen in cryovials. ..

Article Title: Circulating pre-osteoclasts are primed for osteoclast fate and synovial tissue homing in psoriatic arthritis
Article Snippet: Cells were washed with 2ml Maxpar cell staining buffer and fixed in 1.6% formaldehyde, diluted from 16% methanol free formaldehyde (ThermoScientific, 28906) in Maxpar PBS (Standard Biotools, 201058), for 10 minutes at room temperature. .. Cells were pelleted, supernatant removed, and stained overnight at 4°C with 1ml of 125nM Cell-ID Intercalator Iridium (Standard Biotools, 201192A), in Maxpar Fix and Perm Solution (Standard Biotools, 201067). .. Cells were then transferred to 1.5ml LoBind Eppendorf’s, with care being taken to collect residual cells.

Saline:

FACS:

Article Title: Histone lactylation increases CXCL1 expression for neutrophil infiltration and immune escape in pancreatic cancer.
Article Snippet: Cells were washed in PBS, stained with 250 nM cisplatin (Fluidigm, # 201194) for 5 minutes on ice to exclude dead cells, and treated with Fc receptor blocking solution before incubation with a surface antibody cocktail on ice for 30 minutes. .. AR TI CL E IN P RE SS Following two washes with FACS buffer (PBS + 0.5% BSA), cells were fixed overnight in 200 μL intercalation solution (Maxpar Fix and Perm Buffer with 250 nM 191/193Ir, Fluidigm, #201067). ..

Article Title: IL-5 CAR-T cell therapy induces effective remission in hypereosinophilic disorders
Article Snippet: Cells were washed once with 1×PBS and then stained with 100 μL of 250 nM cisplatin (Fluidigm) for 5 min on ice to exclude dead cells, and then incubated in Fc receptor blocking solution before being stained with surface antibodies cocktail for 30 min on ice. .. Cells were washed twice with FACS buffer (1×PBS + 0.5% BSA) and fixed in 200 μL of intercalation solution (Maxpar Fix and Perm Buffer containing 250 nM 191 / 193 Ir, Fluidigm) overnight. .. After fixation, cells were washed once with FACS buffer and then perm buffer (eBioscience), stained with intracellular antibodies cocktail for 30 min on ice.

Article Title: Histone lactylation increases CXCL1 expression for neutrophil infiltration and immune escape in pancreatic cancer
Article Snippet: Cells were washed in PBS, stained with 250 nM cisplatin (Fluidigm, # 201194) for 5 min on ice to exclude dead cells, and treated with Fc receptor blocking solution before incubation with a surface antibody cocktail on ice for 30 min. .. Following two washes with FACS buffer (PBS + 0.5% BSA), cells were fixed overnight in 200 μL intercalation solution (Maxpar Fix and Perm Buffer with 250 nM 191/193Ir, Fluidigm, #201067). ..

Incubation:

other:

Article Title: Evaluation of nanobodies engineered with extra-cysteines for site-specific functionalization.
Article Snippet: Prior to the acquisition, cells were washed twice with CSB and twice with MaxPar Cell Acquisition Solution Plus (CAS PLUS, 201244, Standard Biotools) at 800 x g for 5 min at 4◦C, then resuspended at 6 × 105 per mL in CAS PLUS.

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