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First BASE Laboratories 5x ssc buffer
5x Ssc Buffer, supplied by First BASE Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/first+strand+buffer+5x/5x+ssc+buffer/pmc09758177-357-32-35
Average 90 stars, based on 1 article reviews
5x ssc buffer - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

other:

Article Title: Muscle 4EBP1 activation modifies the structure and function of the neuromuscular junction in mice
Article Snippet: Image analysis was performed using Imaris software as per method used for quantification of NMJ myonuclei.

Incubation:

Article Title: Muscle 4EBP1 activation modifies the structure and function of the neuromuscular junction in mice.
Article Snippet: Image analysis was performed using Imaris software as per method used for quantification of NMJ myonuclei. .. After which, the sectionswere treatedwith kit-provided Protease IV, followed by incubation with the respective RNAScope probes (Supplementary Table S3) at 40 °C for 2 h. The sections were stored in 5X SSC buffer (1st Base, BUF-3051) overnight, and the amplification andmultiplexing steps took place the next day. .. Acetylcholine receptor turnover was assessed by intramuscular injection of BTX-AF647 (25 pmol in 0.5μl; Invitrogen, B35450) and BTX-AF488 (25pmol in 0.5μl) into the tibialis anterior muscle on days 1 and 10, respectively.

RNAscope:

Article Title: Muscle 4EBP1 activation modifies the structure and function of the neuromuscular junction in mice.
Article Snippet: Image analysis was performed using Imaris software as per method used for quantification of NMJ myonuclei. .. After which, the sectionswere treatedwith kit-provided Protease IV, followed by incubation with the respective RNAScope probes (Supplementary Table S3) at 40 °C for 2 h. The sections were stored in 5X SSC buffer (1st Base, BUF-3051) overnight, and the amplification andmultiplexing steps took place the next day. .. Acetylcholine receptor turnover was assessed by intramuscular injection of BTX-AF647 (25 pmol in 0.5μl; Invitrogen, B35450) and BTX-AF488 (25pmol in 0.5μl) into the tibialis anterior muscle on days 1 and 10, respectively.

Amplification:

Article Title: Muscle 4EBP1 activation modifies the structure and function of the neuromuscular junction in mice.
Article Snippet: Image analysis was performed using Imaris software as per method used for quantification of NMJ myonuclei. .. After which, the sectionswere treatedwith kit-provided Protease IV, followed by incubation with the respective RNAScope probes (Supplementary Table S3) at 40 °C for 2 h. The sections were stored in 5X SSC buffer (1st Base, BUF-3051) overnight, and the amplification andmultiplexing steps took place the next day. .. Acetylcholine receptor turnover was assessed by intramuscular injection of BTX-AF647 (25 pmol in 0.5μl; Invitrogen, B35450) and BTX-AF488 (25pmol in 0.5μl) into the tibialis anterior muscle on days 1 and 10, respectively.



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