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Promega wizard’s resin filter method
Wizard’s Resin Filter Method, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/filter+wizard/wizard+resin/pm12883879-35-7-10
Average 90 stars, based on 1 article reviews
wizard’s resin filter method - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: Identification of RNA–DNA Hybrids Associated with R-Loops at the IgH Switch Sequence in Activated B Cells
Article Snippet: Incubate the microfuge tube in the dark at 37 °C for 12–16 h. Purify bisulfite converted DNA using 1 mL of Wizard resin (Promega) or a DNA spin column (Zymo Research) according to the manufacturer’s instructions.

Article Title: Highly diverse flavobacterial phages as mortality factor during North Sea spring blooms
Article Snippet: Phage DNA was extracted using the Wizard resin kit (Promega, Madison, USA) and eluted in TE buffer (after ( )).

Ethanol Precipitation:

Article Title: Identification of RNA–DNA Hybrids Associated with R-Loops at the IgH Switch Sequence in Activated B Cells
Article Snippet: .. Purify desulfonated DNA by ethanol precipitation, or, Wizard resin (Promega) or DNA spin column (Zymo Research). ..

Purification:

Article Title: Schizophrenia-Linked Protein tSNARE1 Regulates Endosomal Trafficking in Cortical Neurons
Article Snippet: The primers (Eurofins) used for TSNARE1 transcript amplification are as follows: TSN1-NT3 (59-gcatcaggatccaaatcgcccgtggaggtggc-39), TSN1-CT3 (59- gcatcagtctagatcaggggcagccacaagcagctgggggt-39), and TSN1-CT2b (59-gca tcagtctagatcactttcggacagaggtggcgatgatgatga-39); 1ml cDNA was amplified using 1mL Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer with 10 mM primers, 200 mM dNTPs (Agilent), 65% DMSO per 50ml reaction. .. Transcripts were checked on agarose gels, purified with phenol:chloroform:isoamyl alcohol and Wizard resin (Promega), digested with XbaI and BamHI-HF in Cutsmart Buffer (NEB), and gel purified using Gel Extraction Kit (QIAGEN). .. Isolated transcripts were ligated into pEGFP-C2 using Quick Ligation Kit (NEB), cloned into DH5a, purified with Wizard Genomic DNA Purification kit (Promega), and confirmed via Sanger sequencing (GeneWiz).

Gel Extraction:

Article Title: Schizophrenia-Linked Protein tSNARE1 Regulates Endosomal Trafficking in Cortical Neurons
Article Snippet: The primers (Eurofins) used for TSNARE1 transcript amplification are as follows: TSN1-NT3 (59-gcatcaggatccaaatcgcccgtggaggtggc-39), TSN1-CT3 (59- gcatcagtctagatcaggggcagccacaagcagctgggggt-39), and TSN1-CT2b (59-gca tcagtctagatcactttcggacagaggtggcgatgatgatga-39); 1ml cDNA was amplified using 1mL Phusion High-Fidelity DNA Polymerase (NEB) in HF Phusion buffer with 10 mM primers, 200 mM dNTPs (Agilent), 65% DMSO per 50ml reaction. .. Transcripts were checked on agarose gels, purified with phenol:chloroform:isoamyl alcohol and Wizard resin (Promega), digested with XbaI and BamHI-HF in Cutsmart Buffer (NEB), and gel purified using Gel Extraction Kit (QIAGEN). .. Isolated transcripts were ligated into pEGFP-C2 using Quick Ligation Kit (NEB), cloned into DH5a, purified with Wizard Genomic DNA Purification kit (Promega), and confirmed via Sanger sequencing (GeneWiz).



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