Review



mouse fgf21  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    R&D Systems mouse fgf21
    GDF15 Expression Is Regulated by the Cellular ISR Pathway (A and C) GDF15 mRNA expression (A) and immunoblot analysis (C) of ISR components in wild-type (WT) mouse embryonic fibroblasts (MEFs) treated with vehicle control (Con), cobalt chloride (CoCl2, 625 μM), thapsigargin (Tg, 1 μM), tunicamycin (Tn 5 μg/mL), or L-Histidinol (His, 1 mM) for 6 h. (B) GDF15 mRNA expression in human cell lines (HeLA, HuH7, and A549) treated with Tn (5 μg/mL) for 6 h. (D) GDF15 mRNA expression in WT MEFs pre-treated for 1 h either with the PERK inhibitor GSK2606414 (GSK, 200 nM) or eIF2α inhibitor ISRIB (ISR, 100 nM), then co-treated with Tn (5 μg/mL) for a further 6 h. (E–G) GDF15 mRNA expression (E) in EIF2α Ser51 (SS) or phospho mutant (AA) MEFs or (F) in ATF4 wild-type (WT) or ATF4 knockout (KO) MEFs and (G) in control siRNA and CHOP siRNA transfected WT MEFs treated with Tn (5 μg/mL) for 6 h. (H) Diagram outlining pathway by which GDF15 and <t>FGF21</t> expression is regulated by TN. mRNA expression is presented as fold expression relative to its respective control treatment for each cell type (set at 1) or TN-treated samples (set as 100) with normalization to HPRT gene expression in MEFs and GAPDH in human cells. Data are expressed as mean ± SD from at least three independent experiments. ∗∗∗ p < 0.001 versus control (con) for (A) and (B), and versus TN stimulated for (D)–(G) by two-tailed Student’s t test. Blots shown are representative of three independent experiments with Calnexin used as a loading control. See also <xref ref-type=Figures S3 and . " width="250" height="auto" />
    Mouse Fgf21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Recombinant+Mouse+FGF-21+Protein%2C+CF/pmc06408327-537-0-8
    Average 92 stars, based on 7 article reviews
    mouse fgf21 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans"

    Article Title: GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans

    Journal: Cell Metabolism

    doi: 10.1016/j.cmet.2018.12.016

    GDF15 Expression Is Regulated by the Cellular ISR Pathway (A and C) GDF15 mRNA expression (A) and immunoblot analysis (C) of ISR components in wild-type (WT) mouse embryonic fibroblasts (MEFs) treated with vehicle control (Con), cobalt chloride (CoCl2, 625 μM), thapsigargin (Tg, 1 μM), tunicamycin (Tn 5 μg/mL), or L-Histidinol (His, 1 mM) for 6 h. (B) GDF15 mRNA expression in human cell lines (HeLA, HuH7, and A549) treated with Tn (5 μg/mL) for 6 h. (D) GDF15 mRNA expression in WT MEFs pre-treated for 1 h either with the PERK inhibitor GSK2606414 (GSK, 200 nM) or eIF2α inhibitor ISRIB (ISR, 100 nM), then co-treated with Tn (5 μg/mL) for a further 6 h. (E–G) GDF15 mRNA expression (E) in EIF2α Ser51 (SS) or phospho mutant (AA) MEFs or (F) in ATF4 wild-type (WT) or ATF4 knockout (KO) MEFs and (G) in control siRNA and CHOP siRNA transfected WT MEFs treated with Tn (5 μg/mL) for 6 h. (H) Diagram outlining pathway by which GDF15 and FGF21 expression is regulated by TN. mRNA expression is presented as fold expression relative to its respective control treatment for each cell type (set at 1) or TN-treated samples (set as 100) with normalization to HPRT gene expression in MEFs and GAPDH in human cells. Data are expressed as mean ± SD from at least three independent experiments. ∗∗∗ p < 0.001 versus control (con) for (A) and (B), and versus TN stimulated for (D)–(G) by two-tailed Student’s t test. Blots shown are representative of three independent experiments with Calnexin used as a loading control. See also <xref ref-type=Figures S3 and . " title="... (H) Diagram outlining pathway by which GDF15 and FGF21 expression is regulated by TN. mRNA expression is ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: GDF15 Expression Is Regulated by the Cellular ISR Pathway (A and C) GDF15 mRNA expression (A) and immunoblot analysis (C) of ISR components in wild-type (WT) mouse embryonic fibroblasts (MEFs) treated with vehicle control (Con), cobalt chloride (CoCl2, 625 μM), thapsigargin (Tg, 1 μM), tunicamycin (Tn 5 μg/mL), or L-Histidinol (His, 1 mM) for 6 h. (B) GDF15 mRNA expression in human cell lines (HeLA, HuH7, and A549) treated with Tn (5 μg/mL) for 6 h. (D) GDF15 mRNA expression in WT MEFs pre-treated for 1 h either with the PERK inhibitor GSK2606414 (GSK, 200 nM) or eIF2α inhibitor ISRIB (ISR, 100 nM), then co-treated with Tn (5 μg/mL) for a further 6 h. (E–G) GDF15 mRNA expression (E) in EIF2α Ser51 (SS) or phospho mutant (AA) MEFs or (F) in ATF4 wild-type (WT) or ATF4 knockout (KO) MEFs and (G) in control siRNA and CHOP siRNA transfected WT MEFs treated with Tn (5 μg/mL) for 6 h. (H) Diagram outlining pathway by which GDF15 and FGF21 expression is regulated by TN. mRNA expression is presented as fold expression relative to its respective control treatment for each cell type (set at 1) or TN-treated samples (set as 100) with normalization to HPRT gene expression in MEFs and GAPDH in human cells. Data are expressed as mean ± SD from at least three independent experiments. ∗∗∗ p < 0.001 versus control (con) for (A) and (B), and versus TN stimulated for (D)–(G) by two-tailed Student’s t test. Blots shown are representative of three independent experiments with Calnexin used as a loading control. See also Figures S3 and .

    Techniques Used: Expressing, Western Blot, Control, Mutagenesis, Knock-Out, Transfection, Gene Expression, Two Tailed Test


    Figure Legend Snippet:

    Techniques Used: Recombinant, SYBR Green Assay, Protease Inhibitor, Reverse Transcription, Western Blot, Injection, Enzyme-linked Immunosorbent Assay, Control, TaqMan Assay, Software, Sterility, Electrophoresis, Real-time Polymerase Chain Reaction

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Combined genetic deletion of GDF15 and FGF21 has modest effects on body weight, hepatic steatosis and insulin resistance in high fat fed mice
    Article Snippet: Mouse GDF15 was measured using a DuoSet ELISA (R&D Systems product code DY6385) which had been modified to run as an electrochemiluminescence assay on the MesoScale Discovery assay platform. .. Mouse FGF21 was analysed using a Quantikine ELISA kit (R&D Systems, product code MF2100) following the manufacturer’s instructions. .. ALT, AST, Cholesterol and triglycerides were measured on the Siemens Dimension EXL autoanalyser using Siemens reagents and calibrators.

    Article Title: GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans
    Article Snippet: Mouse GDF15 was measured using a Mouse GDF15 DuoSet ELISA (R&D Systems) which had been modified to run as an electrochemiluminescence assay on the Meso Scale Discovery assay platform. .. Mouse FGF21 was analyzed by FGF21 Quantakine ELISA (R&D Systems) following the manufacturer’s instructions. ..



    Similar Products

    fgf21  (MedChemExpress)
    93
    MedChemExpress fgf21
    Fgf21, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/FGF-21%2C+Human/pm42302645-56-3-17
    Average 93 stars, based on 1 article reviews
    fgf21 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    fgf21  (Multi Sciences (Lianke) Biotech Co Ltd)
    90
    Multi Sciences (Lianke) Biotech Co Ltd fgf21
    Fgf21, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Mouse+FGF-21+ELISA+Kit/pm41967182-155-19-22
    Average 90 stars, based on 1 article reviews
    fgf21 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    gene exp fgf21 hs00173927 m1  (Thermo Fisher)
    92
    Thermo Fisher gene exp fgf21 hs00173927 m1
    Gene Exp Fgf21 Hs00173927 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Gene+Exp%2E+FGF21%2C+Hs00173927_m1/us12590307-1357-43-44
    Average 92 stars, based on 1 article reviews
    gene exp fgf21 hs00173927 m1 - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier
    fgf21 analogue pegozafermin  (Sanyal Biotechnology)
    86
    Sanyal Biotechnology fgf21 analogue pegozafermin
    Fgf21 Analogue Pegozafermin, supplied by Sanyal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/analogue+fgf21+pegozafermin/10__1161_slash_circresaha__126__327270-597-27-2
    Average 86 stars, based on 1 article reviews
    fgf21 analogue pegozafermin - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    fgf21 ko mice to streptozotocin stz  (MedChemExpress)
    97
    MedChemExpress fgf21 ko mice to streptozotocin stz
    Fgf21 Ko Mice To Streptozotocin Stz, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Streptozotocin/10__31083_slash_fbl47702-53-10-17
    Average 97 stars, based on 1 article reviews
    fgf21 ko mice to streptozotocin stz - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    exogenous fgf21  (MedChemExpress)
    94
    MedChemExpress exogenous fgf21
    Exogenous Fgf21, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/FGF-21%2C+Mouse/10__1016_slash_j__matdes__2026__115991-138-5-13
    Average 94 stars, based on 1 article reviews
    exogenous fgf21 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    fibroblast growth factor 21 fgf21 knockout knockout ko mice  (Jackson Laboratory)
    86
    Jackson Laboratory fibroblast growth factor 21 fgf21 knockout knockout ko mice
    Fibroblast Growth Factor 21 Fgf21 Knockout Knockout Ko Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/adiponectin+cre+mice/10__1113_slash_jp289817-70-0-9
    Average 86 stars, based on 1 article reviews
    fibroblast growth factor 21 fgf21 knockout knockout ko mice - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    mouse rat fgf21 quantikine elisa kit  (R&D Systems)
    96
    R&D Systems mouse rat fgf21 quantikine elisa kit
    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
    Mouse Rat Fgf21 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Mouse%2FRat+FGF-21+Quantikine+ELISA+Kit/bio_rxiv__64898__2026__03__31__715667-55-22-29
    Average 96 stars, based on 1 article reviews
    mouse rat fgf21 quantikine elisa kit - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    c plasma fgf21 protein levels  (Proteintech)
    93
    Proteintech c plasma fgf21 protein levels
    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
    C Plasma Fgf21 Protein Levels, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Mouse+FGF-21+ELISA+Kit/us12590307-1321-43-60
    Average 93 stars, based on 1 article reviews
    c plasma fgf21 protein levels - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Journal: bioRxiv

    Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice

    doi: 10.64898/2026.03.31.715667

    Figure Lengend Snippet: (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Article Snippet: Blood for circulating FGF21, testosterone, and estradiol levels was collected from submandibular bleeding immediately prior to euthanasia; plasma was assayed with a mouse/rat FGF21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA), and testosterone (582701) and estradiol (501890) quantikine ELISA kits from Caymen Chemicals (Ann Arbor, MI, USA).

    Techniques: Activity Assay, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control